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A survey conducted at that time was unsuccessful in implicating any mosquito species as the possible vector. Although active anopheline mosquito surveillance continued until the middle of the 1980s, there is very limited information on their current status and distribution in Japan. Therefore, this study is an update on the current status and distribution of anopheline mosquitoes in Hokkaido based on a 15-year entomological surveillance between 2001 and 2015.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>A survey of mosquitoes was conducted at 22 sites in Hokkaido, Japan, from 2001 to 2015. Adult mosquitoes were collected from cowsheds, lakesides, shrubs, and habitats ranging from open grassland to coniferous forest using a Centers for Disease Control and Prevention (CDC) miniature light trap enhanced with dry ice, aspirators, and sweeping nets. Larvae were collected from lakes, ponds, swamps, stagnant and flowing rivers, and paddy fields. All specimens were morphologically identified and subjected to polymerase chain reaction (PCR)-based sequence analysis of the internal transcribed spacer 2 ( ITS2) region of rDNA. Phylogenetic trees were reconstructed using the neighbor-joining method with the Kimura 2-parameter model on MEGA X version 10.2.2.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>A total of 46 anopheline specimens were used for the phylogenetic analysis. During the survey, a new member of the <jats:italic>Anopheles hyrcanus<\/jats:italic> group, <jats:italic>An. belenrae<\/jats:italic>, was discovered in eastern Hokkaido in 2004. <jats:italic>Anopheles belenrae<\/jats:italic> has since then been consistently found and confirmed to inhabit only this area of Japan. Four members of the <jats:italic>An. hyrcanus<\/jats:italic> group, namely <jats:italic>An. belenrae<\/jats:italic>, <jats:italic>An. engarensis<\/jats:italic>, <jats:italic>An. lesteri<\/jats:italic>, and <jats:italic>An. sineroides<\/jats:italic>, have been found in Hokkaido. The results also suggest that <jats:italic>An. sinensis<\/jats:italic>, formerly a dominant species throughout Japan, has become a rarely found species, at least currently in Hokkaido.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>The updated distribution of anopheline mosquitoes in Hokkaido, Japan, showed considerable differences from that observed in previous surveys conducted from 1969 to 1984. In particular, areas where <jats:italic>An. sinensis<\/jats:italic> was previously distributed may have been greatly reduced in Hokkaido. The phylogenetic analysis revealed a novel <jats:italic>An. hyrcanus<\/jats:italic> group member identified as <jats:italic>An. belenrae<\/jats:italic>, described in South Korea in 2005. It is interesting that <jats:italic>An. belenrae<\/jats:italic> was confirmed to inhabit only eastern Hokkaido, Japan.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-021-04995-w","type":"journal-article","created":{"date-parts":[[2021,9,26]],"date-time":"2021-09-26T14:06:30Z","timestamp":1632665190000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Updated distribution of anopheline mosquitoes in Hokkaido, Japan, and the first evidence of Anopheles belenrae in Japan"],"prefix":"10.1186","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6877-2010","authenticated-orcid":false,"given":"Kyoko","family":"Sawabe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nozomi","family":"Imanishi-Kobayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihide","family":"Maekawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukiko","family":"Higa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyeong Soon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keita","family":"Hoshino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshio","family":"Tsuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihiko","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoko","family":"Nihei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Takai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Kurihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mutsuo","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,9,26]]},"reference":[{"key":"4995_CR1","first-page":"340","volume":"6","author":"M Sasa","year":"1949","unstructured":"Sasa M, Takahashi H, Kohgo T, Oshima H. 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