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In particular, assemblages A and B are zoonotic, capable of infecting both humans and animals worldwide, resulting in significant economic losses and public health challenges in epidemic regions. Thus, the development of rapid, accurate and non-laboratory-based diagnostic methods for infected animals is crucial for the effective prevention and control of giardiasis. Recent advancements in clustered, regularly interspaced, short palindromic repeats (CRISPR) and CRISPR-associated (Cas) protein (Cas12a) systems allow promising avenues for nucleic acid detection, characterized by their high flexibility, sensitivity and specificity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Combined\n                      <jats:underline>re<\/jats:underline>\n                      combinase\n                      <jats:underline>po<\/jats:underline>\n                      lymerase amplification and C\n                      <jats:underline>R<\/jats:underline>\n                      ISPR\/Cas12a sys\n                      <jats:underline>t<\/jats:underline>\n                      ems were combined and used as end-point diagnostic methods (termed REPORT) to detect\n                      <jats:italic>G. duodenalis<\/jats:italic>\n                      assemblage A and B. The diagnostic results can be observed by fluorescence readouts with the naked eye under blue light or colorimetric signals using a lateral flow strip (LFS).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The limit of detection (LOD) of the REPORT\u2011based\n                      <jats:italic>G. duodenalis<\/jats:italic>\n                      assemblage A detection was 2.04 CFU\/ml and 10 trophozoites per gram (TPG), and the LOD of assemblage B was 1.1 CFU\/ml and 10 cysts per gram (CPG). The REPORT\u2011based\n                      <jats:italic>G. duodenalis<\/jats:italic>\n                      assemblage A and assemblage B detection methods have strong specificity and no cross-reactivity with other assemblages of\n                      <jats:italic>G. duodenalis<\/jats:italic>\n                      or common enteric parasitic protozoa and have excellent performance in clinical sample detection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study presents a novel strategy for the direct identification of\n                      <jats:italic>G. duodenalis<\/jats:italic>\n                      assemblages A and B, requiring neither highly trained personnel nor costly specialized equipment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Graphical Abstract<\/jats:title>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-024-06559-0","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T10:39:36Z","timestamp":1731407976000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["End-point diagnostics of Giardia duodenalis assemblages A and B by combining RPA with CRISPR\/Cas12a from human fecal samples"],"prefix":"10.1186","volume":"17","author":[{"given":"Yilin","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuchang","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinfeng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyang","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yayun","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longxian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,11,12]]},"reference":[{"key":"6559_CR1","doi-asserted-by":"publisher","first-page":"e00384","DOI":"10.1128\/mSphere.00384-16","volume":"2","author":"K Horlock-Roberts","year":"2017","unstructured":"Horlock-Roberts K, Reaume C, Dayer G, Ouellet C, Cook N, Yee J. 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