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The gold immunochromatographic assay (GICA) is a means of rapidly detecting pathogen in samples. GICA-based diagnostic methods have been developed to accurately detect pathogens with high sensitivity and specificity, and their application in <jats:italic>T. gondii<\/jats:italic> diagnosis is expected to yield good results.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Colloidal gold test strips were produced using <jats:italic>T. gondii<\/jats:italic> C-terminal truncated apical membrane antigen 1 (AMA1C). Colloidal gold-AMA1C and colloidal gold-murine protein conjugate were synthesized under optimal conditions. A nitrocellulose membrane was treated with AMA1C and goat anti-mouse antibody as the test line and control line, respectively. In total, 90 cat serum samples were tested using AMA1C-GICA and a commercial enzyme linked immunosorbent assay (ELISA) kit. The GICA results were digitally displayed using a portable colloidal gold immunochromatographic test strip analyzer (HMREADER). The sensitivity, specificity, and stability of AMA1C-GICA were assessed, and this was then used to examine clinical samples, including 203 human sera, 266 cat sera, and 81 dog sera.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>AMA1C-GICA had a detection threshold of 1:32 for <jats:italic>T. gondii<\/jats:italic>-positive serum. The GICA strips specifically detected <jats:italic>T. gondii<\/jats:italic> antibodies and exhibited no reactivity with <jats:italic>Plasmodium vivax<\/jats:italic>, <jats:italic>Paragonimus kellicotti<\/jats:italic>, <jats:italic>Schistosoma japonicum<\/jats:italic>, <jats:italic>Clonorchis sinensis<\/jats:italic>, and <jats:italic>Schistosoma mansoni<\/jats:italic>. Consequently, 15 (16.7%) positive samples were detected using the AMA1C-GICA and commercial ELISA kits for each of the assays. The receiver-operating characteristic curve showed that GICA had a relative sensitivity of 85.3% and specificity of 92%, with an area under the curve of 98%. After analyzing clinical samples using HMREADER, 1.2%\u201323.4% of these samples were found to be positive for <jats:italic>T. gondii<\/jats:italic>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>This study presents a novel assay that enables timely and efficient detection of serum antibodies against <jats:italic>T. gondii<\/jats:italic>, thereby allowing for its early clinical diagnosis. Furthermore, the integration of digital detection using HMREADER can enhance the implementation of GICA.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-024-06180-1","type":"journal-article","created":{"date-parts":[[2024,2,22]],"date-time":"2024-02-22T08:04:43Z","timestamp":1708589083000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Application of gold immunochromatographic assay strip combined with digital evaluation for early detection of Toxoplasma gondii infection in multiple species"],"prefix":"10.1186","volume":"17","author":[{"given":"Jiyuan","family":"Fan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawen","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yafan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haojie","family":"Ding","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingming","family":"Kong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xunhui","family":"Zhuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaohong","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,2,22]]},"reference":[{"key":"6180_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1863-2378.2009.01276.x","volume":"57","author":"EA Innes","year":"2010","unstructured":"Innes EA. 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Four female New Zealand rabbits (2\u20132.5\u00a0kg) were kept in the experimental animal house at Center of Zhejiang Academy of Medical Sciences, Zhejiang Province, China, and all steps of operation for animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of Hangzhou Medical College (Zhejiang Academy of Medical Sciences) (Approval File No. 2021-010). Additionally, all rabbits were allowed for experimentation by the breeders. Samples from cats and dogs were approved by the Hangzhou Medical College Institutional Animal Care and Use Committee (Approval File No: 2023-038) and followed the Chinese legislation about the use and care of research animals (GB\/T35823-2018). All methods were performed in accordance with the relevant guidelines and regulations and conducted as per the Animal Research: Reporting in Vivo Experiments (ARRIVE) Guidelines.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"81"}}