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Despite the availability of preventatives, compliance can be lacking. Increasing pet owner options and combining endo- and ectoparasite treatments might improve this compliance. In four separate studies, we investigated the efficacy of Simparica Trio\u00ae (Zoetis Inc., Parsippany, NJ, USA) containing minimum dosages of 1.2\u00a0mg\/kg sarolaner, 24\u00a0\u00b5g\/kg moxidectin and 5\u00a0mg\/kg pyrantel against fourth- and fifth-stage larvae (L<jats:sub>4<\/jats:sub> and L<jats:sub>5<\/jats:sub>, respectively) and adults of <jats:italic>A. braziliense<\/jats:italic> and adults of <jats:italic>A<\/jats:italic>. <jats:italic>ceylanicum<\/jats:italic> in dogs.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Four negatively controlled, randomised and blinded laboratory studies were conducted against induced infections of <jats:italic>A. braziliense<\/jats:italic> and <jats:italic>A. ceylanicum<\/jats:italic>, with the interval between infection and treatment selected to evaluate efficacy against each targeted stage. Each treated dog received a single oral dose of Simparica Trio\u00ae at the recommended label dose. Necropsy was conducted for worm recovery on day 7 or 8 post-treatment.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>No treatment-related adverse events were recorded in any of the studies. No worms were recovered from any of the Simparica Trio\u00ae-treated dogs in all four studies, thus resulting in 100% efficacy (<jats:italic>P<\/jats:italic>\u2009\u2264\u20090.0005) of Simparica Trio\u00ae against all stages of <jats:italic>A. braziliense<\/jats:italic> and the adult stage of <jats:italic>A. ceylanicum<\/jats:italic> in dogs.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Simparica Trio\u00ae containing sarolaner, moxidectin and pyrantel was highly effective against induced infections of L<jats:sub>4<\/jats:sub>, L<jats:sub>5<\/jats:sub> and adult stages of <jats:italic>A. braziliense<\/jats:italic> and the adult stage of <jats:italic>A. ceylanicum<\/jats:italic> in dogs.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Graphical Abstract<\/jats:title>\n          <\/jats:sec>","DOI":"10.1186\/s13071-025-06758-3","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T03:42:10Z","timestamp":1745811730000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Efficacy of Simparica Trio\u00ae against induced infections of Ancylostoma braziliense and Ancylostoma ceylanicum in dogs"],"prefix":"10.1186","volume":"18","author":[{"given":"Raj","family":"Packianathan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Hodge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalie","family":"Bruellke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Pearce","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frans","family":"Selepe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piyanan","family":"Taweethavonsawat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Geurden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,4,28]]},"reference":[{"key":"6758_CR1","doi-asserted-by":"publisher","first-page":"e72","DOI":"10.1016\/S2666-5247(21)00181-6","volume":"3","author":"ACA Clements","year":"2022","unstructured":"Clements ACA, Addis AK. 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