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DPP was administered topically, as spot-on, to four dogs on day 0. Hair from the eight dogs was collected individually by brushing the whole body on days 2, 7, 14, 21, 28 and 35. On each day of hair collection, 0.05\u00a0g of sampled hair was applied on the membrane corresponding to each feeding unit (FU). Seventy-two FU were each seeded with 30 adults of<jats:italic>I. scapularis<\/jats:italic>(<jats:italic>n<\/jats:italic>\u2009=\u200924 FU) or<jats:italic>I. ricinus<\/jats:italic>ticks (<jats:italic>n<\/jats:italic>\u2009=\u200948 FU). Bovine blood spiked with<jats:italic>B.<\/jats:italic>\u00a0<jats:italic>burgdorferi<\/jats:italic>sensu stricto (strain\u00a0B31) was added into each unit and changed every 12\u00a0h for 4 days. Tick mortality was assessed 1\u00a0h after seeding. One additional hour of incubation was added for live\/moribund specimens and reassessed for viability. All remaining live\/moribund ticks were left in the feeders and tick engorgement status was recorded at 96\u00a0h after seeding, and the uptake of<jats:italic>B. burgdorferi<\/jats:italic>s.s. was examined in the collected ticks by applying quantitative real-time PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Exposure to DPP-treated hair was 100% effective in blocking<jats:italic>B. burgdorferi<\/jats:italic>s.s. acquisition. The anti-feeding efficacy remained stable (100%) against both<jats:italic>Ixodes<\/jats:italic>species throughout the study. The acaricidal efficacy of DPP evaluated at 1 and 2\u00a0h after exposure was 100% throughout the study for<jats:italic>I. ricinus<\/jats:italic>, except the 1-h assessment on day 28 (95.9%) and day 35 (95.3%). The 1-h assessment of acaricidal efficacy was 100% at all time points for<jats:italic>I. scapularis<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The ex vivo feeding system developed here demonstrated a protective effect of DPP against the acquisition of<jats:italic>B. burgdorferi<\/jats:italic>without exposing the animals to the vectors or to the pathogen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Graphical Abstract<\/jats:title><\/jats:sec>","DOI":"10.1186\/s13071-021-04881-5","type":"journal-article","created":{"date-parts":[[2021,8,21]],"date-time":"2021-08-21T08:02:37Z","timestamp":1629532957000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Vectra 3D (dinotefuran, pyriproxyfen and permethrin) prevents acquisition of Borrelia burgdorferi sensu stricto by Ixodes ricinus and Ixodes scapularis ticks in an ex vivo feeding model"],"prefix":"10.1186","volume":"14","author":[{"given":"Djamel","family":"Tahir","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Btissam","family":"Asri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leon Nicolaas","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alec","family":"Evans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Mather","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Byron","family":"Blagburn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard K.","family":"Straubinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Choumet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frans","family":"Jongejan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1176-5802","authenticated-orcid":false,"given":"Marie","family":"Varloud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,21]]},"reference":[{"key":"4881_CR1","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1016\/j.cimid.2004.03.006","volume":"27","author":"P Parola","year":"2004","unstructured":"Parola P. 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