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Glycolytic enzymes are crucial molecules for the survival and development of <jats:italic>E. granulosus<\/jats:italic>. The aim of this study was to investigate the molecular characterization, immunogenicity, tissue distribution and serodiagnostic potential of <jats:italic>E. granulosus<\/jats:italic> hexokinase (<jats:italic>Eg<\/jats:italic>HK), the first key enzyme in the glycolytic pathway.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p><jats:italic>Eg<\/jats:italic>HK was cloned and expressed in <jats:italic>Escherichia coli<\/jats:italic>. Specific serum antibodies were evaluated in mice immunized with recombinant <jats:italic>Eg<\/jats:italic>HK (r<jats:italic>Eg<\/jats:italic>HK). The location of <jats:italic>Eg<\/jats:italic>HK in the larval stage of <jats:italic>E. granulosus<\/jats:italic> was determined using fluorescence immunohistochemistry, and the potential of r<jats:italic>Eg<\/jats:italic>HK as a diagnostic antigen was investigated in patients with CE using indirect enzyme-linked immunosorbent assay (ELISA).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Recombinant <jats:italic>Eg<\/jats:italic>HK could be identified in the sera of patients with CE and in mouse anti-r<jats:italic>Eg<\/jats:italic>HK sera. High titers of specific immunoglobulin G were induced in mice after immunization with r<jats:italic>Eg<\/jats:italic>HK. <jats:italic>Eg<\/jats:italic>HK was mainly located in the tegument, suckers and hooklets of protoscoleces and in the germinal layer and laminated layer of the cyst wall. The sensitivity and specificity of the r<jats:italic>Eg<\/jats:italic>HK-ELISA reached 91.3% (42\/46) and 87.8% (43\/49), respectively.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>We have characterized the sequence, structure and location of <jats:italic>Eg<\/jats:italic>HK and investigated the immunoreactivity, immunogenicity and serodiagnostic potential of r<jats:italic>Eg<\/jats:italic>HK. Our results suggest that<jats:italic> Eg<\/jats:italic>HK may be a promising candidate for the development of vaccines against <jats:italic>E. granulosus<\/jats:italic> and an effective antigen for the diagnosis of human CE.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-021-04606-8","type":"journal-article","created":{"date-parts":[[2021,2,8]],"date-time":"2021-02-08T22:50:31Z","timestamp":1612824631000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Molecular characterization and serodiagnostic potential of Echinococcus granulosus hexokinase"],"prefix":"10.1186","volume":"14","author":[{"given":"Qi","family":"Xin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaomiao","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanping","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxia","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Jing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,2,8]]},"reference":[{"key":"4606_CR1","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1186\/s40249-016-0109-x","volume":"5","author":"AM Cadavid Restrepo","year":"2016","unstructured":"Cadavid Restrepo AM, Yang YR, McManus DP, Gray DJ, Giraudoux P, Barnes TS, et al. 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