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Little is known about the prevalence of\n                      <jats:italic>B. miyamotoi<\/jats:italic>\n                      infections in humans and ticks in Inner Mongolia, China. Therefore, we investigated the prevalence of\n                      <jats:italic>B. miyamotoi<\/jats:italic>\n                      in\n                      <jats:italic>Ixodes persulcatus<\/jats:italic>\n                      ticks, and we aimed to isolate\n                      <jats:italic>B. miyamotoi<\/jats:italic>\n                      from\n                      <jats:italic>I. persulcatus<\/jats:italic>\n                      from four regions of Greater Khingan, Inner Mongolia, China.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      From May to June each year during the period 2016\u20132019, host-seeking adult\n                      <jats:italic>I. persulcatus<\/jats:italic>\n                      ticks were collected from vegetation. Genomic DNA was prepared from half of each tick body for PCR template, and the remaining half was used to cultivate\n                      <jats:italic>B. miyamotoi<\/jats:italic>\n                      in BSK-M medium. We employed quantitative real-time PCR (qPCR) to detect\n                      <jats:italic>Borrelia<\/jats:italic>\n                      DNA in the ticks and to calculate the prevalence of\n                      <jats:italic>B. miyamotoi<\/jats:italic>\n                      and infections with other borreliae. For characterization of the isolated\n                      <jats:italic>B. miyamotoi<\/jats:italic>\n                      , we performed draft genome sequencing and multilocus sequencing analysis (MLSA).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 2656 adult\n                      <jats:italic>I. persulcatus<\/jats:italic>\n                      ticks were collected. The overall prevalence of relapsing fever (RF) borreliae in ticks was 5.0% (134\/2656) and that of Lyme disease (LD) borreliae was 43.8% (1164\/2656). Co-infection with RF and LD borreliae was observed in 63 ticks (2.4%). Ticks that were positive for RF borreliae by qPCR were subjected to glycerophosphodiester diester phosphodiesterase gene (\n                      <jats:italic>glpQ<\/jats:italic>\n                      ) PCR amplification and sequencing, through which we identified the RF borrelia specimens as\n                      <jats:italic>B. miyamotoi<\/jats:italic>\n                      . Furthermore, the\n                      <jats:italic>B. miyamotoi<\/jats:italic>\n                      strain Hetao-1 was isolated from\n                      <jats:italic>I. persulcatus<\/jats:italic>\n                      , and a draft genome sequence was obtained from the isolate. Sequencing determined the strain Hetao-1 genome to be approximately 906.1\u00a0kbp in length (28.9% average GC content), and MLSA identified the strain as ST633, which has previously been reported in Japan and Mongolia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      We detected\n                      <jats:italic>B. miyamotoi<\/jats:italic>\n                      from\n                      <jats:italic>I. persulcatus<\/jats:italic>\n                      ticks collected in Inner Mongolia, and successfully isolated a\n                      <jats:italic>B. miyamotoi<\/jats:italic>\n                      strain. To our knowledge, this is the first study to culture a\n                      <jats:italic>B. miyamotoi<\/jats:italic>\n                      isolate from China. The data on the prevalence of\n                      <jats:italic>B. miyamotoi<\/jats:italic>\n                      and other borreliae in\n                      <jats:italic>I. persulcatus<\/jats:italic>\n                      ticks will be fundamental for future epidemiological studies of\n                      <jats:italic>B. miyamotoi<\/jats:italic>\n                      disease in Inner Mongolia.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-021-04809-z","type":"journal-article","created":{"date-parts":[[2021,7,17]],"date-time":"2021-07-17T03:03:06Z","timestamp":1626490986000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Surveillance of Borrelia miyamotoi-carrying ticks and genomic analysis of isolates in Inner Mongolia, China"],"prefix":"10.1186","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7428-4101","authenticated-orcid":false,"family":"Gaowa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"family":"Wulantuya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kozue","family":"Sato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunhong","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuhong","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingfu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongxin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijing","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saixia","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zitong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minzhi","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guodong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunpu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dacheng","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norio","family":"Ohashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuji","family":"Ando","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Kawabata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,7,17]]},"reference":[{"key":"4809_CR1","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1093\/jmedent\/27.4.646","volume":"4","author":"TN Mather","year":"1990","unstructured":"Mather TN, Mather ME. 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