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Free-ranging carnivores can act as sentinel species for detection of <jats:italic>Trichinella<\/jats:italic> spp. Knowledge of the epidemiology of these parasites may help prevent <jats:italic>Trichinella<\/jats:italic> spp. infections in northern Canadian animals and people. Previous research on <jats:italic>Trichinella<\/jats:italic> spp. in wildlife from Yukon did not identify risk factors associated with infection, or the diversity and identity of species of <jats:italic>Trichinella<\/jats:italic> in regional circulation, based on geographically extensive sampling with large sample sizes.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>In a cross-sectional study, we determined the prevalence, infection intensity, risk factors, and species or genotypes of <jats:italic>Trichinella<\/jats:italic> in wolverine (<jats:italic>Gulo gulo<\/jats:italic>) in two regions of Yukon, Canada, from 2013\u20132017. A double separatory funnel digestion method followed by mutiplex PCR and PCR-RFLP were used to recover and identify species of <jats:italic>Trichinella<\/jats:italic>, respectively.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>We found larvae of <jats:italic>Trichinella<\/jats:italic> in the tongues of 78% (95% CI 73\u201382) of 338 wolverine sampled. The odds of adult (\u2265\u20092\u00a0years) and yearling (1 year) wolverine being <jats:italic>Trichinella<\/jats:italic> spp.-positive were four and two times higher, respectively, compared to juveniles (&lt;1 year).\u00a0The odds of <jats:italic>Trichinella<\/jats:italic> spp<jats:italic>.<\/jats:italic> presence were three times higher in wolverine from southeast than northwest Yukon. The mean intensity of infection was 22.6\u2009\u00b1\u200939 (SD, range 0.1\u2013295) larvae per gram. <jats:italic>Trichinella<\/jats:italic> T6 was the predominant genotype (76%), followed by <jats:italic>T. nativa<\/jats:italic> (8%); mixed infections with <jats:italic>Trichinella<\/jats:italic> T6 and <jats:italic>T. nativa<\/jats:italic> (12%) were observed. In addition, <jats:italic>T. spiralis<\/jats:italic> was detected in one wolverine. Out of 22 isolates initially identified as <jats:italic>T. nativa<\/jats:italic> in multiplex PCR, 14 were analyzed by PCR-RFLP to distinguish them from <jats:italic>T. chanchalensis<\/jats:italic>, a recently discovered cryptic species, which cannot be distinguished from the <jats:italic>T. nativa<\/jats:italic> on multiplex PCR. Ten isolates were identified either as <jats:italic>T. chanchalensis<\/jats:italic> alone (<jats:italic>n<\/jats:italic>\u2009=\u20097), or mixed infection with <jats:italic>T. chanchalensis<\/jats:italic> and <jats:italic>T. nativa<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u20092) or <jats:italic>T. chanchalensis<\/jats:italic> and <jats:italic>Trichinella<\/jats:italic> T6 (<jats:italic>n<\/jats:italic>\u2009=\u20091)].<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Wolverine hosted high prevalence, high larval intensity, and multiple species of <jats:italic>Trichinella<\/jats:italic>, likely due to their scavenging habits, apex position in the food chain, and wide home range. Wolverine (especially adult males) should be considered as a sentinel species for surveys for <jats:italic>Trichinella<\/jats:italic> spp. across their distributional range.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-021-04636-2","type":"journal-article","created":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T11:03:50Z","timestamp":1615201430000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["High prevalence, intensity, and genetic diversity of Trichinella spp. in wolverine (Gulo gulo) from Yukon, Canada"],"prefix":"10.1186","volume":"14","author":[{"given":"Rajnish","family":"Sharma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. 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