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Insecticide resistance is prevalent in\n                      <jats:italic>Ae<\/jats:italic>\n                      .\n                      <jats:italic>aegypti<\/jats:italic>\n                      around the world, and the resulting reduction of insecticide efficacy is likely to exacerbate the impact of dengue. Dengue has been a public health problem in Saudi Arabia, particularly in Jeddah, since its discovery there in the 1990s, and insecticide use for vector control is widespread throughout the city. An alternative approach to insecticide use, based on blocking dengue transmission in mosquitoes by the endosymbiont\n                      <jats:italic>Wolbachia<\/jats:italic>\n                      , is being trialed in Jeddah following the success of this approach in Australia and Malaysia. Knowledge of insecticide resistance status of mosquito populations in Jeddah is a prerequisite for establishing a\n                      <jats:italic>Wolbachia<\/jats:italic>\n                      -based dengue control program as releases of\n                      <jats:italic>Wolbachia<\/jats:italic>\n                      mosquitoes succeed when resistance status of the release population is similar to that of the wild population.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      WHO resistance bioassays of mosquitoes with deltamethrin, permethrin and DDT were used in conjunction with TaqMan\n                      <jats:sup>\u00ae<\/jats:sup>\n                      SNP Genotyping Assays to characterize mutation profiles of\n                      <jats:italic>Ae<\/jats:italic>\n                      .\n                      <jats:italic>aegypti<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Screening of the voltage-sensitive sodium channel (\n                      <jats:italic>V<\/jats:italic>\n                      ssc), the pyrethroid target site, revealed mutations at codons 989, 1016 and 1534 in\n                      <jats:italic>Ae<\/jats:italic>\n                      .\n                      <jats:italic>aegypti<\/jats:italic>\n                      from two districts of Jeddah. The triple mutant homozygote (1016G\/1534C\/989P) was confirmed from Al Safa and Al Rawabi. Bioassays with pyrethroids (Type I and II) and DDT showed that mosquitoes were resistant to each of these compounds based on WHO definitions. An association between\n                      <jats:italic>V<\/jats:italic>\n                      ssc mutations and resistance was established for the Type II pyrethroid, deltamethrin, with one genotype (989P\/1016G\/1534F) conferring a survival advantage over two others (989S\/1016V\/1534C and the triple heterozygote). An indication of synergism of Type I pyrethroid activity with piperonyl butoxide suggests that detoxification by cytochrome P450s accounts for some of the pyrethroid resistance response in\n                      <jats:italic>Ae<\/jats:italic>\n                      .\n                      <jats:italic>aegypti<\/jats:italic>\n                      populations from Jeddah.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The results provide a baseline for monitoring and management of resistance as well as knowledge of\n                      <jats:italic>V<\/jats:italic>\n                      ssc genotype frequencies required in\n                      <jats:italic>Wolbachia<\/jats:italic>\n                      release populations to ensure homogeneity with the target field population.\n                      <jats:italic>V<\/jats:italic>\n                      ssc mutation haplotypes observed show some similarity with those from\n                      <jats:italic>Ae<\/jats:italic>\n                      .\n                      <jats:italic>aegypti<\/jats:italic>\n                      in southeast Asia and the Indo-Pacific, but the presence of the triple mutant haplotype in three genotypes indicates that the species in this region may have a unique population history.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Graphical Abstract<\/jats:title>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-021-04867-3","type":"journal-article","created":{"date-parts":[[2021,7,11]],"date-time":"2021-07-11T23:10:35Z","timestamp":1626045035000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Voltage-sensitive sodium channel (Vssc) mutations associated with pyrethroid insecticide resistance in Aedes aegypti (L.) from two districts of Jeddah, Kingdom of Saudi Arabia: baseline information for a Wolbachia release program"],"prefix":"10.1186","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9834-4068","authenticated-orcid":false,"given":"Nancy M.","family":"Endersby-Harshman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AboElgasim","family":"Ali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basim","family":"Alhumrani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Abdullah","family":"Alkuriji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed B.","family":"Al-Fageeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulaziz","family":"Al-Malik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed S.","family":"Alsuabeyl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samia","family":"Elfekih","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ary A.","family":"Hoffmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,7,12]]},"reference":[{"issue":"2","key":"4867_CR1","doi-asserted-by":"publisher","first-page":"136","DOI":"10.1016\/j.pestbp.2012.06.003","volume":"104","author":"C Chang","year":"2012","unstructured":"Chang C, Huang X-Y, Chang P-C, Wu H-H, Dai S-M. 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