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Dogs are considered important reservoirs of <jats:italic>T.\u00a0penetrans<\/jats:italic> in Brazil. The aim of this study was to determine the monthly insecticidal efficacy of a single oral administration of fluralaner at a dose of 10\u201318\u00a0mg\/kg (Bravecto<jats:sup>\u00ae<\/jats:sup> 1-Month, also registered as Defenza<jats:sup>\u00ae<\/jats:sup> in some countries; MSD Animal Health) in dogs naturally infested with <jats:italic>T. penetrans<\/jats:italic>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>This clinical trial was conducted in a rural community located in Ilh\u00e9us, Bahia, Brazil. A total of 64 dogs were selected and distributed in a completely randomized design between a treated group (TG) that received one single dose of Bravecto<jats:sup>\u00ae<\/jats:sup> 1-Month (Defenza<jats:sup>\u00ae<\/jats:sup>) and a negative control group (CG) that received no treatment. Each group was composed of 32 dogs. The evaluations took place on days 0, 7\u2009\u00b1\u20092, 14\u2009\u00b1\u20092, 21\u2009\u00b1\u20092, 28\u2009\u00b1\u20092, 35\u2009\u00b1\u20092, and 42\u2009\u00b1\u20092 post treatment, in which the dogs were inspected to evaluate the infestation stage and classify lesions associated with tungiasis. The primary efficacy was determined from the percentage of treated dogs free of fleas (stage II and III lesions) after administration of the formulation at each evaluation time. Secondary efficacy was based on the number of active lesions (stages II and III) in each group at each evaluation time. The clinical condition of the animals was defined based on the Severity Score for Acute Dog Tungiasis (SCADT), which is related to the number and severity of lesions.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The primary efficacy of the product was greater than 95.0% from days 7 to 21 and reached 100.0% between days 28 and 42, with a significant association between treatment and infestation decline (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.025) between days 7 and 42. Secondary drug efficacy was greater than 99.9% from days 7 to 21, reaching 100.0% between days 28 and 42 (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). The treated dogs also scored lower on the SCADT than the control animals did during the entire clinical evaluation period (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>A single administration of Bravecto<jats:sup>\u00ae<\/jats:sup> 1-Month (Defenza<jats:sup>\u00ae<\/jats:sup>) was effective in eliminating <jats:italic>Tunga penetrans<\/jats:italic> infestations, as well as in preventing parasitism for at least 42\u00a0days after treatment.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-024-06272-y","type":"journal-article","created":{"date-parts":[[2024,4,29]],"date-time":"2024-04-29T17:01:51Z","timestamp":1714410111000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Efficacy of monthly treatment with oral fluralaner (Bravecto\u00ae 1-Month) against Tunga penetrans in dogs in Brazil: a randomized, double-blind, controlled field study"],"prefix":"10.1186","volume":"17","author":[{"given":"Katharine Costa","family":"dos Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula Elisa Brand\u00e3o","family":"Guedes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George R\u00eago","family":"Albuquerque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anderson Vieira","family":"de Jesus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anai\u00e1","family":"da Paix\u00e3o Sev\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana Thaisa Santos","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamille Bispo","family":"de Carvalho Teixeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thammy Vieira","family":"Bitar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiani Vitor","family":"Harvey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia Nadir Sanches","family":"Ramos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Bonomi","family":"Barufi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"de Almeida Borges","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata Santiago Alberto","family":"Carlos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,29]]},"reference":[{"key":"6272_CR1","volume-title":"A Hot Topics in Infection and Immunity in Children","author":"P Hotez","year":"2006","unstructured":"Hotez P, Ottesen E, Fenwick A, Molyneux D. 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This study was conducted considering the principles of \u201cGood Clinical Practice\u201d VICH GL9 (GCP) adopted by the Committee for Medicinal Products for Veterinary Use (CVMP), European Medicines Agency (EMA) in June 2000 (CVMP\/VICH\/595\/98-Final). Informed consent was obtained from the owners of all animals included in the study.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The following authors have a conflict of interest: Sofia Nadir Sanches Ramos and Francisco Bonomi Barufi are employees at MSD Animal Health. The other authors declare no conflicts of interest.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"197"}}