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Here we aimed to molecularly detect rickettsial agents in red fox (\n                      <jats:italic>Vulpes vulpes<\/jats:italic>\n                      ), marbled polecat (\n                      <jats:italic>Vormela peregusna<\/jats:italic>\n                      ) and their ticks.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      During 2018\u20132019, 12 red foxes, one marbled polecat and their ticks were sampled in two counties and a city of the XUAR. The heart, liver, spleen, lung and kidney of these 13 carnivores were dissected, followed by DNA extraction. Hard ticks were identified both morphologically and molecularly. All samples were examined for the presence of rickettsiae by amplifying four genetic markers (\n                      <jats:italic>17-kDa, gltA, ompA, sca1<\/jats:italic>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 26 adult ticks and 28 nymphs (38\n                      <jats:italic>Ixodes canisuga<\/jats:italic>\n                      , nine\n                      <jats:italic>Ixodes kaiseri<\/jats:italic>\n                      , six\n                      <jats:italic>Haemaphysalis erinacei<\/jats:italic>\n                      and one\n                      <jats:italic>Dermacentor marginatus<\/jats:italic>\n                      ) were collected from red foxes, and four\n                      <jats:italic>Ha. erinacei<\/jats:italic>\n                      ticks were removed from the marbled polecat. Analysis of cytochrome\n                      <jats:italic>c<\/jats:italic>\n                      oxidase subunit I (\n                      <jats:italic>COI<\/jats:italic>\n                      ) gene sequences indicated that 2\u201332 nucleotides differed between\n                      <jats:italic>I. canisuga<\/jats:italic>\n                      ,\n                      <jats:italic>I. kaiseri<\/jats:italic>\n                      and\n                      <jats:italic>Ha. erinacei<\/jats:italic>\n                      from northwestern China and Europe.\n                      <jats:italic>Rickettsia raoultii<\/jats:italic>\n                      was detected in three red foxes,\n                      <jats:italic>Candidatus<\/jats:italic>\n                      Rickettsia barbariae in a red fox,\n                      <jats:italic>Rickettsia sibirica<\/jats:italic>\n                      in a red fox and a marbled polecat, and\n                      <jats:italic>R. raoultii<\/jats:italic>\n                      in two tick species (\n                      <jats:italic>I. canisuga<\/jats:italic>\n                      and\n                      <jats:italic>D<\/jats:italic>\n                      .\n                      <jats:italic>marginatus<\/jats:italic>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      To the best of our knowledge,\n                      <jats:italic>I. canisuga<\/jats:italic>\n                      and\n                      <jats:italic>I. kaiseri<\/jats:italic>\n                      have not been previously reported from red foxes in China. The DNA of\n                      <jats:italic>R. sibirica<\/jats:italic>\n                      and\n                      <jats:italic>R. raoultii<\/jats:italic>\n                      was detected for the first time in the organs of red foxes, and\n                      <jats:italic>R. sibirica<\/jats:italic>\n                      in the organs of a marbled polecat. This is also the first molecular evidence for the presence of\n                      <jats:italic>R. raoultii<\/jats:italic>\n                      in\n                      <jats:italic>I. canisuga<\/jats:italic>\n                      . Our findings expand the range of tick-borne pathogens in wildlife species and associated ticks in China.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Graphical Abstract<\/jats:title>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-021-04718-1","type":"journal-article","created":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T05:03:16Z","timestamp":1618808596000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Rickettsiae in red fox (Vulpes vulpes), marbled polecat (Vormela peregusna) and their ticks in northwestern China"],"prefix":"10.1186","volume":"14","author":[{"given":"Gang","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e1ndor","family":"Hornok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wumei","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ligu","family":"Mi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suwen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wurelihazi","family":"Hazihan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinli","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanzhi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,4,19]]},"reference":[{"key":"4718_CR1","doi-asserted-by":"crossref","DOI":"10.56021\/9780801882210","volume-title":"Mammal species of the world. 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