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In humans, the clinical manifestations of Lyme borreliosis vary from dermatological infection to severe systemic manifestations. In Romania, data on the seroprevalence of Lyme borreliosis and associated risk factors are scarce and outdated, as the only seroprevalence study with a large dataset was published more than 20\u00a0years ago. Therefore, the aim of the present study was to evaluate the seroprevalence for <jats:italic>Borrelia burgdorferi<\/jats:italic> s.l. in healthy blood donors from six Romanian counties and identify the associated risk factors.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>The study was conducted among 1200 healthy blood donors aged between 18 and 65\u00a0years during November 2019 and September 2020 from six counties in the northwestern and central parts of Romania. A two-tiered testing strategy was applied. Positive and equivocal immunoenzymatic test results for IgG and IgM antibodies were further confirmed by Western blot.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Serum samples from 20% of the blood donors had positive or equivocal IgG and IgM ELISA index values. In total, 2.3% of the serum samples for IgG and 1.8% for IgM were positive by Western blot. The seroprevalence for both antibodies varied between 1.5% (Satu-Mare) and 6.5% (Bistri\u021ba-N\u0103s\u0103ud) in the six counties investigated. The highest seroprevalence was observed in men (4.7%), in blood donors performing their professional activities outdoors (4.2%), and in those aged\u2009 \u2265\u200956 years (8%).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>These findings confirm the presence of specific IgG and IgM antibodies to <jats:italic>B. burgdorferi<\/jats:italic> s.l. among healthy blood donors from Romania. Furthermore, potential risk factors, such as gender, age, and behavior, associated with the presence of positive <jats:italic>B. burgdorferi<\/jats:italic> s.l. antibodies among healthy blood donors were identified.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-021-05099-1","type":"journal-article","created":{"date-parts":[[2021,12,4]],"date-time":"2021-12-04T18:02:27Z","timestamp":1638640947000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Seroprevalence of antibodies against Borrelia burgdorferi sensu lato in healthy blood donors in Romania: an update"],"prefix":"10.1186","volume":"14","author":[{"given":"Zsuzsa","family":"Kalm\u00e1r","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Violeta","family":"Briciu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mircea","family":"Coroian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirela","family":"Flonta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda-Lelia","family":"R\u0103dulescu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Topan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrei Daniel","family":"Mihalca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihaela","family":"Lup\u0219e","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,12,4]]},"reference":[{"key":"5099_CR1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12942-018-0163-7","volume":"17","author":"A Estrada-Pe\u00f1a","year":"2018","unstructured":"Estrada-Pe\u00f1a A, Cutler S, Potkonjak A, Vassier-Tussaut M, Bortel W, Zeller H, et al. 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