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They have been an issue of growing importance in recent years; however, there is limited information about the vector-borne pathogens circulating in Portugal. The aim of the present study was to detect canine vector-borne bacteria and protozoa of veterinary and zoonotic importance using molecular methods.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>One hundred and forty-two dogs from Lisbon, southern Portugal, were tested: 48 dogs from a veterinary hospital clinically suspected of vector-borne diseases and 94 apparently healthy dogs from shelters. <jats:italic>Anaplasma<\/jats:italic> spp.\/<jats:italic>Ehrlichia<\/jats:italic> spp., <jats:italic>Babesia\/Theileria<\/jats:italic> spp., <jats:italic>Hepatozoon<\/jats:italic> spp., and <jats:italic>Mycoplasma<\/jats:italic> spp. infections were detected by PCR from blood samples and examined under light microscopy. Other information including clinical status and diagnostic test results were collected for each animal.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Infections were detected by PCR in 48 (33.80%) dogs. Single infections were found in 35 dogs (24.64%), and co-infections were found in 13 (9.15%) dogs. Twenty-nine (20.42%) dogs were positive for <jats:italic>Hepatozoon<\/jats:italic> spp., 15 (10.56%) for <jats:italic>Mycoplasma<\/jats:italic> spp., 11 (7.75%) for <jats:italic>Anaplasma<\/jats:italic> spp.\/<jats:italic>Ehrlichia<\/jats:italic> spp., and six (4.21%) for <jats:italic>Babesia<\/jats:italic> spp. DNA sequencing was used to identify <jats:italic>Babesia vogeli<\/jats:italic> (2.81%), <jats:italic>Babesia canis<\/jats:italic> (1.40%), <jats:italic>Hepatozoon canis<\/jats:italic> (20.42%), <jats:italic>Mycoplasma haematoparvum<\/jats:italic> (2.11%), <jats:italic>Mycoplasma haemocanis<\/jats:italic> (8.45%), <jats:italic>Anaplasma platys<\/jats:italic> (7.04%), and <jats:italic>Ehrlichia canis<\/jats:italic> (0.70%).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>This is the first molecular identification of <jats:italic>B. canis<\/jats:italic> and <jats:italic>M. haematoparvum<\/jats:italic> in dogs from southern Portugal. This study highlights the importance of molecular methods to identify CVBD pathogens in endemic areas and helps to guide the clinical approach of veterinarians in practice.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-021-04650-4","type":"journal-article","created":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T19:13:08Z","timestamp":1616094788000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal"],"prefix":"10.1186","volume":"14","author":[{"given":"Ana Mafalda","family":"Dordio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Relja","family":"Beck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Telmo","family":"Nunes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Pereira da Fonseca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacinto","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,18]]},"reference":[{"key":"4650_CR1","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1186\/1756-3305-4-55","volume":"4","author":"D Otranto","year":"2011","unstructured":"Otranto D, Dantas-Torres F, Weigl S, Latrofa MS, Stanneck D, Decaprariis D, Capelli G, Baneth G. 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