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The gene encoding this transcription factor, <jats:italic>Pf<\/jats:italic>AP2-EXP2, is essential and thus likely subject to purifying selection that limits variants in the parasite population despite its genomic location.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>72 <jats:italic>Plasmodium falciparum<\/jats:italic> field samples and 801 clinical sequences from the Pf6 MalariaGEN dataset of Ghanaian origin, were integrated and analysed.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>A total of 14 single nucleotide variants of which 5 were missense variants, were identified after quality checks and filtering. Except for one, all identified variants were rare among the clinical samples obtained in this study (Minor allelic frequency\u2009&lt;\u20090.01). Further results revealed a considerably low dN\/dS value (0.208) suggesting the presence of purifying selection. Further, all the mutant amino acids were wildtype residues in <jats:italic>AP2-EXP2<\/jats:italic> orthologous proteins\u2014tentatively suggesting a genus-level conservation of amino acid residues. Computational analysis and predictions corroborated these findings.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Despite the recent extensive selective sweep within chromosome 6 of West African isolates, <jats:italic>Pf<\/jats:italic>AP2-EXP2 of Ghanaian origin exhibits low nucleotide diversity and very low dN\/dS consistent with purifying selection acting to maintain the function of an essential gene. The conservation of AP2-EXP2 is an important factor that makes it a potential drug target.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-024-06545-6","type":"journal-article","created":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T16:04:55Z","timestamp":1730822695000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Low nucleotide diversity of the Plasmodium falciparum AP2-EXP2 gene among clinical samples from Ghana"],"prefix":"10.1186","volume":"17","author":[{"given":"Elvis","family":"Quansah","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth Kofi","family":"Eduful","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enock Kofi","family":"Amoako","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas","family":"Amenga-Etego","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Faustina","family":"Halm-Lai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingli","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jilong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,11,5]]},"reference":[{"key":"6545_CR1","doi-asserted-by":"publisher","first-page":"618430","DOI":"10.3389\/fcimb.2020.618430","volume":"10","author":"S Briquet","year":"2021","unstructured":"Briquet S, Marinach C, Silvie O, Vaquero C. 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