{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T14:40:47Z","timestamp":1740148847191,"version":"3.37.3"},"reference-count":6,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T00:00:00Z","timestamp":1632182400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T00:00:00Z","timestamp":1632182400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We read with great interest the article by Yin et al. (Parasit Vectors 14:238, 2021). The authors found that <jats:italic>Cryptosporidium<\/jats:italic> infection induced significantly aberrant expression of circular RNA profiles in HCT-8 cells,  a finding which has far-reaching implications. However, due to the high number of false positives caused by multiple comparisons, statistical methods for microarray analysis should be carefully selected. Accurate analysis results will provide a convincing basis for subsequent experiments. In addition, we recommend several more appropriate methods in this article.<\/jats:p>","DOI":"10.1186\/s13071-021-04957-2","type":"journal-article","created":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T16:08:03Z","timestamp":1632240483000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Microarray analysis of circular RNAs in HCT-8 cells infected with Cryptosporidium parvum"],"prefix":"10.1186","volume":"14","author":[{"given":"Yuqing","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3333-8556","authenticated-orcid":false,"given":"Lei","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,9,21]]},"reference":[{"key":"4957_CR1","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1186\/s13071-020-04296-8","volume":"13","author":"SM Atoyebi","year":"2020","unstructured":"Atoyebi SM, Tchigossou GM, Akoton R, Riveron JM, Irving H, Weedall G, et al. Investigating the molecular basis of multiple insecticide resistance in a major malaria vector Anopheles funestus (sensu stricto) from Akaka-Remo, Ogun State, Nigeria. Parasit Vectors. 2020;13:423.","journal-title":"Parasit Vectors"},{"key":"4957_CR2","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1186\/s13071-021-04717-2","volume":"14","author":"JW Kim","year":"2021","unstructured":"Kim JW, Yi J, Park J, Jeong JH, Kim J, Won J, et al. Transcriptomic profiling of three-dimensional cholangiocyte spheroids long term exposed to repetitive Clonorchis sinensis excretory-secretory products. Parasit Vectors. 2021;14:213.","journal-title":"Parasit Vectors"},{"key":"4957_CR3","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1186\/s13071-021-04739-w","volume":"14","author":"YL Yin","year":"2021","unstructured":"Yin YL, Liu TL, Yao Q, Wang YX, Wu XM, Wang XT, et al. Circular RNA ciRS-7 affects the propagation of Cryptosporidium parvum in HCT-8 cells by sponging miR-1270 to activate the NF-\u03baB signaling pathway. Parasit Vectors. 2021;14:238.","journal-title":"Parasit Vectors"},{"key":"4957_CR4","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1186\/s13059-018-1466-5","volume":"19","author":"AT Assefa","year":"2018","unstructured":"Assefa AT, De Paepe K, Everaert C, Mestdagh P, Thas O, Vandesompele J. Differential gene expression analysis tools exhibit substandard performance for long non-coding RNA-sequencing data. Genome Biol. 2018;19:96.","journal-title":"Genome Biol"},{"key":"4957_CR5","doi-asserted-by":"publisher","first-page":"1408","DOI":"10.12688\/f1000research.9005.1","volume":"5","author":"CW Law","year":"2016","unstructured":"Law CW, Alhamdoosh M, Su S, Dong X, Tian L, Smyth GK, et al. RNA-seq analysis is easy as 1-2-3 with limma, Glimma and edgeR. 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Nucleic Acids Res. 2015;43: e47.","journal-title":"Nucleic Acids Res"}],"container-title":["Parasites &amp; Vectors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-021-04957-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13071-021-04957-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-021-04957-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T16:11:49Z","timestamp":1632240709000},"score":1,"resource":{"primary":{"URL":"https:\/\/parasitesandvectors.biomedcentral.com\/articles\/10.1186\/s13071-021-04957-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,21]]},"references-count":6,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["4957"],"URL":"https:\/\/doi.org\/10.1186\/s13071-021-04957-2","relation":{},"ISSN":["1756-3305"],"issn-type":[{"type":"electronic","value":"1756-3305"}],"subject":[],"published":{"date-parts":[[2021,9,21]]},"assertion":[{"value":"14 July 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 August 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 September 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"485"}}