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Epidemiological studies that measure both infection and potential risk factors associated with infection help to understand the drivers of transmission in a population and therefore can provide information to optimise STH control programmes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>During October and November 2019, we conducted a cross-sectional survey of the prevalence and intensity of STH infection and associated risk factors among 7710 primary-school-age children from 64 primary schools across 13 districts in Dak Lak province, Vietnam. Quantitative PCR (qPCR) was used to detect and quantify STH infections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The predominant STH species was the hookworm<jats:italic>Necator americanus<\/jats:italic>(overall cluster-adjusted prevalence of 13.7%), and its prevalence was heterogeneously distributed across surveyed schools (0% to 56.3%). All other STH species had a prevalence of less than 1%. Using mixed-effects logistic regression, we found that the adjusted odds ratio (aOR) was significantly higher for both infection and moderate-to-heavy-intensity infection with<jats:italic>N. americanus<\/jats:italic>among children from multiple ethnic minority groups, compared to children from the majority group (Kinh). Adjusted odds of infection with<jats:italic>N. americanus<\/jats:italic>were also higher in children who reported practising open defecation at school (aOR 1.42, 95% CI 1.05, 1.93,<jats:italic>P<\/jats:italic>\u2009=\u20090.02) and in those who had an unimproved household water supply (aOR 1.28, 95% CI 1.04, 1.57,<jats:italic>P<\/jats:italic>\u2009=\u20090.02). Conversely, children with a flushing household toilet had a reduced risk of infection (aOR 0.58, 95% CI 0.47, 0.70,<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), as did those whose primary female carer attended secondary (aOR 0.65, 95% CI 0.51, 0.84,<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) or tertiary education (aOR 0.39, 95% CI 0.24, 0.63,<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study is the largest reported prevalence survey of STH infections conducted using qPCR as a diagnostic technique. The findings of higher adjusted odds of infection amongst ethnic minority children highlight that STH control programmes may not be reaching certain population groups and that additional culturally appropriate approaches may be required. Additionally, the associations between specific WASH factors and infection indicate potential programmatic targets to complement preventive chemotherapy programmes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Graphical Abstract<\/jats:title><\/jats:sec>","DOI":"10.1186\/s13071-023-05809-x","type":"journal-article","created":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T13:03:01Z","timestamp":1687870981000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Epidemiology of soil-transmitted helminths using quantitative PCR and risk factors for hookworm and Necator americanus infection in school children in Dak Lak province, Vietnam"],"prefix":"10.1186","volume":"16","author":[{"given":"Angus","family":"Hughes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinh","family":"Ng-Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi E.","family":"Clarke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clare E. 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