{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T23:06:14Z","timestamp":1779145574110,"version":"3.51.4"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T00:00:00Z","timestamp":1720483200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T00:00:00Z","timestamp":1720483200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Angiostrongyliasis is a zoonotic parasitic disease caused by the rat lungworm <jats:italic>Angiostrongylus cantonensis<\/jats:italic>. The intermediate hosts of <jats:italic>A. cantonensis<\/jats:italic> are gastropods, and snail species such as <jats:italic>Pomacea canaliculata<\/jats:italic> play a key role in the transmission of human angiostrongyliasis. Detecting <jats:italic>A. cantonensis<\/jats:italic> infection in snails is an important component of epidemiological surveillance and the control of angiostrongyliasis.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>In this study, a new method for diagnosing <jats:italic>A. cantonensis<\/jats:italic> infection in gastropods was developed by recovering larvae from the buccal cavity of three snail species. The entire buccal cavity of a snail was extracted, and the tissue was pressed between two microscope slides to observe whether <jats:italic>A. cantonensis<\/jats:italic> larvae were present. Our new method was compared with traditional pathogenic detection methods of lung microscopy, tissue homogenization, and artificial digestion. We artificially infected 160 <jats:italic>P. canaliculata<\/jats:italic>, 160 <jats:italic>Cipangopaludina chinensis<\/jats:italic>, and 160 <jats:italic>Bellamya aeruginosa<\/jats:italic> snails with <jats:italic>A. cantonensis<\/jats:italic>. Then, the four different detection methods were used to diagnose infection in each snail species at 7, 14, 21, and 28\u00a0days post exposure.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>We found no significant difference in the percentages of infected <jats:italic>P. canaliculata<\/jats:italic> snails using the four methods to detect <jats:italic>A. cantonensis<\/jats:italic> larvae. The radula pressing method had a mean detection rate of 80%, while the lung microscopy (81.3%), tissue homogenization (83.8%), and artificial digestion (85%) methods had slightly greater detection rates. Similarly, the percentages of infected <jats:italic>C. chinensis<\/jats:italic> snails that were detected using the radula pressing (80%), tissue homogenization (82.1%), and artificial digestion (83.8%) methods were not significantly different. Finally, the percentages of infected <jats:italic>B. aeruginosa<\/jats:italic> snails that were detected using the radula pressing (81.3%), tissue homogenization (81.9%), and artificial digestion (81.4%) methods were not significantly different. These results showed that the radula pressing method had a similar detection rate to traditional lung microscopy, tissue homogenization, or artificial digestion methods.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>This study demonstrates a new method for the qualitative screening of gastropods that act as intermediate hosts of <jats:italic>A. cantonensis<\/jats:italic> (and other <jats:italic>Angiostrongylus<\/jats:italic> species), provides technical support for the control of human angiostrongyliasis, and furthers research on <jats:italic>A. cantonensis<\/jats:italic>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-024-06350-1","type":"journal-article","created":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T17:02:12Z","timestamp":1720544532000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A new diagnostic technique for identifying Angiostrongylus spp. larvae in intermediate snail species by examining the buccal cavity"],"prefix":"10.1186","volume":"17","author":[{"given":"Yong-bo","family":"Zhao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Fang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shao-rong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-hua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shen-hua","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter S.","family":"Andrus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian-mei","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun-hai","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,7,9]]},"reference":[{"key":"6350_CR1","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1051\/parasite\/1935134312","volume":"13","author":"HT Chen","year":"1935","unstructured":"Chen HT. 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