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The lethal concentration 50 (concentration causing 50% mortality [LC50]) values were calculated to determine the resistance ratios (RRs) of the populations. The lethal concentration 99 (concentration causing 99% mortality [LC99]) values were calculated to determine the discriminating doses (DDs\u2009=\u20092\u2009\u00d7\u2009LC99). The DDs were applied in tests with the POA (susceptible) and GYN (resistant) strains, as well as in tests with the population that presented the lowest LC50 value (population 14) and with the two populations that presented the highest LC50 value (populations 10 and 16). Finally, we performed field trials with the population that presented the lowest and two highest LC50 values.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>In the LIT with fluralaner, the LC50 values ranged from 0.144 to 0.481\u00a0\u00b5g\/mL for the 18<jats:italic> R. microplus<\/jats:italic> populations. The mortality rate was 100% in the tests of the DDs in the five populations tested. In the field trials, the efficacy of fluralaner was similar for the three populations of <jats:italic>R. microplus<\/jats:italic> tested (populations 14, 10 and 16), with therapeutic efficacy (until day 21) of 100% and persistent efficacy (between days 28 and 42)\u2009&gt;\u200995%.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>We observed natural variability in the susceptibility of larvae from the different populations of <jats:italic>R. microplus<\/jats:italic> that had never been treated with this compound. Despite the observed variability in the in vitro results (LC50), a comparable efficacy of &gt; 90% lasting until day\u200942 was observed in the field trials. Also, based on the results of the laboratory testing (LC50 and DD) and field trials, we can conclude that there was no resistance to fluralaner in the 18 studied tick populations.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Graphical Abstract<\/jats:title>\n          <\/jats:sec>","DOI":"10.1186\/s13071-025-06775-2","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T16:59:49Z","timestamp":1745859589000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Acaricidal efficacy of fluralaner against Rhipicephalus microplus ticks under laboratory and field conditions in Brazil"],"prefix":"10.1186","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9792-5744","authenticated-orcid":false,"given":"Daniel de Castro","family":"Rodrigues","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9731-6954","authenticated-orcid":false,"given":"Gabriel Webert","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1449-2582","authenticated-orcid":false,"given":"Francisca Leticia","family":"Vale","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2851-3102","authenticated-orcid":false,"given":"Ana L\u00facia Coutinho","family":"Teixeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2992-9636","authenticated-orcid":false,"given":"Isabela Santos","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8178-7997","authenticated-orcid":false,"given":"Haile Dean Figueiredo","family":"Chagas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1490-5801","authenticated-orcid":false,"given":"Tom","family":"Strydom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3315-894X","authenticated-orcid":false,"given":"Siddartha","family":"Torres","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7694-2817","authenticated-orcid":false,"given":"Rafael Paranhos","family":"de Mendon\u00e7a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8888-6269","authenticated-orcid":false,"given":"Fernando","family":"de Almeida Borges","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1475-049X","authenticated-orcid":false,"given":"L\u00edvio","family":"Costa Junior","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0813-3054","authenticated-orcid":false,"given":"Lorena Lopes","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8009-8436","authenticated-orcid":false,"given":"Welber Daniel Zanetti","family":"Lopes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5253-5451","authenticated-orcid":false,"given":"Caio","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,4,28]]},"reference":[{"key":"6775_CR1","unstructured":"Martins JRS, Furlong J, Leite RC. 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