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The <jats:italic>L. infantum<\/jats:italic> RPA-LF was also tested using clinical samples (bone marrow and skin) from infected and uninfected dogs.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The detection limits (analytical sensitivity) of the assays were 0.04\u00a0pg\/\u03bcl and 0.04\u00a0ng\/\u03bcl for <jats:italic>L. braziliensis<\/jats:italic> and <jats:italic>L. infantum<\/jats:italic> kDNA, respectively. Using clinical samples, the <jats:italic>L. infantum<\/jats:italic> RPA-LF successfully detected the parasite kDNA in bone marrow (21\/30; 70.0%) and skin samples (23\/30, 76.6%) from naturally infected dogs. We found an almost perfect agreement (kappa\u2009=\u20090.807) between RPA-LF for <jats:italic>L. infantum<\/jats:italic> and our reference quantitative real-time polymerase chain reaction (qPCR), considering clinical samples with a quantification cycle (<jats:italic>C<\/jats:italic><jats:sub>q<\/jats:sub>)\u2009&lt;\u200930, whereas the agreement with samples with a <jats:italic>C<\/jats:italic><jats:sub>q<\/jats:sub>\u2009&gt;\u200930 (lower parasite loads) was moderate (kappa\u2009=\u20090.440).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The RPA-LF assays developed here may be promising diagnostic tools for point-of-care diagnosis of <jats:italic>L. infantum<\/jats:italic> and <jats:italic>L. braziliensis<\/jats:italic> infection in dogs, particularly in remote rural areas lacking laboratory infrastructure.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Graphical Abstract<\/jats:title>\n                \n              <\/jats:sec>","DOI":"10.1186\/s13071-024-06633-7","type":"journal-article","created":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T07:42:53Z","timestamp":1736235773000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Rapid isothermal molecular tests to discriminate between Leishmania braziliensis and Leishmania infantum infections in dogs"],"prefix":"10.1186","volume":"18","author":[{"given":"Rafaela Lira Nogueira","family":"de Luna","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamila Gaud\u00eancio da Silva","family":"Sales","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Lisboa Nunes","family":"Bonif\u00e1cio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana Aguiar","family":"Figueredo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas R.","family":"Shelite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio dos Santos","family":"Nogueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Domenico","family":"Otranto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filipe","family":"Dantas-Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,7]]},"reference":[{"key":"6633_CR1","doi-asserted-by":"publisher","first-page":"531","DOI":"10.1016\/j.pt.2012.08.007","volume":"28","author":"F Dantas-Torres","year":"2012","unstructured":"Dantas-Torres F, Solano-Gallego L, Baneth G, Ribeiro VM, de Paiva-Cavalcanti M, Otranto D. 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The procedures of the current study were also assessed and authorized by the Ethics Committee on Animal Use of Aggeu Magalh\u00e3es Institute (CEUA n\u00ba 153\/19).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"Filipe Dantas-Torres is the Editor-in-Chief of <i>Parasites & Vectors<\/i>.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"2"}}