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The aim of this study was to identify the composition of questing tick species and the associated pathogens at different sites near the German Baltic coast.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Questing ticks were collected monthly at four sites (May\u2013November, 2020), mainly grasslands, and in October and November 2020 at a fifth site. Molecular screening of ticks for pathogens included RT-qPCR for the tick-borne encephalitis virus (TBEV), qPCR for <jats:italic>Anaplasma phagocytophilum,<\/jats:italic> PCR for <jats:italic>Babesia<\/jats:italic> species and <jats:italic>Rickettsia<\/jats:italic> species, and nested PCR for <jats:italic>Borrelia<\/jats:italic> species.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Altogether 1174 questing ticks were collected: 760 <jats:italic>Ixodes ricinus<\/jats:italic>, 326 <jats:italic>D. reticulatus<\/jats:italic> and 88 <jats:italic>Haemaphysalis concinna<\/jats:italic>. The highest activity peak of <jats:italic>I. ricinus<\/jats:italic> and <jats:italic>D. reticulatus<\/jats:italic> was in May, in June for <jats:italic>H. concinna<\/jats:italic> while a second peak was observed only for <jats:italic>I. ricinus<\/jats:italic> and <jats:italic>D. reticulatus<\/jats:italic> in September and October, respectively. All samples tested negative for TBEV. For <jats:italic>A. phagocytophilum<\/jats:italic>, 1.5% of <jats:italic>I. ricinus<\/jats:italic> adults tested positive while the minimum infection rate (MIR) in nymphs was 1.3%. This pathogen was found in 0.6% of <jats:italic>D. reticulatus<\/jats:italic>. <jats:italic>Babesia<\/jats:italic> spp. were detected in <jats:italic>I. ricinus<\/jats:italic> (18.2% adults, 2.1% MIR in nymphs) and <jats:italic>H. concinna<\/jats:italic> (13.3% adults, 9.7% MIR in nymphs). <jats:italic>Borrelia<\/jats:italic> spp. were present only in <jats:italic>I. ricinus<\/jats:italic> (49.1% adults, 11.9% MIR in nymphs), while <jats:italic>Rickettsia<\/jats:italic> spp. were detected in <jats:italic>I. ricinus<\/jats:italic> (14% adults, 8.9% MIR in nymphs) and <jats:italic>D. reticulatus<\/jats:italic> (82%). Co-detection of pathogens was observed in 26.6% and 54.8% of positive <jats:italic>I. ricinus<\/jats:italic> adults and nymph pools, respectively, while one <jats:italic>D. reticulatus<\/jats:italic> tested positive for <jats:italic>A. phagocytophilum<\/jats:italic> and <jats:italic>Rickettsia<\/jats:italic> spp. The most common co-infection in <jats:italic>I. ricinus<\/jats:italic> adults was <jats:italic>Babesia microti<\/jats:italic> and <jats:italic>Borrelia afzelii<\/jats:italic> (12.3% of positive ticks).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The results of this study confirm the northern expansion of <jats:italic>D. reticulatus<\/jats:italic> and <jats:italic>H. concinna<\/jats:italic> in Germany. The detailed data of the infection levels at each location could be useful in assessing the risk of pathogen acquisition following a tick bite.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-022-05173-2","type":"journal-article","created":{"date-parts":[[2022,2,22]],"date-time":"2022-02-22T11:03:19Z","timestamp":1645527799000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Sympatric occurrence of Ixodes ricinus with Dermacentor reticulatus and Haemaphysalis concinna and the associated tick-borne pathogens near the German Baltic coast"],"prefix":"10.1186","volume":"15","author":[{"given":"Cristian","family":"R\u0103ileanu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Tauchmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia","family":"Silaghi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,2,22]]},"reference":[{"issue":"1","key":"5173_CR1","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1007\/s10493-021-00619-1","volume":"84","author":"F Rubel","year":"2021","unstructured":"Rubel F, Brugger K, Chitimia-Dobler L, Dautel H, Meyer-Kayser E, Kahl O. 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