{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T11:02:58Z","timestamp":1769943778727,"version":"3.49.0"},"reference-count":39,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,1,25]],"date-time":"2025-01-25T00:00:00Z","timestamp":1737763200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,1,25]],"date-time":"2025-01-25T00:00:00Z","timestamp":1737763200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP210101500"],"award-info":[{"award-number":["DP210101500"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP210101500"],"award-info":[{"award-number":["DP210101500"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP210101500"],"award-info":[{"award-number":["DP210101500"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>\n              <jats:italic>Nippostrongylus brasiliensis<\/jats:italic>\u2014a nematode of rodents\u2014is commonly used as a model to study the immunobiology of parasitic nematodes. It is a member of the Strongylida\u2014a large order of socioeconomically important parasitic nematodes of animals. Lipids are known to play essential roles in nematode biology, influencing cellular membranes, energy storage and\/or signalling.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>The present investigation provides a comprehensive, untargeted lipidomic analysis of four developmental stages\/sexes (i.e.\u00a0egg, L3, adult female and adult male stages) of <jats:italic>N. brasiliensis<\/jats:italic> utilising liquid chromatography coupled to mass spectrometry.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>We identified 464 lipid species representing 18 lipid classes and revealed distinct stage-specific changes in lipid composition throughout nematode development. Triacylglycerols (TGs) dominated the lipid profile in the egg stage, suggesting a key role for them in energy storage at this early developmental stage. As <jats:italic>N. brasiliensis<\/jats:italic> develops, there was a conspicuous transition toward membrane-associated lipids, including glycerophospholipids (e.g.\u00a0PE and PC) and ether-linked lipids, particularly in adult stages, indicating a shift toward host adaptation and membrane stabilisation.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>We provide a comprehensive insight into the lipid composition and abundance of key free-living and parasitic stages of <jats:italic>N. brasiliensis<\/jats:italic>. This study provides lipidomic resources to underpin the detailed exploration of lipid biology in this model parasitic nematode.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Graphical Abstract<\/jats:title>\n          <\/jats:sec>","DOI":"10.1186\/s13071-024-06654-2","type":"journal-article","created":{"date-parts":[[2025,1,25]],"date-time":"2025-01-25T11:58:02Z","timestamp":1737806282000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The developmental lipidome of Nippostrongylus brasiliensis"],"prefix":"10.1186","volume":"18","author":[{"given":"Tao","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael G.","family":"Leeming","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas A.","family":"Williamson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiffany","family":"Bouchery","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rory","family":"Doolan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graham","family":"Le Gros","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gavin E.","family":"Reid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola L.","family":"Harris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robin B.","family":"Gasser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,1,25]]},"reference":[{"key":"6654_CR1","doi-asserted-by":"publisher","first-page":"16088","DOI":"10.1038\/nrdp.2016.88","volume":"2","author":"A Loukas","year":"2016","unstructured":"Loukas A, Hotez PJ, Diemert D, Yazdanbakhsh M, McCarthy JS, Correa-Oliveira R, et al. Hookworm infection. Nat Rev Dis Primers. 2016;2:16088. https:\/\/doi.org\/10.1038\/nrdp.2016.88.","journal-title":"Nat Rev Dis Primers"},{"key":"6654_CR2","unstructured":"Gasser RB, von Samson-Himmelstjerna G. Haemonchus contortus and Haemonchosis \u2013 Past, Present and Future Trends. Adv Parasitol. 2016. 93."},{"key":"6654_CR3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004922","volume":"10","author":"SM Bartsch","year":"2016","unstructured":"Bartsch SM, Hotez PJ, Asti L, Zapf KM, Bottazzi ME, Diemert DJ, et al. The global economic and health burden of human hookworm infection. PLoS Negl Trop Dis. 2016;10:e0004922. https:\/\/doi.org\/10.1371\/journal.pntd.0004922.","journal-title":"PLoS Negl Trop Dis"},{"key":"6654_CR4","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/bs.apar.2021.12.002","volume":"115","author":"J Charlier","year":"2022","unstructured":"Charlier J, Bartley DJ, Sotiraki S, Martinez-Valladares M, Claerebout E, von Samson-Himmelstjerna G, et al. Anthelmintic resistance in ruminants: challenges and solutions. Adv Parasitol. 2022;115:171\u2013227. https:\/\/doi.org\/10.1016\/bs.apar.2021.12.002.","journal-title":"Adv Parasitol"},{"key":"6654_CR5","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1016\/bs.apar.2016.02.012","volume":"93","author":"AC Kotze","year":"2016","unstructured":"Kotze AC, Prichard RK. Anthelmintic resistance in Haemonchus contortus: History, mechanisms and diagnosis. Adv Parasitol. 2016;93:397\u2013428. https:\/\/doi.org\/10.1016\/bs.apar.2016.02.012.","journal-title":"Adv Parasitol"},{"key":"6654_CR6","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.ijpddr.2022.10.005","volume":"20","author":"MK Nielsen","year":"2022","unstructured":"Nielsen MK. Anthelmintic resistance in equine nematodes: current status and emerging trends. Int J Parasitol Drugs Drug Resist. 2022;20:76\u201388. https:\/\/doi.org\/10.1016\/j.ijpddr.2022.10.005.","journal-title":"Int J Parasitol Drugs Drug Resist"},{"key":"6654_CR7","doi-asserted-by":"publisher","first-page":"616","DOI":"10.3390\/ph14070616","volume":"14","author":"AC Taki","year":"2021","unstructured":"Taki AC, Byrne JJ, Wang T, Sleebs BE, Nguyen N, Hall RS, et al. High-Throughput phenotypic assay to screen for anthelmintic activity on Haemonchus contortus. Pharmaceuticals. 2021;14:616. https:\/\/doi.org\/10.3390\/ph14070616.","journal-title":"Pharmaceuticals"},{"key":"6654_CR8","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1586\/14760584.2014.872035","volume":"13","author":"B Zhan","year":"2014","unstructured":"Zhan B, Beaumier CM, Briggs N, Jones KM, Keegan BP, Bottazzi ME, et al. Advancing a multivalent \u201cPan-anthelmintic\u201d vaccine against soil-transmitted nematode infections. Expert Rev Vaccines. 2014;13:321\u201331. https:\/\/doi.org\/10.1586\/14760584.2014.872035.","journal-title":"Expert Rev Vaccines"},{"key":"6654_CR9","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1016\/bs.apar.2016.02.011","volume":"93","author":"AJ Nisbet","year":"2016","unstructured":"Nisbet AJ, Meeusen EN, Gonzalez JF, Piedrafita DM. Immunity to Haemonchus contortus and vaccine development. Adv Parasitol. 2016;93:353\u201396. https:\/\/doi.org\/10.1016\/bs.apar.2016.02.011.","journal-title":"Adv Parasitol"},{"key":"6654_CR10","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/0014-4894(66)90056-7","volume":"19","author":"A Greichus","year":"1966","unstructured":"Greichus A, Greichus YA. Chemical composition and volatile fatty acid production of male Ascaris lumbricoides before and after starvation. Exp Parasitol. 1966;19:85\u201390. https:\/\/doi.org\/10.1016\/0014-4894(66)90056-7.","journal-title":"Exp Parasitol"},{"key":"6654_CR11","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/s0166-6851(96)02615-1","volume":"78","author":"VP Smith","year":"1996","unstructured":"Smith VP, Selkirk ME, Gounaris K. Identification and composition of lipid classes in surface and somatic preparations of adult Brugia malayi. Mol Biochem Parasitol. 1996;78:105\u201316. https:\/\/doi.org\/10.1016\/s0166-6851(96)02615-1.","journal-title":"Mol Biochem Parasitol"},{"key":"6654_CR12","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.molbiopara.2017.06.008","volume":"216","author":"AC Becker","year":"2017","unstructured":"Becker AC, Willenberg I, Springer A, Schebb NH, Steinberg P, Strube C. Fatty acid composition of free-living and parasitic stages of the bovine lungworm Dictyocaulus viviparus. Mol Biochem Parasitol. 2017;216:39\u201344. https:\/\/doi.org\/10.1016\/j.molbiopara.2017.06.008.","journal-title":"Mol Biochem Parasitol"},{"key":"6654_CR13","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1017\/s0022149x00012347","volume":"64","author":"T Minematsu","year":"1990","unstructured":"Minematsu T, Yamazaki S, Uji Y, Okabe H, Korenaga M, Tada I. Analysis of polyunsaturated fatty acid composition of Strongyloides ratti in relation to development. J Helminthol. 1990;64:303\u20139. https:\/\/doi.org\/10.1017\/s0022149x00012347.","journal-title":"J Helminthol"},{"key":"6654_CR14","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1021\/acs.analchem.7b04836","volume":"90","author":"YH Rustam","year":"2018","unstructured":"Rustam YH, Reid GE. Analytical challenges and recent advances in mass spectrometry based lipidomics. Anal Chem. 2018;90:374\u201397. https:\/\/doi.org\/10.1021\/acs.analchem.7b04836.","journal-title":"Anal Chem"},{"key":"6654_CR15","doi-asserted-by":"publisher","DOI":"10.1016\/j.crpvbd.2021.100018","author":"T Wang","year":"2021","unstructured":"Wang T, Nie S, Reid GE, Gasser RB. Helminth lipidomics\u2013technical aspects and future prospects. Curr Res Parasitol Vect Dis. 2021. https:\/\/doi.org\/10.1016\/j.crpvbd.2021.100018.","journal-title":"Curr Res Parasitol Vect Dis"},{"key":"6654_CR16","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1016\/j.ijpara.2017.06.001","volume":"47","author":"V Wewer","year":"2017","unstructured":"Wewer V, Makepeace BL, Tanya VN, Peisker H, Pfarr K, Hoerauf A, et al. Lipid profiling of the filarial nematodes Onchocerca volvulus, Onchocerca ochengi and Litomosoides sigmodontis reveals the accumulation of nematode-specific ether phospholipids in the host. Int J Parasitol. 2017;47:903\u201312. https:\/\/doi.org\/10.1016\/j.ijpara.2017.06.001.","journal-title":"Int J Parasitol"},{"key":"6654_CR17","doi-asserted-by":"publisher","first-page":"887","DOI":"10.1016\/j.ijpara.2018.06.002","volume":"48","author":"T Wang","year":"2018","unstructured":"Wang T, Nie S, Ma G, Korhonen PK, Koehler AV, Ang CS, et al. The developmental lipidome of Haemonchus contortus. Int J Parasitol. 2018;48:887\u201395. https:\/\/doi.org\/10.1016\/j.ijpara.2018.06.002.","journal-title":"Int J Parasitol"},{"key":"6654_CR18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008848","author":"T Wang","year":"2020","unstructured":"Wang T, Nie S, Ma G, Vlaminck J, Geldhof P, Williamson NA, et al. Quantitative lipidomic analysis of Ascaris suum. PLoS Negl Trop Dis. 2020. https:\/\/doi.org\/10.1371\/journal.pntd.0008848.","journal-title":"PLoS Negl Trop Dis"},{"key":"6654_CR19","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-018-3265-y","author":"G Ma","year":"2019","unstructured":"Ma G, Wang T, Korhonen PK, Nie S, Reid GE, Stroehlein AJ, et al. Comparative bioinformatic analysis suggests that specific dauer-like signalling pathway components regulate Toxocara canis development and migration in the mammalian host. Parasit Vectors. 2019. https:\/\/doi.org\/10.1186\/s13071-018-3265-y.","journal-title":"Parasit Vectors"},{"key":"6654_CR20","doi-asserted-by":"publisher","first-page":"30741","DOI":"10.3402\/jev.v5.30741","volume":"5","author":"F Simbari","year":"2016","unstructured":"Simbari F, McCaskill J, Coakley G, Millar M, Maizels RM, Fabrias G, et al. Plasmalogen enrichment in exosomes secreted by a nematode parasite versus those derived from its mouse host: implications for exosome stability and biology. J Extracell Vesicles. 2016;5:30741. https:\/\/doi.org\/10.3402\/jev.v5.30741.","journal-title":"J Extracell Vesicles"},{"key":"6654_CR21","doi-asserted-by":"publisher","DOI":"10.1002\/0471142735.im1912s55","author":"M Camberis","year":"2003","unstructured":"Camberis M, Le Gros G, Urban J Jr. Animal model of Nippostrongylus brasiliensis and Heligmosomoides polygyrus. Curr Protoc Immunol. 2003. https:\/\/doi.org\/10.1002\/0471142735.im1912s55.","journal-title":"Curr Protoc Immunol"},{"key":"6654_CR22","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12717","volume":"42","author":"G Coakley","year":"2020","unstructured":"Coakley G, Harris NL. Interactions between macrophages and helminths. Parasite Immunol. 2020;42:e12717. https:\/\/doi.org\/10.1111\/pim.12717.","journal-title":"Parasite Immunol"},{"key":"6654_CR23","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1016\/j.pt.2020.07.002","volume":"36","author":"G Coakley","year":"2020","unstructured":"Coakley G, Harris NL. The intestinal epithelium at the forefront of host-helminth interactions. Trends Parasitol. 2020;36:761\u201372. https:\/\/doi.org\/10.1016\/j.pt.2020.07.002.","journal-title":"Trends Parasitol"},{"key":"6654_CR24","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12429","author":"K Filbey","year":"2018","unstructured":"Filbey K, Bouchery T, Le Gros G. The role of ILC2 in hookworm infection. Parasite Immunol. 2018. https:\/\/doi.org\/10.1111\/pim.12429.","journal-title":"Parasite Immunol"},{"key":"6654_CR25","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1111\/imm.12601","volume":"148","author":"MG Nair","year":"2016","unstructured":"Nair MG, Herbert DR. Immune polarization by hookworms: taking cues from T helper type 2, type 2 innate lymphoid cells and alternatively activated macrophages. Immunology. 2016;148:115\u201324. https:\/\/doi.org\/10.1111\/imm.12601.","journal-title":"Immunology"},{"key":"6654_CR26","first-page":"131","volume":"57","author":"G Baermann","year":"1917","unstructured":"Baermann G. Eine einfache methode zur auffindung von anklostomum (nematoden) larven in erdproben. Tijdschr Diergeneeskd. 1917;57:131\u20137.","journal-title":"Tijdschr Diergeneeskd"},{"key":"6654_CR27","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12089","volume":"10","author":"H Su","year":"2021","unstructured":"Su H, Rustam YH, Masters CL, Makalic E, McLean CA, Hill AF, et al. Characterization of brain-derived extracellular vesicle lipids in Alzheimer\u2019s disease. J Extracell Vesicles. 2021;10:e12089. https:\/\/doi.org\/10.1002\/jev2.12089.","journal-title":"J Extracell Vesicles"},{"key":"6654_CR28","doi-asserted-by":"publisher","first-page":"1159","DOI":"10.1038\/s41587-020-0531-2","volume":"38","author":"H Tsugawa","year":"2020","unstructured":"Tsugawa H, Ikeda K, Takahashi M, Satoh A, Mori Y, Uchino H, et al. A lipidome atlas in MS-DIAL 4. Nat Biotechnol. 2020;38:1159\u201363. https:\/\/doi.org\/10.1038\/s41587-020-0531-2.","journal-title":"Nat Biotechnol"},{"key":"6654_CR29","doi-asserted-by":"publisher","first-page":"1539","DOI":"10.1194\/jlr.S120001025","volume":"61","author":"G Liebisch","year":"2020","unstructured":"Liebisch G, Fahy E, Aoki J, Dennis EA, Durand T, Ejsing CS, et al. Update on LIPID MAPS classification, nomenclature, and shorthand notation for MS-derived lipid structures. J Lipid Res. 2020;61:1539\u201355. https:\/\/doi.org\/10.1194\/jlr.S120001025.","journal-title":"J Lipid Res"},{"key":"6654_CR30","doi-asserted-by":"publisher","first-page":"21","DOI":"10.3390\/metabo1010021","volume":"1","author":"K Yang","year":"2011","unstructured":"Yang K, Han X. Accurate quantification of lipid species by electrospray ionization mass spectrometry\u2014meet a key challenge in lipidomics. Metabolites. 2011;1:21\u201340. https:\/\/doi.org\/10.3390\/metabo1010021.","journal-title":"Metabolites"},{"key":"6654_CR31","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1038\/nmeth.3901","volume":"13","author":"S Tyanova","year":"2016","unstructured":"Tyanova S, Temu T, Sinitcyn P, Carlson A, Hein MY, Geiger T, et al. The Perseus computational platform for comprehensive analysis of (prote)omics data. Nat Methods. 2016;13:731\u201340. https:\/\/doi.org\/10.1038\/nmeth.3901.","journal-title":"Nat Methods"},{"key":"6654_CR32","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-017-1708-7","author":"A Khan","year":"2017","unstructured":"Khan A, Mathelier A. Intervene: a tool for intersection and visualization of multiple gene or genomic region sets. BMC Bioinform. 2017. https:\/\/doi.org\/10.1186\/s12859-017-1708-7.","journal-title":"BMC Bioinform"},{"key":"6654_CR33","doi-asserted-by":"publisher","DOI":"10.3390\/metabo10110446","author":"K Yeshi","year":"2020","unstructured":"Yeshi K, Creek DJ, Anderson D, Ritmejeryte E, Becker L, Loukas A, et al. Metabolomes and lipidomes of the infective stages of the gastrointestinal nematodes Nippostrongylus brasiliensis and Trichuris muris. Metabolites. 2020. https:\/\/doi.org\/10.3390\/metabo10110446.","journal-title":"Metabolites"},{"key":"6654_CR34","doi-asserted-by":"publisher","first-page":"338","DOI":"10.1186\/s13071-020-04208-w","volume":"13","author":"T Wang","year":"2020","unstructured":"Wang T, Ma G, Nie S, Williamson NA, Reid GE, Gasser RB. Lipid composition and abundance in the reproductive and alimentary tracts of female Haemonchus contortus. Parasit Vectors. 2020;13:338. https:\/\/doi.org\/10.1186\/s13071-020-04208-w.","journal-title":"Parasit Vectors"},{"key":"6654_CR35","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1016\/j.aca.2017.11.058","volume":"1037","author":"M Giera","year":"2018","unstructured":"Giera M, Kaisar MMM, Derks RJE, Steenvoorden E, Kruize YCM, Hokke CH, et al. The Schistosoma mansoni lipidome: leads for immunomodulation. Anal Chim Acta. 2018;1037:107\u201318. https:\/\/doi.org\/10.1016\/j.aca.2017.11.058.","journal-title":"Anal Chim Acta"},{"key":"6654_CR36","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1016\/j.chemphyslip.2011.06.008","volume":"164","author":"S Wallner","year":"2011","unstructured":"Wallner S, Schmitz G. Plasmalogens the neglected regulatory and scavenging lipid species. Chem Phys Lipids. 2011;164:573\u201389. https:\/\/doi.org\/10.1016\/j.chemphyslip.2011.06.008.","journal-title":"Chem Phys Lipids"},{"key":"6654_CR37","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1194\/jlr.M064808","volume":"57","author":"X Shi","year":"2016","unstructured":"Shi X, Tarazona P, Brock TJ, Browse J, Feussner I, Watts JL. A Caenorhabditis elegans model for ether lipid biosynthesis and function. J Lipid Res. 2016;57:265\u201375. https:\/\/doi.org\/10.1194\/jlr.M064808.","journal-title":"J Lipid Res"},{"key":"6654_CR38","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1016\/s0891-5849(98)00221-4","volume":"26","author":"PJ Sindelar","year":"1999","unstructured":"Sindelar PJ, Guan Z, Dallner G, Ernster L. The protective role of plasmalogens in iron-induced lipid peroxidation. Free Radic Biol Med. 1999;26:318\u201324. https:\/\/doi.org\/10.1016\/s0891-5849(98)00221-4.","journal-title":"Free Radic Biol Med"},{"key":"6654_CR39","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/j.ijpara.2015.03.011","volume":"45","author":"K Retra","year":"2015","unstructured":"Retra K, deWalick S, Schmitz M, Yazdanbakhsh M, Tielens AG, Brouwers JF, et al. The tegumental surface membranes of Schistosoma mansoni are enriched in parasite-specific phospholipid species. Int J Parasitol. 2015;45:629\u201336. https:\/\/doi.org\/10.1016\/j.ijpara.2015.03.011.","journal-title":"Int J Parasitol"}],"container-title":["Parasites &amp; Vectors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-024-06654-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13071-024-06654-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-024-06654-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,25]],"date-time":"2025-01-25T11:58:05Z","timestamp":1737806285000},"score":1,"resource":{"primary":{"URL":"https:\/\/parasitesandvectors.biomedcentral.com\/articles\/10.1186\/s13071-024-06654-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,25]]},"references-count":39,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["6654"],"URL":"https:\/\/doi.org\/10.1186\/s13071-024-06654-2","relation":{},"ISSN":["1756-3305"],"issn-type":[{"value":"1756-3305","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,25]]},"assertion":[{"value":"18 November 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 December 2024","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 January 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The animal ethics approval for sample collection was obtained from Monash University (permit no. E\/1843\/2018\/M).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"27"}}