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Maintained in nature by <jats:italic>Ixodes<\/jats:italic> spp. ticks, POWV has also been isolated from species of other hard tick genera, yet it is unclear if these species can serve as vectors. <jats:italic>Dermacentor variabilis<\/jats:italic> and <jats:italic>Amblyomma americanum<\/jats:italic> share geographic and ecologic overlap with <jats:italic>Ixodes<\/jats:italic> spp. ticks and POWV transmission foci, raising the possibility that POWV could become established in these tick species and leading to range expansion and increased human risk. Therefore, we assessed the competency of <jats:italic>Ixodes scapularis<\/jats:italic>, <jats:italic>D. variabilis<\/jats:italic> and <jats:italic>A. americanum<\/jats:italic> for POWV lineage II (POWV II).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Larvae from all three species were co-infested on POWV-infected Balb\/c mice. The engorged larvae were allowed to molt to nymphs and screened for the presence of POWV II RNA by reverse transcription-qPCR. Eight infected nymphs from each species were allowed to individually feed on a na\u00efve mouse. Mice were screened for the presence of POWV II RNA to determine infection status.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The results demonstrated that larvae from all three tick species were able to efficiently acquire POWV II via feeding on viremic mice, maintain infection through molting and successively transmit POWV to na\u00efve mice at the nymphal stage at comparable rates across all three species.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Our findings reveal that non-<jats:italic>Ixodes<\/jats:italic> tick species can serve as competent vectors for POWV and highlight the potential role of these species in the ecology and epidemiology of POWV. Future studies examining the possible implications of these findings on POWV epidemiology and the adaptability of POWV in these new vectors are warranted.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-021-04974-1","type":"journal-article","created":{"date-parts":[[2021,9,9]],"date-time":"2021-09-09T09:02:47Z","timestamp":1631178167000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Vector competence of human-biting ticks Ixodes scapularis, Amblyomma americanum and Dermacentor variabilis for Powassan virus"],"prefix":"10.1186","volume":"14","author":[{"given":"Rohit","family":"Sharma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duncan W.","family":"Cozens","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip M.","family":"Armstrong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas E.","family":"Brackney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,9,9]]},"reference":[{"issue":"6","key":"4974_CR1","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1111\/zph.12485","volume":"65","author":"T Corrin","year":"2018","unstructured":"Corrin T, Greig J, Harding S, Young I, Mascarenhas M, Waddell LA. 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