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It is caused by the trematode\n                      <jats:italic>Schistosoma mansoni<\/jats:italic>\n                      , which is transmitted by snails of the genus\n                      <jats:italic>Biomphalaria<\/jats:italic>\n                      . Among measures used to control and eliminate schistosomiasis, accurate mapping and monitoring of snail breeding sites are recommended. Despite the limitations of parasitological methods, they are still used to identify infected snails. Loop-mediated isothermal amplification (LAMP) is a sensitive, rapid, and cost-effective diagnostic method for the identification of infected snails. In the work reported here, we aimed to validate the use of LAMP for the detection of\n                      <jats:italic>S. mansoni<\/jats:italic>\n                      in snails of the genus\n                      <jats:italic>Biomphalaria<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Snails were collected in five municipalities of the Mucuri Valley and Jequitinhonha Valley regions in the state of Minas Gerais, Brazil. Snails were pooled according to collection site and then  squeezed for the detection of\n                      <jats:italic>S. mansoni<\/jats:italic>\n                      and other trematode larvae. Pooled snails were subjected to pepsin digestion and DNA extraction. Molecular assays were performed for species-specific identification and characterization of the samples. A previously described LAMP assay was adapted, evaluated, and validated using laboratory and field samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Using the parasitological method described here,\n                      <jats:italic>S. mansoni<\/jats:italic>\n                      cercariae were detected in snails from two collection sites, and cercariae of the family Spirorchiidae were found in snails from one site. The snails were identified by polymerase chain reaction (PCR)\u2013restriction fragment length polymorphism\u00a0(RFLP).\n                      <jats:italic>Biomphalaria glabrata<\/jats:italic>\n                      , the main snail host of\n                      <jats:italic>S. mansoni<\/jats:italic>\n                      in Brazil, was detected in 72.2% of the collection sites.\n                      <jats:italic>Biomphalaria kuhniana<\/jats:italic>\n                      , which is resistant to\n                      <jats:italic>S. mansoni<\/jats:italic>\n                      infection, was found in the remaining sites. Multiplex, low stringency (LS), and conventional PCR allowed the detection of positive snails in four additional sites. Trematodes belonging to the families Strigeidae and Echinostomatidae were detected by multiplex PCR in two sites. The LAMP assay was effective in detecting the presence of\n                      <jats:italic>S. mansoni<\/jats:italic>\n                      infection in laboratory (7\u00a0days post-infection) and field samples with no cross-reactivity for other trematodes. When compared to LS and conventional PCR, LAMP showed 100% specificity, 85.7% sensitivity, and a \u03ba index of 0.88.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Our findings suggest that LAMP is a good alternative method for the detection and monitoring of transmission foci of\n                      <jats:italic>S. mansoni<\/jats:italic>\n                      , as it was three times as effective as the parasitological examination  used here for the detection of infection, and is more directly applicable in the field than other molecular techniques.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Graphical abstract<\/jats:title>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-021-04888-y","type":"journal-article","created":{"date-parts":[[2021,8,6]],"date-time":"2021-08-06T03:03:23Z","timestamp":1628219003000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil"],"prefix":"10.1186","volume":"14","author":[{"given":"Silvia Gon\u00e7alves","family":"Mesquita","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Floria Gabriela dos Santos","family":"Neves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronaldo Guilherme Carvalho","family":"Scholte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar dos Santos","family":"Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina Toscano","family":"Fonseca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta Lima","family":"Caldeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,6]]},"reference":[{"key":"4888_CR1","unstructured":"World Health Organization. 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