{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T03:55:53Z","timestamp":1781927753134,"version":"3.54.5"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Inner Mongolia Medical University General Program","award":["YKD2024MS029"],"award-info":[{"award-number":["YKD2024MS029"]}]},{"name":"the National Key Research and Development Program of China","award":["2022YFC2601900"],"award-info":[{"award-number":["2022YFC2601900"]}]},{"DOI":"10.13039\/501100001809","name":"the National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82272327"],"award-info":[{"award-number":["82272327"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Bethune Project of Jilin University","award":["2022B01"],"award-info":[{"award-number":["2022B01"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Songling virus (SGLV) within the genus <jats:italic>Orthonairovirus<\/jats:italic>, family <jats:italic>Nairoviridae<\/jats:italic>, is an emerging tick-borne virus associated with human febrile illness. However, no rapid detection method for SGLV has been established.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>In this study, four primer sets targeting the nucleocapsid protein gene of SGLV were designed for use in the LAMP assay and evaluated to identify the optimal primer set. Recombinant plasmids were constructed and utilized for assessing the sensitivity of the assay. Tacheng tick virus 1 (TcTV-1)-, Beiji nairovirus (BJNV)-, Yezo virus (YEZV)-, severe fever with thrombocytopenia syndrome virus (SFTSV)-, and tick-borne encephalitis virus (TBEV)-positive tick samples were utilized to assess the specificity. Field-collected ticks were also evaluated as biological specimens to validate the assay.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>A SGLV-specific LAMP assay was established with a detection limit of 1\u2009\u00d7\u200910<jats:sup>\u20132<\/jats:sup>\u00a0copies\/\u03bcl and could be visually confirmed by a color change from purple to blue in SGLV-positive samples. No cross-reactivity was observed in the detection of TcTV-1, BJNV, YEZV, SFTSV, and TBEV using the LAMP assay. In addition to the detection of the same seven high-copy numbers of SGLV as the SYBR Green quantitative RT-PCR assay within a reduced timeframe, the developed LAMP method also effectively identified an additional sample with a low copy number in the field-collected tick samples.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>We successfully developed a sensitive, specific, and cost-effective visual method for the rapid detection of SGLV using the LAMP assay, which can be applied in pathogenesis and epidemiological surveillance studies of SGLV.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-024-06552-7","type":"journal-article","created":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T12:02:12Z","timestamp":1730462532000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Development of a LAMP assay for the rapid visual detection of the emerging tick-borne Songling virus"],"prefix":"10.1186","volume":"17","author":[{"given":"Zheng","family":"Gui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanning","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiqi","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiying","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunzhi","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingfeng","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lichao","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zedong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,11,1]]},"reference":[{"key":"6552_CR1","doi-asserted-by":"publisher","first-page":"19824","DOI":"10.1038\/s41598-023-46879-2","volume":"13","author":"K Ergunay","year":"2023","unstructured":"Ergunay K, Bourke BP, Reinbold-Wasson DD, Nikolich MP, Nelson SP, Caicedo-Quiroga L, et al. The expanding range of emerging tick-borne viruses in eastern Europe and the Black Sea region. Sci Rep. 2023;13:19824.","journal-title":"Sci Rep"},{"key":"6552_CR2","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1038\/s41579-022-00845-3","volume":"21","author":"H Zhou","year":"2023","unstructured":"Zhou H, Xu L, Shi W. The human-infection potential of emerging tick-borne viruses is a global public health concern. Nat Rev Microbiol. 2023;21:215\u20137.","journal-title":"Nat Rev Microbiol"},{"key":"6552_CR3","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/j.antiviral.2013.07.006","volume":"100","author":"DA Bente","year":"2013","unstructured":"Bente DA, Forrester NL, Watts DM, McAuley AJ, Whitehouse CA, Bray M. Crimean-Congo hemorrhagic fever: history, epidemiology, pathogenesis, clinical syndrome and genetic diversity. Antivir Res. 2013;100:159\u201389.","journal-title":"Antivir Res"},{"key":"6552_CR4","doi-asserted-by":"publisher","first-page":"1250","DOI":"10.3390\/v13071250","volume":"13","author":"J Hartlaub","year":"2021","unstructured":"Hartlaub J, Gutjahr B, Fast C, Mirazimi A, Keller M, Groschup MH. Diagnosis and pathogenesis of Nairobi sheep disease orthonairovirus infections in sheep and cattle. Viruses. 2021;13:1250.","journal-title":"Viruses"},{"key":"6552_CR5","doi-asserted-by":"publisher","first-page":"372","DOI":"10.3390\/v13030372","volume":"13","author":"J Hartlaub","year":"2021","unstructured":"Hartlaub J, von Arnim F, Fast C, Mirazimi A, Keller M, Groschup MH. Experimental challenge of sheep and cattle with Dugbe orthonairovirus, a neglected African arbovirus distantly related to CCHFV. Viruses. 2021;13:372.","journal-title":"Viruses"},{"key":"6552_CR6","doi-asserted-by":"publisher","first-page":"797","DOI":"10.3201\/eid2904.220885","volume":"29","author":"X Lv","year":"2023","unstructured":"Lv X, Liu Z, Li L, Xu W, Yuan Y, Liang X, et al. Yezo virus infection in tick-bitten patient and ticks, northeastern China. Emerg Infect Dis. 2023;29:797\u2013800.","journal-title":"Emerg Infect Dis"},{"key":"6552_CR7","doi-asserted-by":"publisher","first-page":"434","DOI":"10.1038\/s41591-020-01228-y","volume":"27","author":"J Ma","year":"2021","unstructured":"Ma J, Lv XL, Zhang X, Han SZ, Wang ZD, Li L, et al. Identification of a new orthonairovirus associated with human febrile illness in China. Nat Med. 2021;27:434\u20139.","journal-title":"Nat Med"},{"key":"6552_CR8","first-page":"59","volume":"56","author":"A Moming","year":"2024","unstructured":"Moming A, Bai Y, Chen S, Wu Q, Wang J, Jin J, et al. Epidemiological surveys revealed the risk of TAMV spill-over from ticks to hosts. Infect Dis (London, England). 2024;56:59\u201365.","journal-title":"Infect Dis (London, England)"},{"key":"6552_CR9","doi-asserted-by":"publisher","first-page":"2944","DOI":"10.3201\/eid2612.202411","volume":"26","author":"AJ Schuh","year":"2020","unstructured":"Schuh AJ, Amman BR, Patel K, Sealy TK, Swanepoel R, Towner JS. Human-pathogenic Kasokero virus in field-collected ticks. Emerg Infect Dis. 2020;26:2944\u201350.","journal-title":"Emerg Infect Dis"},{"key":"6552_CR10","doi-asserted-by":"publisher","first-page":"138","DOI":"10.7589\/JWD-D-21-00137","volume":"59","author":"N Ji","year":"2023","unstructured":"Ji N, Wang N, Liu G, Zhao S, Liu Z, Tan W, et al. Tacheng tick virus 1 and Songling virus infection in great gerbils (Rhombomys opimus) in northwestern China. J Wildlife Dis. 2023;59:138\u201342.","journal-title":"J Wildlife Dis"},{"key":"6552_CR11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0011017","volume":"16","author":"Z Liu","year":"2022","unstructured":"Liu Z, Li L, Xu W, Yuan Y, Liang X, Zhang L, et al. Extensive diversity of RNA viruses in ticks revealed by metagenomics in northeastern China. PLoS Neglect Trop Dis. 2022;16:e0011017.","journal-title":"PLoS Neglect Trop Dis"},{"key":"6552_CR12","doi-asserted-by":"publisher","first-page":"2116","DOI":"10.1056\/NEJMoa1805068","volume":"380","author":"ZD Wang","year":"2019","unstructured":"Wang ZD, Wang B, Wei F, Han SZ, Zhang L, Yang ZT, et al. A new segmented virus associated with human febrile illness in China. New Engl J Med. 2019;380:2116\u201325.","journal-title":"New Engl J Med"},{"key":"6552_CR13","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1016\/S1473-3099(15)70051-4","volume":"15","author":"H Li","year":"2015","unstructured":"Li H, Zheng YC, Ma L, Jia N, Jiang BG, Jiang RR, et al. Human infection with a novel tick-borne Anaplasma species in China: a surveillance study. Lancet Infect Dis. 2015;15:663\u201370.","journal-title":"Lancet Infect Dis"},{"key":"6552_CR14","doi-asserted-by":"publisher","first-page":"1176","DOI":"10.1056\/NEJMc1303625","volume":"369","author":"N Jia","year":"2013","unstructured":"Jia N, Jiang JF, Huo QB, Jiang BG, Cao WC. Rickettsia sibirica subspecies sibirica BJ-90 as a cause of human disease. New Engl J Med. 2013;369:1176\u20138.","journal-title":"New Engl J Med"},{"key":"6552_CR15","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2024.107038","volume":"143","author":"Y Ogata","year":"2024","unstructured":"Ogata Y, Sato T, Kato K, Kikuchi K, Mitsuhashi K, Watari K, et al. A case of tick-borne Yezo virus infection: concurrent detection in the patient and tick. Int J Infect Dis. 2024;143:107038.","journal-title":"Int J Infect Dis"},{"key":"6552_CR16","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2022.199006","volume":"323","author":"X Cai","year":"2023","unstructured":"Cai X, Cai X, Xu Y, Shao Y, Fu L, Men X, et al. Virome analysis of ticks and tick-borne viruses in Heilongjiang and Jilin Provinces. China Virus Res. 2023;323:199006.","journal-title":"China Virus Res"},{"key":"6552_CR17","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1016\/j.talanta.2018.08.019","volume":"190","author":"JG Kim","year":"2018","unstructured":"Kim JG, Baek SH, Kim S, Kim HI, Lee SW, Phan LMT, et al. Rapid discriminative detection of dengue viruses via loop mediated isothermal amplification. Talanta. 2018;190:391\u20136.","journal-title":"Talanta"},{"key":"6552_CR18","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2021.45669","volume":"5","author":"DM Heithoff","year":"2022","unstructured":"Heithoff DM, Barnes LT, Mahan SP, Fox GN, Arn KE, Ettinger SJ, et al. Assessment of a smartphone-based loop-mediated isothermal amplification assay for detection of SARS-CoV-2 and influenza viruses. JAMA Netw Open. 2022;5:e2145669.","journal-title":"JAMA Netw Open"},{"key":"6552_CR19","doi-asserted-by":"publisher","first-page":"19","DOI":"10.3390\/v12010019","volume":"12","author":"S Silva","year":"2019","unstructured":"Silva S, Pardee K, Pena L. Loop-mediated isothermal amplification (LAMP) for the diagnosis of Zika virus: a review. Viruses. 2019;12:19.","journal-title":"Viruses"},{"key":"6552_CR20","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1186\/1743-422X-10-68","volume":"10","author":"D Hayasaka","year":"2013","unstructured":"Hayasaka D, Aoki K, Morita K. Development of simple and rapid assay to detect viral RNA of tick-borne encephalitis virus by reverse transcription-loop-mediated isothermal amplification. Virol J. 2013;10:68.","journal-title":"Virol J"},{"key":"6552_CR21","doi-asserted-by":"publisher","first-page":"1187","DOI":"10.1186\/s12879-021-06877-z","volume":"21","author":"Y Zhang","year":"2021","unstructured":"Zhang Y, Jiang L, Yang Y, Xie S, Yuan W, Wang Y. A tick bite patient with fever and meningitis co-infected with Rickettsia raoultii and Tacheng tick virus 1: a case report. BMC Infect Dis. 2021;21:1187.","journal-title":"BMC Infect Dis"},{"key":"6552_CR22","doi-asserted-by":"publisher","first-page":"1200","DOI":"10.1080\/22221751.2021.1936197","volume":"10","author":"YC Wang","year":"2021","unstructured":"Wang YC, Wei Z, Lv X, Han S, Wang Z, Fan C, et al. A new nairo-like virus associated with human febrile illness in China. Emerg Microbes Infect. 2021;10:1200\u20138.","journal-title":"Emerg Microbes Infect"},{"key":"6552_CR23","doi-asserted-by":"publisher","first-page":"1122","DOI":"10.1080\/22221751.2019.1645573","volume":"8","author":"L Zhu","year":"2019","unstructured":"Zhu L, Yin F, Moming A, Zhang J, Wang B, Gao L, et al. First case of laboratory-confirmed severe fever with thrombocytopenia syndrome disease revealed the risk of SFTSV infection in Xinjiang, China. Emerg Microbes Infect. 2019;8:1122\u20135.","journal-title":"Emerg Microbes Infect"},{"key":"6552_CR24","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1016\/j.ttbdis.2017.04.009","volume":"8","author":"RX Sun","year":"2017","unstructured":"Sun RX, Lai SJ, Yang Y, Li XL, Liu K, Yao HW, et al. Mapping the distribution of tick-borne encephalitis in mainland China. Ticks Tick-Borne Dis. 2017;8:631\u20139.","journal-title":"Ticks Tick-Borne Dis"},{"key":"6552_CR25","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1007\/s12250-020-00228-z","volume":"36","author":"X Zhang","year":"2021","unstructured":"Zhang X, Wang N, Wang Z, Che L, Chen C, Zhao WZ, et al. First fatal infection and phylodynamic analysis of severe fever with thrombocytopenia syndrome virus in Jilin Province, northeastern China. Virol Sin. 2021;36:329\u201332.","journal-title":"Virol Sin"}],"container-title":["Parasites &amp; Vectors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-024-06552-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13071-024-06552-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-024-06552-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T12:04:22Z","timestamp":1730462662000},"score":1,"resource":{"primary":{"URL":"https:\/\/parasitesandvectors.biomedcentral.com\/articles\/10.1186\/s13071-024-06552-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,1]]},"references-count":25,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["6552"],"URL":"https:\/\/doi.org\/10.1186\/s13071-024-06552-7","relation":{},"ISSN":["1756-3305"],"issn-type":[{"value":"1756-3305","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,1]]},"assertion":[{"value":"7 August 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 October 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 November 2024","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"447"}}