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The efficacy of Simparica Trio<jats:sup>\u00ae<\/jats:sup> (Zoetis) in the prevention of <jats:italic>B. canis<\/jats:italic> transmission was evaluated at the minimum recommended label dose of 1.2\u00a0mg\/kg sarolaner, 24\u00a0\u00b5g\/kg moxidectin and 5\u00a0mg\/kg pyrantel per kg bodyweight.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Twenty-four (24) dogs were randomly allocated to either a placebo-treated group or one of two treatment groups with Simparica Trio. Dogs were infested with <jats:italic>B. canis<\/jats:italic>-infected <jats:italic>Dermacentor reticulatus<\/jats:italic> ticks 21 or 28\u00a0days after treatment administration. Blood samples for antibody and DNA detection were collected from each dog prior to tick infestation until 28\u00a0days after infestation. A dog was defined as being <jats:italic>B. canis<\/jats:italic> positive if it tested positive by both an indirect immunofluorescence assay (IFA) and PCR at any time during the study.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>No treatment-related adverse reactions were recorded during the study. All placebo-treated animals displayed clinical signs due to babesiosis and tested positive on both IFA and PCR. None of the Simparica Trio-treated animals displayed any clinical symptoms or tested positive, resulting in a 100% efficacy in the prevention of canine babesiosis (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>A single treatment with Simparica Trio at the minimum recommended label dose of 1.2\u00a0mg\/kg sarolaner, 24\u00a0\u00b5g\/kg moxidectin and 5\u00a0mg\/kg pyrantel per kg bodyweight prevents the transmission of <jats:italic>B. canis<\/jats:italic> by infected <jats:italic>D. reticulatus<\/jats:italic> to dogs for at least 28\u00a0days.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-023-06115-2","type":"journal-article","created":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T06:02:24Z","timestamp":1706853744000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of the efficacy of Simparica Trio\u00ae in the prevention of the transmission of Babesia canis by infected Dermacentor reticulatus to dogs"],"prefix":"10.1186","volume":"17","author":[{"given":"Stasia","family":"Borowski","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alta","family":"Viljoen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"D\u2019Hanis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean","family":"Mahabir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Geurden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,2,2]]},"reference":[{"key":"6115_CR1","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1016\/j.vetpar.2009.03.028","volume":"163","author":"F Beugnet","year":"2009","unstructured":"Beugnet F, Mari\u00e9 JL. 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