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There have been reports of cases of acute or chronic cases of Chagas disease among active military personnel. These may be the result of military-associated activities performed in jungles and other endemic areas or the consequence of exposure to <jats:italic>Trypanosoma cruzi<\/jats:italic> inside military establishments\/facilities located in endemic areas. The aim of the present study was to describe the circulation of <jats:italic>T. cruzi<\/jats:italic> inside facilities housing four training and re-training battalions [Battalions of Instruction, Training en Re-training (BITERs)] located in municipalities with historical reports of triatomine bugs and Chagas disease cases. An entomological and faunal survey of domestic and sylvatic environments was conducted inside each of these military facilities.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Infection in working and stray dogs present in each BITER location was determined using serological and molecular tools, and <jats:italic>T. cruzi<\/jats:italic> in mammal and triatomine bug samples was determined by PCR assay. The PCR products of the vertebrate <jats:italic>12S rRNA<\/jats:italic> gene were also obtained and subjected to Sanger sequencing to identify blood-feeding sources. Finally, we performed a geospatial analysis to evaluate the coexistence of infected triatomines and mammals with the military personal inside of each BITER installation.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>In total, 86 specimens were collected: 82 <jats:italic>Rhodnius pallescens<\/jats:italic>, two <jats:italic>Rhodnius prolixus<\/jats:italic>, one <jats:italic>Triatoma dimidiata<\/jats:italic> and one <jats:italic>Triatoma maculata.<\/jats:italic> The overall <jats:italic>T. cruzi<\/jats:italic> infection rate for <jats:italic>R. pallescens<\/jats:italic> and <jats:italic>R. prolixus<\/jats:italic> was 56.1 and 100% respectively, while <jats:italic>T. dimidiata<\/jats:italic> and <jats:italic>T. maculata<\/jats:italic> were not infected. Eight feeding sources were found for the infected triatomines, with opossum and humans being the most frequent sources of feeding\u00a0(85.7%). Infection was most common in the common opossum <jats:italic>Didelphis marsupialis<\/jats:italic>, with infection levels of 77.7%. Sylvatic TcI was the most frequent genotype, found in 80% of triatomines and 75% of <jats:italic>D. marsupialis.<\/jats:italic> Of the samples collected from dogs (<jats:italic>n<\/jats:italic> = 52), five (9.6%; 95% confidence interval: 3.20\u201321.03) were seropositive based on two independent tests. Four of these dogs were creole and one was a working dog. The spatial analysis revealed a sympatry between infected vectors and mammals with the military population.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>We have shown a potential risk of spillover of sylvatic <jats:italic>T. cruzi<\/jats:italic> transmission to humans by oral and vectorial transmission in two BITER installations in Colombia. The results indicate  that installations where 100,000 active military personnel carry out training activities should be prioritized for epidemiological surveillance of Chagas disease.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-021-05018-4","type":"journal-article","created":{"date-parts":[[2021,10,10]],"date-time":"2021-10-10T18:07:46Z","timestamp":1633889266000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The potential risk of enzootic Trypanosoma cruzi transmission inside four training and re-training military battalions (BITER) in Colombia"],"prefix":"10.1186","volume":"14","author":[{"given":"Omar","family":"Cantillo-Barraza","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffer","family":"Torres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanira","family":"Romero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Zuluaga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camilo A.","family":"Correa-C\u00e1rdenas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanny","family":"Herrera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omaira","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Teresa","family":"Alvarado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan David","family":"Ram\u00edrez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9280-2531","authenticated-orcid":false,"given":"Claudia","family":"M\u00e9ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,10,9]]},"reference":[{"key":"5018_CR1","unstructured":"World Health Organization. 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