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Hunan Province ranks 12th in the production of beef and mutton in China. However, limited data are available on the seroprevalence, risk factors and molecular characterization of <jats:italic>T. gondii<\/jats:italic> and <jats:italic>N. caninum<\/jats:italic> in beef cattle and goats in Hunan province, China.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Sera of 985 beef cattle and 1147 goats were examined for the presence of specific antibodies against <jats:italic>T. gondii<\/jats:italic> using indirect hemagglutination test (IHAT) and anti-<jats:italic>N. caninum<\/jats:italic> IgG using competitive-inhibition enzyme-linked immunoassay assay (cELISA). Statistical analysis of possible risk factors was performed using PASW Statistics. Muscle samples of 160 beef cattle and 160 goats were examined for the presence of <jats:italic>T. gondii<\/jats:italic> DNA (B1 gene) and <jats:italic>N. caninum<\/jats:italic> DNA (Nc-5 gene) by nested PCR. The B1 gene-positive samples were genotyped at 10 genetic markers using the multilocus nested PCR-RFLP (Mn-PCR-RFLP).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Specific IgG against <jats:italic>T. gondii<\/jats:italic> were detected in 8.3% (82\/985) and 13.3% (153\/1147) and against <jats:italic>N. caninum<\/jats:italic> in 2.1% (21\/985) and 2.0% (23\/1147) of the beef cattle and goats, respectively. Based on statistical analysis, the presence of cats, semi-intensive management mode and gender were identified as significant risk factors for <jats:italic>T. gondii<\/jats:italic> infection in beef cattle. Age was a significant risk factor for <jats:italic>T. gondii<\/jats:italic> infection in goats (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05), and age &gt; 3\u00a0years was a significant risk factor for <jats:italic>N. caninum<\/jats:italic> infection in beef cattle (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). PCR positivity for <jats:italic>T. gondii<\/jats:italic> was observed in three beef samples (1.9%; 3\/160) and seven chevon samples (4.4%; 7\/160). Genotyping of PCR positive samples identified one to be ToxoDB#10. The <jats:italic>N. caninum<\/jats:italic> DNA was observed in one beef sample (0.6%; 1\/160) but was negative in all chevon samples.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>To our knowledge, this is the first large-scale serological and molecular investigation of <jats:italic>T. gondii<\/jats:italic> and <jats:italic>N. caninum<\/jats:italic> and assessment of related risk factors in beef cattle and goats in Hunan Province, China. The findings provide baseline data for executing prevention and control of these two important parasites in beef cattle and goats in China.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-024-06283-9","type":"journal-article","created":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T19:02:09Z","timestamp":1714158129000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Seroprevalence and molecular detection of Toxoplasma gondii and Neospora caninum in beef cattle and goats in Hunan province, China"],"prefix":"10.1186","volume":"17","author":[{"given":"Xi-Long","family":"Yi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-He","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He-Liang","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei-Ling","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Cheng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Jing","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing-Quan","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo-Hua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,4,26]]},"reference":[{"key":"6283_CR1","doi-asserted-by":"publisher","first-page":"455","DOI":"10.3389\/fvets.2020.00455","volume":"7","author":"LD De Barros","year":"2020","unstructured":"De Barros LD, Garcia JL, Bresciani KDS, Cardim ST, Storte VS, Headley SA. 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