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LO1503"],"award-info":[{"award-number":["Nr. LO1503"]}]},{"name":"NutRisk Centre","award":["CZ.02.1.01\/0.0\/0.0\/16_019\/0000845"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_019\/0000845"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>The blood flukes of genus <jats:italic>Schistosoma<\/jats:italic> are the causative agent of schistosomiasis, a parasitic disease that infects more than 200 million people worldwide. Proteases of schistosomes are involved in critical steps of host\u2013parasite interactions and are promising therapeutic targets. We recently identified and characterized a group of S1 family <jats:italic>Schistosoma mansoni<\/jats:italic> serine proteases, including SmSP1 to SmSP5. Expression levels of some SmSPs in <jats:italic>S. mansoni<\/jats:italic> are low, and by standard genome sequencing technologies they are marginally detectable at the method threshold levels. Here, we report their spatial gene expression patterns in adult <jats:italic>S. mansoni<\/jats:italic> by the high-sensitivity localization assay.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methodology<\/jats:title>\n                <jats:p>Highly sensitive fluorescence in situ RNA hybridization (FISH) was modified and used for the localization of mRNAs encoding individual SmSP proteases (including low-expressed SmSPs) in tissues of adult worms. High sensitivity was obtained due to specifically prepared tissue and probes in combination with the employment of a signal amplification approach. The assay method was validated by detecting the expression patterns of a set of relevant reference genes including SmCB1, SmPOP, SmTSP-2, and Sm29 with localization formerly determined by other techniques.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>FISH analysis revealed interesting expression patterns of SmSPs distributed in multiple tissues of <jats:italic>S. mansoni<\/jats:italic> adults. The expression patterns of individual SmSPs were distinct but in part overlapping and were consistent with existing transcriptome sequencing data. The exception were genes with significantly low expression, which were also localized in tissues where they had not previously been detected by RNA sequencing methods. In general, SmSPs were found in various tissues including reproductive organs, parenchymal cells, esophagus, and the tegumental surface.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The FISH-based assay provided spatial information about the expression of five SmSPs in adult <jats:italic>S. mansoni<\/jats:italic> females and males. This highly sensitive method allowed visualization of low-abundantly expressed genes that are below the detection limits of standard in situ hybridization or by RNA sequencing. Thus, this technical approach turned out to be suitable for sensitive localization studies and may also be applicable for other trematodes. The results suggest that SmSPs may play roles in diverse processes of the parasite. Certain SmSPs expressed at the surface may be involved in host\u2013parasite interactions.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-021-04773-8","type":"journal-article","created":{"date-parts":[[2021,5,22]],"date-time":"2021-05-22T11:02:35Z","timestamp":1621681355000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Spatial expression pattern of serine proteases in the blood fluke Schistosoma mansoni determined by fluorescence RNA in situ hybridization"],"prefix":"10.1186","volume":"14","author":[{"given":"Lenka","family":"Ulrychov\u00e1","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavel","family":"Osta\u0161ov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Chanov\u00e1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Mare\u0161","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9110-2018","authenticated-orcid":false,"given":"Martin","family":"Horn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Dvo\u0159\u00e1k","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,5,22]]},"reference":[{"key":"4773_CR1","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1038\/s41572-018-0013-8","volume":"4","author":"DP McManus","year":"2018","unstructured":"McManus DP, Dunne DW, Sacko M, Utzinger J, Vennervald BJ, Zhou XN. 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This project, including the use of experimental animals for the present study, was approved by the Ministry of Education, Youth and Sports of the Czech Republic (Approval Number MSMT-7063\/2017-2). All animals used in the study were maintained by a certified person (Certificate Number CZ 02627) in specifically accredited laboratories of the Institute of Immunology and Microbiology of the First Faculty of Medicine, Charles University and the General University Hospital in Prague (Accreditation Numbers 70030\/2013-MZE-17214 and 8615\/2019-MZE-17214), both issued by the Ministry of Agriculture of the Czech Republic.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"274"}}