{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T04:27:31Z","timestamp":1772166451876,"version":"3.50.1"},"reference-count":131,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T00:00:00Z","timestamp":1752019200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T00:00:00Z","timestamp":1752019200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"National Science Foundation","award":["DEB-2030345"],"award-info":[{"award-number":["DEB-2030345"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The blowfly\n                      <jats:italic>Lucilia cuprina<\/jats:italic>\n                      is a destructive parasite of sheep that causes flystrike or myiasis. Larvae consume the animal\u2019s living flesh, producing large wounds that can lead to death. The main aim of this study was to identify genes that may play important roles in the behavior and physiology of\n                      <jats:italic>L. cuprina<\/jats:italic>\n                      larvae.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      An RNA-Seq analysis of RNA from whole larvae at different developmental stages and third-instar head and gut tissues was used to identify sensory receptors and other genes relevant to the physiology of\n                      <jats:italic>L. cuprina<\/jats:italic>\n                      larvae. In addition, CRISPR\/Cas9 gene editing was used to obtain a loss-of-function mutation for the\n                      <jats:italic>L. cuprina<\/jats:italic>\n                      odorant coreceptor gene (\n                      <jats:italic>LcupOrco<\/jats:italic>\n                      ). The response of mutant larvae and adult females to fresh and rotten meat at different temperatures was evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The RNA-Seq analysis suggested that odorant (OR), gustatory, ionotropic, and\n                      <jats:italic>Pickpocket<\/jats:italic>\n                      receptors may not play a central role in the\n                      <jats:italic>L. cuprina<\/jats:italic>\n                      larval sensory signaling and digestive systems. Rather, ATP-binding cassettes (ABCs) were highly enriched in head and gut RNA, and odorant-binding proteins (OBPs) only in the head. To confirm that ORs are not essential for larval detection of rotten beef, diet-choice assays were performed including larvae and adults homozygous for a null mutation in\n                      <jats:italic>LcupOrco<\/jats:italic>\n                      . While the attraction of adult females to rotten beef was disrupted,\n                      <jats:italic>LcupOrco<\/jats:italic>\n                      mutant larvae showed no change in diet preference.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The expression pattern of the ABC and OBP gene families suggests a central role in the sensory system of the\n                      <jats:italic>L. cuprina<\/jats:italic>\n                      larva for these receptors. Behavioral assays showed that ORs are essential for the adult female response to rotten beef, but not for larval behavior. These findings are consistent with high levels of expression of\n                      <jats:italic>LcupOrco<\/jats:italic>\n                      in the adult female antenna but very low expression in larvae.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Graphical abstract<\/jats:title>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-025-06804-0","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T08:41:24Z","timestamp":1752136884000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genetic and behavioral analyses suggest that larval and adult stages of Lucilia cuprina employ different sensory systems to detect rotten beef"],"prefix":"10.1186","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5773-4684","authenticated-orcid":false,"given":"Juan P.","family":"Wulff","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel K.","family":"Laminack","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6536-4735","authenticated-orcid":false,"given":"Maxwell J.","family":"Scott","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,7,9]]},"reference":[{"key":"6804_CR1","doi-asserted-by":"publisher","DOI":"10.1201\/b18819","volume-title":"Carrion ecology, evolution, and their applications","author":"ME Benbow","year":"2015","unstructured":"Benbow ME, Tomberlin JK, Tarone AM. Carrion ecology, evolution, and their applications. In: Benbow ME, Tomberlin JK, Tarone AM, editors. Carrion ecology, evolution, and their applications. Boca Raton: CRC Press; 2015."},{"key":"6804_CR2","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.vetpar.2011.05.030","volume":"180","author":"R Wall","year":"2011","unstructured":"Wall R, Rose H, Ellse L, Morgan E. Livestock ectoparasites: integrated management in a changing climate. Vet Parasitol. 2011;180:82\u20139.","journal-title":"Vet Parasitol"},{"key":"6804_CR3","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1111\/j.1461-0248.2010.01566.x","volume":"14","author":"WM Getz","year":"2011","unstructured":"Getz WM. Biomass transformation webs provide a unified approach to consumer-resource modelling. Ecol Lett. 2011;14:113\u201324.","journal-title":"Ecol Lett"},{"key":"6804_CR4","doi-asserted-by":"publisher","first-page":"930","DOI":"10.3390\/insects3040930","volume":"3","author":"T Mastrangelo","year":"2012","unstructured":"Mastrangelo T, Welch J. An overview of the components of AW-IPM campaigns against the new world screwworm. Insects. 2012;3:930\u201355.","journal-title":"Insects"},{"key":"6804_CR5","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1016\/j.vetpar.2006.01.006","volume":"137","author":"ACG Heath","year":"2006","unstructured":"Heath ACG, Bishop DM. Flystrike in New Zealand: an overview based on a 16-year study, following the introduction and dispersal of the Australian sheep blowfly, Lucilia cuprina Wiedemann (Diptera: Calliphoridae). Vet Parasitol. 2006;137:333\u201344.","journal-title":"Vet Parasitol"},{"key":"6804_CR6","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1111\/j.1365-2915.2007.00675.x","volume":"21","author":"KG Wardhaugh","year":"2007","unstructured":"Wardhaugh KG, Morton R, Bedo D, Horton BJ, Mahon RJ. Estimating the incidence of fly myiases in Australian sheep flocks: development of a weather-driven regression model. Med Vet Entomol. 2007;21:153\u201367. https:\/\/doi.org\/10.1111\/j.1365-2915.2007.00675.x.","journal-title":"Med Vet Entomol"},{"key":"6804_CR7","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1080\/00480169.2014.960500","volume":"63","author":"A Heath","year":"2015","unstructured":"Heath A, Levot G. Parasiticide resistance in flies, lice and ticks in New Zealand and Australia: mechanisms, prevalence and prevention. N Z Vet J. 2015;63:199\u2013210. https:\/\/doi.org\/10.1080\/00480169.2014.960500.","journal-title":"N Z Vet J"},{"key":"6804_CR8","doi-asserted-by":"publisher","first-page":"879","DOI":"10.1016\/j.ijpara.2014.08.009","volume":"44","author":"RM Sandeman","year":"2014","unstructured":"Sandeman RM, Levot GW, Heath ACG, James PJ, Greeff JC, Scott MJ, et al. Control of the sheep blowfly in Australia and New Zealand\u2014are we there yet? Int J Parasitol. 2014;44:879\u201391.","journal-title":"Int J Parasitol"},{"key":"6804_CR9","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.ibmb.2014.06.001","volume":"51","author":"F Li","year":"2014","unstructured":"Li F, Wantuch HA, Linger RJ, Belikoff EJ, Scott MJ. Transgenic sexing system for genetic control of the Australian sheep blow fly Lucilia cuprina. Insect Biochem Mol Biol. 2014;51:80\u20138.","journal-title":"Insect Biochem Mol Biol"},{"key":"6804_CR10","doi-asserted-by":"publisher","first-page":"16090","DOI":"10.1038\/srep16090","volume":"5","author":"Y Yan","year":"2015","unstructured":"Yan Y, Scott MJ. A transgenic embryonic sexing system for the Australian sheep blow fly Lucilia cuprina. Sci Rep. 2015;5:16090.","journal-title":"Sci Rep"},{"key":"6804_CR11","doi-asserted-by":"publisher","DOI":"10.4324\/9781351163767","volume-title":"Forensic entomology: the utility of arthropods in legal investigations","author":"JH Byrd","year":"2019","unstructured":"Byrd JH, Tomberlin JK. Forensic entomology: the utility of arthropods in legal investigations. Boca Raton: CRC Press; 2019."},{"key":"6804_CR12","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/S0304-4017(99)00047-3","volume":"83","author":"A Fenton","year":"1999","unstructured":"Fenton A, Wall R, French NP. The effects of oviposition aggregation on the incidence of sheep blowfly strike. Vet Parasitol. 1999;83:137\u201350.","journal-title":"Vet Parasitol"},{"key":"6804_CR13","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1111\/j.1365-2915.1994.tb00093.x","volume":"8","author":"JR Ashworth","year":"1994","unstructured":"Ashworth JR, Wall R. Responses of the sheep blowflies Lucilia sericata and L. cuprina to odour and the development of semiochemical baits. Med Vet Entomol. 1994;8:303\u20139. https:\/\/doi.org\/10.1111\/j.1365-2915.1994.tb00093.x.","journal-title":"Med Vet Entomol"},{"key":"6804_CR14","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1038\/146268a0","volume":"146","author":"FG Lennox","year":"1940","unstructured":"Lennox FG. Distribution of ammonia in larv\u00e6 of Lucilia cuprina. Nature. 1940;146:268\u2013268.","journal-title":"Nature"},{"key":"6804_CR15","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1111\/j.1365-2583.1994.tb00168.x","volume":"3","author":"RE Casu","year":"1994","unstructured":"Casu RE, Pearson RD, Jarmey JM, Cadogan LC, Riding GA, Tellam RL. Excretory\/secretory chymotrypsin from Lucilia cuprina: purification, enzymatic specificity and amino acid sequence deduced from mRNA. Insect Mol Biol. 1994;3:201\u201311. https:\/\/doi.org\/10.1111\/j.1365-2583.1994.tb00168.x.","journal-title":"Insect Mol Biol"},{"key":"6804_CR16","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1186\/1476-0711-10-6","volume":"10","author":"S Arora","year":"2011","unstructured":"Arora S, Baptista C, Lim CS. Maggot metabolites and their combinatory effects with antibiotic on Staphylococcus aureus. Ann Clin Microbiol Antimicrob. 2011;10:6.","journal-title":"Ann Clin Microbiol Antimicrob"},{"key":"6804_CR17","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1016\/j.jinsphys.2016.09.009","volume":"95","author":"Z Kotz\u00e9","year":"2016","unstructured":"Kotz\u00e9 Z, Villet MH, Weldon CW. Heat accumulation and development rate of massed maggots of the sheep blowfly, Lucilia cuprina (Diptera: Calliphoridae). J Insect Physiol. 2016;95:98\u2013104.","journal-title":"J Insect Physiol"},{"key":"6804_CR18","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1007\/s00414-015-1153-y","volume":"129","author":"Z Kotz\u00e9","year":"2015","unstructured":"Kotz\u00e9 Z, Villet MH, Weldon CW. Effect of temperature on development of the blowfly, Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae). Int J Legal Med. 2015;129:1155\u201362.","journal-title":"Int J Legal Med"},{"key":"6804_CR19","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1093\/jme\/tjx211","volume":"55","author":"YTB Bambaradeniya","year":"2018","unstructured":"Bambaradeniya YTB, Karunaratne WAIP, Tomberlin JK, Goonerathne I, Kotakadeniya RB. Temperature and tissue type impact development of Lucilia cuprina (Diptera: Calliphoridae) in Sri Lanka. J Med Entomol. 2018;55:285\u201391.","journal-title":"J Med Entomol"},{"key":"6804_CR20","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/avj.13131","volume":"100","author":"A Kotze","year":"2022","unstructured":"Kotze A, James P. Control of sheep flystrike: what\u2019s been tried in the past and where to from here. Aust Vet J. 2022;100:1\u201319. https:\/\/doi.org\/10.1111\/avj.13131.","journal-title":"Aust Vet J"},{"key":"6804_CR21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0095107","volume":"9","author":"SL Forbes","year":"2014","unstructured":"Forbes SL, Perrault KA. Decomposition odour profiling in the air and soil surrounding vertebrate carrion. PLoS ONE. 2014;9:e95107. https:\/\/doi.org\/10.1371\/journal.pone.0095107.","journal-title":"PLoS ONE"},{"key":"6804_CR22","doi-asserted-by":"publisher","first-page":"1407","DOI":"10.1007\/s10886-013-0359-z","volume":"39","author":"JJ Zhu","year":"2013","unstructured":"Zhu JJ, Chaudhury MF, Tangtrakulwanich K, Skoda SR. Identification of oviposition attractants of the secondary screwworm, Cochliomyia macellaria (F.) released from rotten chicken liver. J Chem Ecol. 2013;39:1407\u201314. https:\/\/doi.org\/10.1007\/s10886-013-0359-z.","journal-title":"J Chem Ecol"},{"key":"6804_CR23","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1093\/jmedent\/47.3.349","volume":"47","author":"MF Chaudhury","year":"2010","unstructured":"Chaudhury MF, Skoda SR, Sagel A, Welch JB. Volatiles emitted from eight wound-isolated bacteria differentially attract gravid screwworms (Diptera: Calliphoridae) to oviposit. J Med Entomol. 2010;47:349\u201354.","journal-title":"J Med Entomol"},{"key":"6804_CR24","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/j.anbehav.2016.03.022","volume":"115","author":"W Liu","year":"2016","unstructured":"Liu W, Longnecker M, Tarone AM, Tomberlin JK. Responses of Lucilia sericata (Diptera: Calliphoridae) to compounds from microbial decomposition of larval resources. Anim Behav. 2016;115:217\u201325.","journal-title":"Anim Behav"},{"key":"6804_CR25","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1186\/s13071-024-06391-6","volume":"17","author":"JP Wulff","year":"2024","unstructured":"Wulff JP, Hickner PV, Watson DW, Denning SS, Belikoff EJ, Scott MJ. Antennal transcriptome analysis reveals sensory receptors potentially associated with host detection in the livestock pest Lucilia cuprina. Parasit Vectors. 2024;17:308. https:\/\/doi.org\/10.1186\/s13071-024-06391-6.","journal-title":"Parasit Vectors"},{"key":"6804_CR26","doi-asserted-by":"publisher","DOI":"10.3791\/65835","volume":"201","author":"VAS Cunha","year":"2023","unstructured":"Cunha VAS, Tandonnet S, Ferreira DL, Rodrigues AV, Torres TT. Exploring life history choices: using temperature and substrate type as interacting factors for blowfly larval and female preferences. J Vis Exp. 2023;201:e65835. https:\/\/doi.org\/10.3791\/65835.","journal-title":"J Vis Exp"},{"key":"6804_CR27","doi-asserted-by":"publisher","first-page":"1109","DOI":"10.1603\/033.046.0518","volume":"46","author":"FD Guerrero","year":"2009","unstructured":"Guerrero FD, Dowd SE, Djikeng A, Wiley G, Macmil S, Saldivar L, et al. A database of expressed genes from Cochliomyia hominivorax (Diptera: Calliphoridae). J Med Entomol. 2009;46:1109\u201316. https:\/\/doi.org\/10.1603\/033.046.0518.","journal-title":"J Med Entomol"},{"key":"6804_CR28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0063168","volume":"8","author":"M Zhang","year":"2013","unstructured":"Zhang M, Yu H, Yang Y, Song C, Hu X, Zhang G. Analysis of the transcriptome of blowfly Chrysomya megacephala (Fabricius) larvae in responses to different edible oils. PLoS ONE. 2013;8:e63168. https:\/\/doi.org\/10.1371\/journal.pone.0063168.","journal-title":"PLoS ONE"},{"key":"6804_CR29","doi-asserted-by":"publisher","first-page":"948","DOI":"10.3390\/insects13100948","volume":"13","author":"CE Hjelmen","year":"2022","unstructured":"Hjelmen CE, Yuan Y, Parrott JJ, McGuane AS, Srivastav SP, Purcell AC, et al. Identification and characterization of small RNA markers of age in the blow fly Cochliomyia macellaria (Fabricius) (Diptera: Calliphoridae). Insects. 2022;13:948.","journal-title":"Insects"},{"key":"6804_CR30","doi-asserted-by":"publisher","first-page":"3978","DOI":"10.1016\/j.ygeno.2021.10.003","volume":"113","author":"RJ Davis","year":"2021","unstructured":"Davis RJ, Belikoff EJ, Dickey AN, Scholl EH, Benoit JB, Scott MJ. Genome and transcriptome sequencing of the green bottle fly, Lucilia sericata, reveals underlying factors of sheep flystrike and maggot debridement therapy. Genomics. 2021;113:3978\u201388.","journal-title":"Genomics"},{"key":"6804_CR31","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1186\/s12864-020-6509-0","volume":"21","author":"Z Liu","year":"2020","unstructured":"Liu Z, Han H, Meng F, Jiang Y, Cai J. Dynamic transcriptome profiling exploring cold tolerance in forensically important blow fly, Aldrichina grahami (Diptera: Calliphoridae). BMC Genom. 2020;21:92.","journal-title":"BMC Genom"},{"key":"6804_CR32","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1111\/imb.12740","volume":"31","author":"CH McKenna","year":"2022","unstructured":"McKenna CH, Asgari D, Crippen TL, Zheng L, Sherman RA, Tomberlin JK, et al. Gene expression in Lucilia sericata (Diptera: Calliphoridae) larvae exposed to Pseudomonas aeruginosa and Acinetobacter baumannii identifies shared and microbe-specific induction of immune genes. Insect Mol Biol. 2022;31:85\u2013100. https:\/\/doi.org\/10.1111\/imb.12740.","journal-title":"Insect Mol Biol"},{"key":"6804_CR33","doi-asserted-by":"publisher","first-page":"2086","DOI":"10.1016\/j.cub.2005.11.016","volume":"15","author":"E Fishilevich","year":"2005","unstructured":"Fishilevich E, Domingos AI, Asahina K, Naef F, Vosshall LB, Louis M. Chemotaxis behavior mediated by single larval olfactory neurons in Drosophila. Curr Biol. 2005;15:2086\u201396.","journal-title":"Curr Biol"},{"key":"6804_CR34","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1093\/chemse\/bjl030","volume":"32","author":"B Gerber","year":"2007","unstructured":"Gerber B, Stocker RF. The Drosophila larva as a model for studying chemosensation and chemosensory learning: a review. Chem Sens. 2007;32:65\u201389. https:\/\/doi.org\/10.1093\/chemse\/bjl030.","journal-title":"Chem Sens"},{"key":"6804_CR35","doi-asserted-by":"publisher","DOI":"10.3389\/fnbeh.2014.00038","volume":"8","author":"A Gomez-Marin","year":"2014","unstructured":"Gomez-Marin A, Louis M. Multilevel control of run orientation in Drosophila larval chemotaxis. Front Behav Neurosci. 2014;8:38. https:\/\/doi.org\/10.3389\/fnbeh.2014.00038.","journal-title":"Front Behav Neurosci"},{"key":"6804_CR36","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1038\/ncomms1455","volume":"2","author":"A Gomez-Marin","year":"2011","unstructured":"Gomez-Marin A, Stephens GJ, Louis M. Active sampling and decision making in Drosophila chemotaxis. Nat Commun. 2011;2:441.","journal-title":"Nat Commun"},{"key":"6804_CR37","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1038\/nn2031","volume":"11","author":"M Louis","year":"2008","unstructured":"Louis M, Huber T, Benton R, Sakmar TP, Vosshall LB. Bilateral olfactory sensory input enhances chemotaxis behavior. Nat Neurosci. 2008;11:187\u201399.","journal-title":"Nat Neurosci"},{"key":"6804_CR38","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1016\/j.neuron.2008.06.010","volume":"59","author":"SA Kreher","year":"2008","unstructured":"Kreher SA, Mathew D, Kim J, Carlson JR. Translation of sensory input into behavioral output via an olfactory system. Neuron. 2008;59:110\u201324.","journal-title":"Neuron"},{"key":"6804_CR39","doi-asserted-by":"publisher","first-page":"20160665","DOI":"10.1098\/rspb.2016.0665","volume":"283","author":"M Grillet","year":"2016","unstructured":"Grillet M, Campagner D, Petersen R, McCrohan C, Cobb M. The peripheral olfactory code in Drosophila larvae contains temporal information and is robust over multiple timescales. Proc Royal Soc B Biol Sci. 2016;283:20160665. https:\/\/doi.org\/10.1098\/rspb.2016.0665.","journal-title":"Proc Royal Soc B Biol Sci"},{"key":"6804_CR40","doi-asserted-by":"publisher","first-page":"14110","DOI":"10.1073\/pnas.0702421104","volume":"104","author":"Y Jiao","year":"2007","unstructured":"Jiao Y, Moon SJ, Montell C. A Drosophila gustatory receptor required for the responses to sucrose, glucose, and maltose identified by mRNA tagging. Proc Natl Acad Sci. 2007;104:14110\u20135. https:\/\/doi.org\/10.1073\/pnas.0702421104.","journal-title":"Proc Natl Acad Sci"},{"key":"6804_CR41","doi-asserted-by":"publisher","first-page":"580","DOI":"10.1038\/nature12390","volume":"500","author":"L Ni","year":"2013","unstructured":"Ni L, Bronk P, Chang EC, Lowell AM, Flam JO, Panzano VC, et al. A gustatory receptor paralogue controls rapid warmth avoidance in Drosophila. Nature. 2013;500:580\u20134.","journal-title":"Nature"},{"key":"6804_CR42","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201847328","volume":"20","author":"J Sang","year":"2019","unstructured":"Sang J, Rimal S, Lee Y. Gustatory receptor 28b is necessary for avoiding saponin in Drosophila melanogaster. EMBO Rep. 2019;20:e47328. https:\/\/doi.org\/10.15252\/embr.201847328.","journal-title":"EMBO Rep"},{"key":"6804_CR43","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.13254","volume":"5","author":"L Ni","year":"2016","unstructured":"Ni L, Klein M, Svec KV, Budelli G, Chang EC, Ferrer AJ, et al. The ionotropic receptors IR21a and IR25a mediate cool sensing in Drosophila. eLife. 2016;5:e13254. https:\/\/doi.org\/10.7554\/eLife.13254.","journal-title":"eLife"},{"key":"6804_CR44","doi-asserted-by":"publisher","first-page":"4195","DOI":"10.1073\/pnas.1503292112","volume":"112","author":"S Stewart","year":"2015","unstructured":"Stewart S, Koh T-W, Ghosh AC, Carlson JR. Candidate ionotropic taste receptors in the Drosophila larva. Proc Natl Acad Sci. 2015;112:4195\u2013201. https:\/\/doi.org\/10.1073\/pnas.1503292112.","journal-title":"Proc Natl Acad Sci"},{"key":"6804_CR45","doi-asserted-by":"publisher","first-page":"1557","DOI":"10.1016\/S0960-9822(03)00596-7","volume":"13","author":"JA Ainsley","year":"2003","unstructured":"Ainsley JA, Pettus JM, Bosenko D, Gerstein CE, Zinkevich N, Anderson MG, et al. Enhanced locomotion caused by loss of the Drosophila DEG\/ENaC protein Pickpocket1. Curr Biol. 2003;13:1557\u201363.","journal-title":"Curr Biol"},{"key":"6804_CR46","doi-asserted-by":"publisher","first-page":"E220","DOI":"10.1073\/pnas.1416212112","volume":"112","author":"M Klein","year":"2015","unstructured":"Klein M, Afonso B, Vonner AJ, Hernandez-Nunez L, Berck M, Tabone CJ, et al. Sensory determinants of behavioral dynamics in Drosophila thermotaxis. Proc Natl Acad Sci. 2015;112:E220\u20139. https:\/\/doi.org\/10.1073\/pnas.1416212112.","journal-title":"Proc Natl Acad Sci"},{"key":"6804_CR47","doi-asserted-by":"publisher","first-page":"10465","DOI":"10.1523\/JNEUROSCI.1631-10.2010","volume":"30","author":"Y Kwon","year":"2010","unstructured":"Kwon Y, Shen WL, Shim H-S, Montell C. Fine thermotactic discrimination between the optimal and slightly cooler temperatures via a TRPV channel in chordotonal neurons. J Neurosci. 2010;30:10465\u201371. https:\/\/doi.org\/10.1523\/JNEUROSCI.1631-10.2010.","journal-title":"J Neurosci"},{"key":"6804_CR48","doi-asserted-by":"publisher","first-page":"2229","DOI":"10.1126\/science.287.5461.2229","volume":"287","author":"RG Walker","year":"2000","unstructured":"Walker RG, Willingham AT, Zuker CS. A Drosophila mechanosensory transduction channel (1979). Science. 2000;287:2229\u201334. https:\/\/doi.org\/10.1126\/science.287.5461.2229.","journal-title":"Science"},{"key":"6804_CR49","doi-asserted-by":"publisher","first-page":"3075","DOI":"10.1152\/jn.00071.2009","volume":"101","author":"SR Pulver","year":"2009","unstructured":"Pulver SR, Pashkovski SL, Hornstein NJ, Garrity PA, Griffith LC. Temporal dynamics of neuronal activation by channelrhodopsin-2 and TRPA1 determine behavioral output in Drosophila Larvae. J Neurophysiol. 2009;101:3075\u201388. https:\/\/doi.org\/10.1152\/jn.00071.2009.","journal-title":"J Neurophysiol"},{"key":"6804_CR50","doi-asserted-by":"publisher","first-page":"509","DOI":"10.3390\/biom11040509","volume":"11","author":"K Rihani","year":"2021","unstructured":"Rihani K, Ferveur J-F, Briand L. The 40-year mystery of insect odorant-binding proteins. Biomolecules. 2021;11:509.","journal-title":"Biomolecules"},{"key":"6804_CR51","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1186\/s12864-015-1466-8","volume":"16","author":"O Leitch","year":"2015","unstructured":"Leitch O, Papanicolaou A, Lennard C, Kirkbride KP, Anderson A. Chemosensory genes identified in the antennal transcriptome of the blowfly Calliphora stygia. BMC Genom. 2015;16:255. https:\/\/doi.org\/10.1186\/s12864-015-1466-8.","journal-title":"BMC Genom"},{"key":"6804_CR52","doi-asserted-by":"publisher","first-page":"3382","DOI":"10.1016\/j.cub.2021.05.025","volume":"31","author":"A Vulpe","year":"2021","unstructured":"Vulpe A, Kim HS, Ballou S, Wu S-T, Grabe V, Nava Gonzales C, et al. An ammonium transporter is a non-canonical olfactory receptor for ammonia. Curr Biol. 2021;31:3382-3390.e7.","journal-title":"Curr Biol"},{"key":"6804_CR53","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2022.957247\/full","author":"JJ Rond\u00f3n","year":"2022","unstructured":"Rond\u00f3n JJ, Moreyra NN, Pisarenco VA, Rozas J, Hurtado J, Hasson E. Evolution of the odorant-binding protein gene family in Drosophila. Front Ecol Evol. 2022;10:957247. https:\/\/doi.org\/10.3389\/fevo.2022.957247\/full.","journal-title":"Front Ecol Evol"},{"key":"6804_CR54","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2018.00496\/full","volume":"12","author":"D Wicher","year":"2018","unstructured":"Wicher D, Marion-Poll F. Editorial: function and regulation of chemoreceptors. Front Cell Neurosci. 2018;12:496. https:\/\/doi.org\/10.3389\/fncel.2018.00496\/full.","journal-title":"Front Cell Neurosci"},{"key":"6804_CR55","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1009792","volume":"17","author":"ME Williamson","year":"2021","unstructured":"Williamson ME, Yan Y, Scott MJ. Conditional knockdown of transformer in sheep blow fly suggests a role in repression of dosage compensation and potential for population suppression. PLoS Genet. 2021;17:e1009792.","journal-title":"PLoS Genet"},{"key":"6804_CR56","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1016\/j.ibmb.2010.09.006","volume":"41","author":"C Concha","year":"2011","unstructured":"Concha C, Belikoff EJ, Carey B, Li F, Schiemann AH, Scott MJ. Efficient germ-line transformation of the economically important pest species Lucilia cuprina and Lucilia sericata (Diptera, Calliphoridae). Insect Biochem Mol Biol. 2011;41:70\u20135.","journal-title":"Insect Biochem Mol Biol"},{"key":"6804_CR57","doi-asserted-by":"publisher","DOI":"10.1242\/dev.193854","volume":"147","author":"C Cockrum","year":"2020","unstructured":"Cockrum C, Kaneshiro KR, Rechtsteiner A, Tabuchi TM, Strome S. A primer for generating and using transcriptome data and gene sets. Development. 2020;147:dev193854. https:\/\/doi.org\/10.1242\/dev.193854.","journal-title":"Development"},{"key":"6804_CR58","doi-asserted-by":"publisher","first-page":"1","DOI":"10.18637\/jss.v025.i01","volume":"25","author":"S L\u00ea","year":"2008","unstructured":"L\u00ea S, Josse J, Husson F. FactoMineR: an R package for multivariate analysis. J Stat Softw. 2008;25:1\u20138.","journal-title":"J Stat Softw"},{"key":"6804_CR59","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v035.b01","volume":"35","author":"PM Valero-Mora","year":"2010","unstructured":"Valero-Mora PM. ggplot2: elegant graphics for data analysis. J Stat Softw Bk Rev. 2010;35:1. https:\/\/doi.org\/10.18637\/jss.v035.b01.","journal-title":"J Stat Softw Bk Rev"},{"key":"6804_CR60","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8","volume":"15","author":"MI Love","year":"2014","unstructured":"Love MI, Huber W, Anders S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014;15:550. https:\/\/doi.org\/10.1186\/s13059-014-0550-8.","journal-title":"Genome Biol"},{"key":"6804_CR61","doi-asserted-by":"publisher","first-page":"738088","DOI":"10.3389\/fnins.2021.738088","volume":"15","author":"K Mika","year":"2021","unstructured":"Mika K, Benton R. Olfactory receptor gene regulation in insects: multiple mechanisms for singular expression. Front Neurosci. 2021;15:738088.","journal-title":"Front Neurosci"},{"key":"6804_CR62","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1093\/molbev\/mst010","volume":"30","author":"K Katoh","year":"2013","unstructured":"Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol. 2013;30:772\u201380.","journal-title":"Mol Biol Evol"},{"key":"6804_CR63","first-page":"406","volume":"4","author":"N Saitou","year":"1987","unstructured":"Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4:406.","journal-title":"Mol Biol Evol"},{"key":"6804_CR64","doi-asserted-by":"publisher","first-page":"3022","DOI":"10.1093\/molbev\/msab120","volume":"38","author":"K Tamura","year":"2021","unstructured":"Tamura K, Stecher G, Kumar S. MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol. 2021;38:3022\u20137.","journal-title":"Mol Biol Evol"},{"key":"6804_CR65","doi-asserted-by":"publisher","first-page":"11379","DOI":"10.1038\/s41598-021-90649-x","volume":"11","author":"DF Paulo","year":"2021","unstructured":"Paulo DF, Junqueira ACM, Arp AP, Vieira AS, Ceballos J, Skoda SR, et al. Disruption of the odorant coreceptor Orco impairs foraging and host finding behaviors in the New World screwworm fly. Sci Rep. 2021;11:11379.","journal-title":"Sci Rep"},{"key":"6804_CR66","doi-asserted-by":"publisher","first-page":"2782","DOI":"10.1093\/bioinformatics\/bts515","volume":"28","author":"J Feng","year":"2012","unstructured":"Feng J, Meyer CA, Wang Q, Liu JS, Shirley Liu X, Zhang Y. GFOLD: a generalized fold change for ranking differentially expressed genes from RNA-seq data. Bioinformatics. 2012;28:2782\u20138.","journal-title":"Bioinformatics"},{"key":"6804_CR67","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.72599","volume":"11","author":"D Task","year":"2022","unstructured":"Task D, Lin C-C, Vulpe A, Afify A, Ballou S, Brbic M, et al. Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons. eLife. 2022;11:e72599. https:\/\/doi.org\/10.7554\/eLife.72599.","journal-title":"eLife"},{"key":"6804_CR68","doi-asserted-by":"publisher","first-page":"8580","DOI":"10.1038\/s41598-018-26825-3","volume":"8","author":"N Utashiro","year":"2018","unstructured":"Utashiro N, Williams CR, Parrish JZ, Emoto K. Prior activity of olfactory receptor neurons is required for proper sensory processing and behavior in Drosophila larvae. Sci Rep. 2018;8:8580.","journal-title":"Sci Rep"},{"key":"6804_CR69","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.ibmb.2013.11.001","volume":"45","author":"W Dermauw","year":"2014","unstructured":"Dermauw W, Van Leeuwen T. The ABC gene family in arthropods: comparative genomics and role in insecticide transport and resistance. Insect Biochem Mol Biol. 2014;45:89\u2013110.","journal-title":"Insect Biochem Mol Biol"},{"key":"6804_CR70","doi-asserted-by":"publisher","first-page":"1127","DOI":"10.3390\/pathogens10091127","volume":"10","author":"V Kumar","year":"2021","unstructured":"Kumar V, Garg S, Gupta L, Gupta K, Diagne CT, Miss\u00e9 D, et al. Delineating the role of Aedes aegypti ABC transporter gene family during mosquito development and arboviral infection via transcriptome analyses. Pathogens. 2021;10:1127.","journal-title":"Pathogens"},{"key":"6804_CR71","doi-asserted-by":"publisher","first-page":"2829","DOI":"10.3390\/ijms20112829","volume":"20","author":"C Wu","year":"2019","unstructured":"Wu C, Chakrabarty S, Jin M, Liu K, Xiao Y. Insect ATP-binding cassette (ABC) transporters: roles in xenobiotic detoxification and Bt insecticidal activity. Int J Mol Sci. 2019;20:2829.","journal-title":"Int J Mol Sci"},{"key":"6804_CR72","doi-asserted-by":"publisher","first-page":"14752","DOI":"10.1074\/jbc.M701361200","volume":"282","author":"DS Andersen","year":"2007","unstructured":"Andersen DS, Leevers SJ. The essential Drosophila ATP-binding cassette domain protein, pixie, binds the 40 S ribosome in an ATP-dependent manner and is required for translation initiation. J Biol Chem. 2007;282:14752\u201360.","journal-title":"J Biol Chem"},{"key":"6804_CR73","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1002\/1873-3468.13984","volume":"595","author":"CR Fostier","year":"2021","unstructured":"Fostier CR, Monlezun L, Ousalem F, Singh S, Hunt JF, Bo\u00ebl G. ABC-F translation factors: from antibiotic resistance to immune response. FEBS Lett. 2021;595:675\u2013706. https:\/\/doi.org\/10.1002\/1873-3468.13984.","journal-title":"FEBS Lett"},{"key":"6804_CR74","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008363","volume":"16","author":"Y Wang","year":"2020","unstructured":"Wang Y, Norum M, Oehl K, Yang Y, Zuber R, Yang J, et al. Dysfunction of Oskyddad causes Harlequin-type ichthyosis-like defects in Drosophila melanogaster. PLoS Genet. 2020;16:e1008363. https:\/\/doi.org\/10.1371\/journal.pgen.1008363.","journal-title":"PLoS Genet"},{"key":"6804_CR75","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.ejcb.2017.12.003","volume":"97","author":"R Zuber","year":"2018","unstructured":"Zuber R, Norum M, Wang Y, Oehl K, Gehring N, Accardi D, et al. The ABC transporter Snu and the extracellular protein Snsl cooperate in the formation of the lipid-based inward and outward barrier in the skin of Drosophila. Eur J Cell Biol. 2018;97:90\u2013101.","journal-title":"Eur J Cell Biol"},{"key":"6804_CR76","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1016\/j.ibmb.2004.03.009","volume":"34","author":"GE Abo-Elghar","year":"2004","unstructured":"Abo-Elghar GE, Fujiyoshi P, Matsumura F. Significance of the sulfonylurea receptor (SUR) as the target of diflubenzuron in chitin synthesis inhibition in Drosophila melanogaster and Blattella germanica. Insect Biochem Mol Biol. 2004;34:743\u201352.","journal-title":"Insect Biochem Mol Biol"},{"key":"6804_CR77","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.01134\/full","volume":"8","author":"SR Whiten","year":"2018","unstructured":"Whiten SR, Eggleston H, Adelman ZN. Ironing out the details: exploring the role of iron and heme in blood-sucking arthropods. Front Physiol. 2018;8:1134. https:\/\/doi.org\/10.3389\/fphys.2017.01134\/full.","journal-title":"Front Physiol"},{"key":"6804_CR78","doi-asserted-by":"publisher","DOI":"10.52707\/1081-1710-48.2.72","author":"GSh Selem","year":"2023","unstructured":"Selem GSh, Geden CJ, Khater H, Khater KS. Effects of larval diets on some biological parameters and morphometric and biochemical analysis of ovaries of Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae). J Vector Ecol. 2023. https:\/\/doi.org\/10.52707\/1081-1710-48.2.72.","journal-title":"J Vector Ecol"},{"key":"6804_CR79","doi-asserted-by":"publisher","first-page":"20771","DOI":"10.1038\/s41598-020-77541-w","volume":"10","author":"PV Hickner","year":"2020","unstructured":"Hickner PV, Mittapalli O, Subramoniam A, Sagel A, Watson W, Scott MJ, et al. Physiological and molecular correlates of the screwworm fly attraction to wound and animal odors. Sci Rep. 2020;10:20771.","journal-title":"Sci Rep"},{"key":"6804_CR80","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000681","volume":"5","author":"S Zhou","year":"2009","unstructured":"Zhou S, Stone EA, Mackay TFC, Anholt RRH. Plasticity of the chemoreceptor repertoire in Drosophila melanogaster. PLoS Genet. 2009;5:e1000681.","journal-title":"PLoS Genet"},{"key":"6804_CR81","doi-asserted-by":"publisher","first-page":"3335","DOI":"10.1534\/g3.116.034595","volume":"6","author":"JR Shorter","year":"2016","unstructured":"Shorter JR, Dembeck LM, Everett LJ, Morozova TV, Arya GH, Turlapati L, et al. Obp56h modulates mating behavior in Drosophila melanogaster. G3 Genes Genom, Genet. 2016;6:3335\u201342.","journal-title":"G3 Genes Genom, Genet"},{"key":"6804_CR82","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1016\/0305-0491(88)90305-7","volume":"89","author":"KH Lockey","year":"1988","unstructured":"Lockey KH. Lipids of the insect cuticle: origin, composition and function. Comp Biochem Physiol Part B Comp Biochem. 1988;89:595\u2013645.","journal-title":"Comp Biochem Physiol Part B Comp Biochem"},{"key":"6804_CR83","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.00819\/full","volume":"11","author":"Y Zhang","year":"2020","unstructured":"Zhang Y, Gao S, Xue S, Zhang K, Wang J, Li B. Odorant-binding proteins contribute to the defense of the red flour beetle, Tribolium castaneum, against essential oil of Artemisia vulgaris. Front Physiol. 2020;11:818. https:\/\/doi.org\/10.3389\/fphys.2020.00819\/full.","journal-title":"Front Physiol"},{"key":"6804_CR84","doi-asserted-by":"publisher","DOI":"10.3389\/finsc.2023.1274197\/full","volume":"3","author":"JA Abendroth","year":"2023","unstructured":"Abendroth JA, Moural TW, Wei H, Zhu F. Roles of insect odorant binding proteins in communication and xenobiotic adaptation. Front Insect Sci. 2023;3:1274197. https:\/\/doi.org\/10.3389\/finsc.2023.1274197\/full.","journal-title":"Front Insect Sci"},{"key":"6804_CR85","doi-asserted-by":"publisher","first-page":"22532","DOI":"10.1021\/acs.jafc.4c05737","volume":"72","author":"R Zhao","year":"2024","unstructured":"Zhao R, Wang H-H, Wang Z, Xiao X, Yin X-H, Hu S-Y, et al. Omics analysis of odorant-binding proteins and cuticle-enriched SfruOBP18 confers multi-insecticide tolerance in Spodoptera frugiperda. J Agric Food Chem. 2024;72:22532\u201344. https:\/\/doi.org\/10.1021\/acs.jafc.4c05737.","journal-title":"J Agric Food Chem"},{"key":"6804_CR86","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1007\/s12104-024-10178-2","volume":"18","author":"ML Cotten","year":"2024","unstructured":"Cotten ML, Starich MR, He Y, Yin J, Yuan Q, Tjandra N. NMR chemical shift assignment of Drosophila odorant binding protein 44a in complex with 8(Z)-eicosenoic acid. Biomol NMR Assign. 2024;18:129\u201334. https:\/\/doi.org\/10.1007\/s12104-024-10178-2.","journal-title":"Biomol NMR Assign"},{"key":"6804_CR87","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1016\/j.neuron.2013.06.025","volume":"79","author":"YT Jeong","year":"2013","unstructured":"Jeong YT, Shim J, Oh SR, Yoon HI, Kim CH, Moon SJ, et al. An odorant-binding protein required for suppression of sweet taste by bitter chemicals. Neuron. 2013;79:725\u201337.","journal-title":"Neuron"},{"key":"6804_CR88","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1093\/jisesa\/iew008","volume":"16","author":"A Gonz\u00e1lez-Gonz\u00e1lez","year":"2016","unstructured":"Gonz\u00e1lez-Gonz\u00e1lez A, Palma-Millanao R, Y\u00e1\u00f1ez O, Rojas M, Mutis A, Venthur H, et al. Virtual screening of plant volatile compounds reveals a high affinity of Hylamorpha elegans (Coleoptera: Scarabaeidae) odorant-binding proteins for sesquiterpenes from its native host. J Insect Sci. 2016;16:30. https:\/\/doi.org\/10.1093\/jisesa\/iew008.","journal-title":"J Insect Sci"},{"key":"6804_CR89","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.66741","volume":"10","author":"D Giordano","year":"2021","unstructured":"Giordano D, Facchiano A, D\u2019Auria S, Loreto F. A hypothesis on the capacity of plant odorant-binding proteins to bind volatile isoprenoids based on in silico evidences. eLife. 2021;10:e66741. https:\/\/doi.org\/10.7554\/eLife.66741.","journal-title":"eLife"},{"key":"6804_CR90","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1016\/j.ibmb.2009.03.004","volume":"39","author":"H Qiao","year":"2009","unstructured":"Qiao H, Tuccori E, He X, Gazzano A, Field L, Zhou J-J, et al. Discrimination of alarm pheromone (E)-\u03b2-farnesene by aphid odorant-binding proteins. Insect Biochem Mol Biol. 2009;39:414\u20139.","journal-title":"Insect Biochem Mol Biol"},{"key":"6804_CR91","doi-asserted-by":"publisher","first-page":"11677","DOI":"10.1038\/s41598-017-11199-9","volume":"7","author":"MM Rahman","year":"2017","unstructured":"Rahman MM, Franch-Marro X, Maestro JL, Martin D, Casali A. Local Juvenile Hormone activity regulates gut homeostasis and tumor growth in adult Drosophila. Sci Rep. 2017;7:11677.","journal-title":"Sci Rep"},{"key":"6804_CR92","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1038\/s42003-024-06334-y","volume":"7","author":"ML Taracena-Agarwal","year":"2024","unstructured":"Taracena-Agarwal ML, Walter-Nuno AB, Bottino-Rojas V, Mejia APG, Xu K, Segal S, et al. Juvenile Hormone as a contributing factor in establishing midgut microbiota for fecundity and fitness enhancement in adult female Aedes aegypti. Commun Biol. 2024;7:687.","journal-title":"Commun Biol"},{"key":"6804_CR93","doi-asserted-by":"publisher","first-page":"170","DOI":"10.4161\/cib.7708","volume":"2","author":"T Sokabe","year":"2009","unstructured":"Sokabe T, Tominaga M. A temperature-sensitive TRP ion channel, painless, functions as a noxious heat sensor in fruit flies. Commun Integr Biol. 2009;2:170\u20133. https:\/\/doi.org\/10.4161\/cib.7708.","journal-title":"Commun Integr Biol"},{"key":"6804_CR94","doi-asserted-by":"publisher","first-page":"2042","DOI":"10.1038\/ncomms3042","volume":"4","author":"S Charlu","year":"2013","unstructured":"Charlu S, Wisotsky Z, Medina A, Dahanukar A. Acid sensing by sweet and bitter taste neurons in Drosophila melanogaster. Nat Commun. 2013;4:2042.","journal-title":"Nat Commun"},{"key":"6804_CR95","doi-asserted-by":"publisher","first-page":"1034","DOI":"10.1016\/j.cub.2006.04.002","volume":"16","author":"B Al-Anzi","year":"2006","unstructured":"Al-Anzi B, Tracey WD, Benzer S. Response of Drosophila to Wasabi Is Mediated by painless, the Fly Homolog of Mammalian TRPA1\/ANKTM1. Curr Biol. 2006;16:1034\u201340.","journal-title":"Curr Biol"},{"key":"6804_CR96","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nn.2119","volume":"11","author":"J Xu","year":"2008","unstructured":"Xu J, Sornborger AT, Lee JK, Shen P. Drosophila TRPA channel modulates sugar-stimulated neural excitation, avoidance and social response. Nat Neurosci. 2008;11:676\u201382.","journal-title":"Nat Neurosci"},{"key":"6804_CR97","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1387\/ijdb.103254lh","volume":"55","author":"L Herboso","year":"2011","unstructured":"Herboso L, Talamillo A, Perez C, Barrio R. Expression of the scavenger receptor class B type I (SR-BI) family in Drosophila melanogaster. Int J Dev Biol. 2011;55:603\u201311.","journal-title":"Int J Dev Biol"},{"key":"6804_CR98","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1016\/j.ibmb.2012.04.002","volume":"42","author":"X-Z Shi","year":"2012","unstructured":"Shi X-Z, Zhong X, Yu X-Q. Drosophila melanogaster NPC2 proteins bind bacterial cell wall components and may function in immune signal pathways. Insect Biochem Mol Biol. 2012;42:545\u201356.","journal-title":"Insect Biochem Mol Biol"},{"key":"6804_CR99","doi-asserted-by":"publisher","DOI":"10.1093\/jisesa\/ieaa033\/5836191","volume":"20","author":"M-M Chen","year":"2020","unstructured":"Chen M-M, Zhong L, Zhao C-S, Wang F-C, Ji W-J, Zhang B, et al. Characterization of an ecdysteroid-regulated 16 kDa protein gene in chinese oak silkworm, Antheraea pernyi (Lepidoptera: Saturniidae). J Insect Sci. 2020;20:4. https:\/\/doi.org\/10.1093\/jisesa\/ieaa033\/5836191.","journal-title":"J Insect Sci"},{"key":"6804_CR100","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1186\/s12864-024-10034-6","volume":"25","author":"JP Wulff","year":"2024","unstructured":"Wulff JP, Traverso LM, Latorre-Estivalis JM, Segura DF, Lanzavecchia SB. Identification of candidate genes associated with host-seeking behavior in the parasitoid wasp Diachasmimorpha longicaudata. BMC Genomics. 2024;25:147. https:\/\/doi.org\/10.1186\/s12864-024-10034-6.","journal-title":"BMC Genomics"},{"key":"6804_CR101","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.989601\/full","volume":"13","author":"Y Yang","year":"2022","unstructured":"Yang Y, Hua D, Zhu J, Wang F, Zhang Y. Chemosensory protein 4 is required for Bradysia odoriphaga to be olfactory attracted to sulfur compounds released from Chinese chives. Front Physiol. 2022;13:989601. https:\/\/doi.org\/10.3389\/fphys.2022.989601\/full.","journal-title":"Front Physiol"},{"key":"6804_CR102","doi-asserted-by":"publisher","first-page":"43754","DOI":"10.1038\/srep43754","volume":"7","author":"R Delventhal","year":"2017","unstructured":"Delventhal R, Menuz K, Joseph R, Park J, Sun JS, Carlson JR. The taste response to ammonia in Drosophila. Sci Rep. 2017;7:43754.","journal-title":"Sci Rep"},{"key":"6804_CR103","first-page":"3818","volume":"216","author":"A Browne","year":"2013","unstructured":"Browne A, O\u2019Donnell MJ. Ammonium secretion by Malpighian tubules of Drosophila melanogaster: application of a novel ammonium-selective microelectrode. J Exp Biol. 2013;216:3818\u201327.","journal-title":"J Exp Biol"},{"key":"6804_CR104","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1007\/978-1-0716-2301-5_10","volume-title":"CRISPR\/Cas9 genome editing in the new world screwworm and Australian sheep blowfly","author":"DF Paulo","year":"2022","unstructured":"Paulo DF, Williamson ME, Scott MJ. CRISPR\/Cas9 genome editing in the new world screwworm and Australian sheep blowfly. Berlin: Springer; 2022. p. 173\u2013201. https:\/\/doi.org\/10.1007\/978-1-0716-2301-5_10."},{"key":"6804_CR105","doi-asserted-by":"publisher","first-page":"736","DOI":"10.1016\/j.cell.2017.06.051","volume":"170","author":"H Yan","year":"2017","unstructured":"Yan H, Opachaloemphan C, Mancini G, Yang H, Gallitto M, Mlejnek J, et al. An engineered Orco mutation produces aberrant social behavior and defective neural development in ants. Cell. 2017;170:736-747.e9.","journal-title":"Cell"},{"key":"6804_CR106","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1186\/s12915-022-01411-2","volume":"20","author":"X-B Fan","year":"2022","unstructured":"Fan X-B, Mo B-T, Li G-C, Huang L-Q, Guo H, Gong X-L, et al. Mutagenesis of the odorant receptor co-receptor (Orco) reveals severe olfactory defects in the crop pest moth Helicoverpa armigera. BMC Biol. 2022;20:214. https:\/\/doi.org\/10.1186\/s12915-022-01411-2.","journal-title":"BMC Biol"},{"key":"6804_CR107","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2020.00184\/full","volume":"8","author":"AM Lamb","year":"2020","unstructured":"Lamb AM, Wang Z, Simmer P, Chung H, Wittkopp PJ. ebony Affects pigmentation divergence and cuticular hydrocarbons in Drosophila Americana and D. novamexicana. Front Ecol Evol. 2020;8:184. https:\/\/doi.org\/10.3389\/fevo.2020.00184\/full.","journal-title":"Front Ecol Evol"},{"key":"6804_CR108","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1002\/neu.10196","volume":"55","author":"MD Drapeau","year":"2003","unstructured":"Drapeau MD, Radovic A, Wittkopp PJ, Long AD. A gene necessary for normal male courtship, yellow, acts downstream of fruitless in the Drosophila melanogaster larval brain. J Neurobiol. 2003;55:53\u201372. https:\/\/doi.org\/10.1002\/neu.10196.","journal-title":"J Neurobiol"},{"key":"6804_CR109","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1111\/pcmr.13008","volume":"35","author":"H Barek","year":"2022","unstructured":"Barek H, Zhao H, Heath K, Veraksa A, Sugumaran M. Drosophila yellow-h encodes dopaminechrome tautomerase: a new enzyme in the eumelanin biosynthetic pathway. Pigment Cell Melanoma Res. 2022;35:26\u201337. https:\/\/doi.org\/10.1111\/pcmr.13008.","journal-title":"Pigment Cell Melanoma Res"},{"key":"6804_CR110","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1007\/978-3-642-17815-3_8","volume-title":"Recent Advances in entomological research","author":"Q Song","year":"2011","unstructured":"Song Q, An S. Bursicon, a neuropeptide hormone that controls cuticle tanning and beyond. In: Liu T, Kang L, editors. Recent Advances in entomological research. Berlin Heidelberg: Springer Berlin, Heidelberg; 2011. p. 132\u201349."},{"key":"6804_CR111","doi-asserted-by":"publisher","first-page":"3597","DOI":"10.1002\/j.1460-2075.1986.tb04688.x","volume":"5","author":"W Chia","year":"1986","unstructured":"Chia W, Howes G, Martin M, Meng YB, Moses K, Tsubota S. Molecular analysis of the yellow locus of Drosophila. EMBO J. 1986;5:3597\u2013605. https:\/\/doi.org\/10.1002\/j.1460-2075.1986.tb04688.x.","journal-title":"EMBO J"},{"key":"6804_CR112","doi-asserted-by":"publisher","first-page":"41160","DOI":"10.1074\/jbc.M304303200","volume":"278","author":"A Richardt","year":"2003","unstructured":"Richardt A, Kemme T, Wagner S, Schwarzer D, Marahiel MA, Hovemann BT. Ebony, a novel nonribosomal peptide synthetase for \u03b2-alanine conjugation with biogenic amines in Drosophila. J Biol Chem. 2003;278:41160\u20136.","journal-title":"J Biol Chem"},{"key":"6804_CR113","doi-asserted-by":"publisher","first-page":"1272143","DOI":"10.3389\/fimmu.2023.1272143","volume":"14","author":"SA Khan","year":"2023","unstructured":"Khan SA, Kojour MAM, Han YS. Recent trends in insect gut immunity. Front Immunol. 2023;14:1272143.","journal-title":"Front Immunol"},{"key":"6804_CR114","doi-asserted-by":"publisher","first-page":"511","DOI":"10.1093\/chemse\/bjz046","volume":"44","author":"C Kennel","year":"2019","unstructured":"Kennel C, Gould EA, Larson ED, Salcedo E, Vickery T, Restrepo D, et al. Differential expression of mucins in murine olfactory versus respiratory epithelium. Chem Sens. 2019;44:511\u201321.","journal-title":"Chem Sens"},{"key":"6804_CR115","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1080\/19336934.2016.1162361","volume":"9","author":"H Sano","year":"2015","unstructured":"Sano H. Coupling of growth to nutritional status: the role of novel periphery-to-brain signaling by the CCHa2 peptide in Drosophila melanogaster. Fly. 2015;9:183\u20137. https:\/\/doi.org\/10.1080\/19336934.2016.1162361.","journal-title":"Fly"},{"key":"6804_CR116","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0133017","volume":"10","author":"GR Ren","year":"2015","unstructured":"Ren GR, Hauser F, Rewitz KF, Kondo S, Engelbrecht AF, Didriksen AK, et al. CCHamide-2 is an orexigenic brain-gut peptide in Drosophila. PLoS ONE. 2015;10:e0133017. https:\/\/doi.org\/10.1371\/journal.pone.0133017.","journal-title":"PLoS ONE"},{"key":"6804_CR117","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007279","volume":"14","author":"O Benguettat","year":"2018","unstructured":"Benguettat O, Jneid R, Soltys J, Loudhaief R, Brun-Barale A, Osman D, et al. The DH31\/CGRP enteroendocrine peptide triggers intestinal contractions favoring the elimination of opportunistic bacteria. PLoS Pathog. 2018;14:e1007279. https:\/\/doi.org\/10.1371\/journal.ppat.1007279.","journal-title":"PLoS Pathog"},{"key":"6804_CR118","doi-asserted-by":"publisher","DOI":"10.3389\/fevo.2022.948043\/full","volume":"10","author":"X Zhang","year":"2022","unstructured":"Zhang X, Shi Z, Yang C, Li J, Liu J, Zhang A. Gut transcriptome analysis of P450 genes and cytochrome P450 reductase in three moth species feeding on gymnosperms or angiosperms. Front Ecol Evol. 2022;10:948043. https:\/\/doi.org\/10.3389\/fevo.2022.948043\/full.","journal-title":"Front Ecol Evol"},{"key":"6804_CR119","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023180","volume":"6","author":"S Lahiri","year":"2011","unstructured":"Lahiri S, Shen K, Klein M, Tang A, Kane E, Gershow M, et al. Two alternating motor programs drive navigation in Drosophila Larva. PLoS ONE. 2011;6:e23180. https:\/\/doi.org\/10.1371\/journal.pone.0023180.","journal-title":"PLoS ONE"},{"key":"6804_CR120","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.4318","volume":"6","author":"J-F Ferveur","year":"2018","unstructured":"Ferveur J-F, Cortot J, Rihani K, Cobb M, Everaerts C. Desiccation resistance: effect of cuticular hydrocarbons and water content in Drosophila melanogaster adults. PeerJ. 2018;6:e4318.","journal-title":"PeerJ"},{"key":"6804_CR121","doi-asserted-by":"publisher","first-page":"2538","DOI":"10.1038\/s41598-017-02763-4","volume":"7","author":"Y Yan","year":"2017","unstructured":"Yan Y, Linger RJ, Scott MJ. Building early-larval sexing systems for genetic control of the Australian sheep blow fly Lucilia cuprina using two constitutive promoters. Sci Rep. 2017;7:2538.","journal-title":"Sci Rep"},{"key":"6804_CR122","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1007\/s00438-019-01622-3","volume":"295","author":"Y Yan","year":"2020","unstructured":"Yan Y, Williamson ME, Davis RJ, Andere AA, Picard CJ, Scott MJ. Improved transgenic sexing strains for genetic control of the Australian sheep blow fly Lucilia cuprina using embryo-specific gene promoters. Mol Genet Genom. 2020;295:287\u201398. https:\/\/doi.org\/10.1007\/s00438-019-01622-3.","journal-title":"Mol Genet Genom"},{"key":"6804_CR123","first-page":"179","volume-title":"Int Rev Cytol","author":"DJ Van der Horst","year":"2001","unstructured":"Van der Horst DJ, Van Marrewijk WJA, Diederen JHB. Adipokinetic hormones of insect: release, signal transduction, and responses. In: Int Rev Cytol. Cambridge: Academic Press; 2001. p. 179\u2013240."},{"key":"6804_CR124","doi-asserted-by":"publisher","first-page":"251","DOI":"10.3390\/ph7030251","volume":"7","author":"V \u010ce\u0159ovsk\u00fd","year":"2014","unstructured":"\u010ce\u0159ovsk\u00fd V, B\u00e9m R. Lucifensins, the Insect defensins of biomedical importance: the story behind maggot therapy. Pharmaceuticals. 2014;7:251\u201364.","journal-title":"Pharmaceuticals"},{"key":"6804_CR125","doi-asserted-by":"publisher","first-page":"2568","DOI":"10.1093\/emboj\/21.11.2568","volume":"21","author":"E De Gregorio","year":"2002","unstructured":"De Gregorio E. The toll and Imd pathways are the major regulators of the immune response in Drosophila. EMBO J. 2002;21:2568\u201379. https:\/\/doi.org\/10.1093\/emboj\/21.11.2568.","journal-title":"EMBO J"},{"key":"6804_CR126","doi-asserted-by":"publisher","first-page":"464","DOI":"10.1016\/j.celrep.2017.06.043","volume":"20","author":"C Martelli","year":"2017","unstructured":"Martelli C, Pech U, Kobbenbring S, Pauls D, Bahl B, Sommer MV, et al. SIFamide translates hunger signals into appetitive and feeding behavior in Drosophila. Cell Rep. 2017;20:464\u201378.","journal-title":"Cell Rep"},{"key":"6804_CR127","doi-asserted-by":"publisher","first-page":"50781","DOI":"10.1074\/jbc.M407842200","volume":"279","author":"K-S Lee","year":"2004","unstructured":"Lee K-S, You K-H, Choo J-K, Han Y-M, Yu K. Drosophila short neuropeptide F regulates food intake and body size. J Biol Chem. 2004;279:50781\u20139.","journal-title":"J Biol Chem"},{"key":"6804_CR128","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1631\/jzus.B2300355","volume":"25","author":"M Cholewi\u0144ski","year":"2024","unstructured":"Cholewi\u0144ski M, Chowa\u0144ski S, Lubawy J, Urba\u0144ski A, Walkowiak-Nowicka K, Marciniak P. Short neuropeptide F in integrated insect physiology. J Zhejiang Univ-Sci B. 2024;25:389\u2013409. https:\/\/doi.org\/10.1631\/jzus.B2300355.","journal-title":"J Zhejiang Univ-Sci B"},{"key":"6804_CR129","doi-asserted-by":"publisher","first-page":"1275","DOI":"10.1016\/j.neuron.2022.03.036","volume":"110","author":"L Deng","year":"2022","unstructured":"Deng L, Chiu IM. A neuropeptide regulates immunity across species. Neuron. 2022;110:1275\u20137.","journal-title":"Neuron"},{"key":"6804_CR130","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/B0-44-451924-6\/00050-8","volume-title":"Comprehensive molecular insect science","author":"JH Willis","year":"2005","unstructured":"Willis JH, Iconomidou VA, Smith RF, Hamodrakas SJ. Cuticular proteins. In: Gilbert LI, editor. Comprehensive molecular insect science. Amsterdam: Elsevier; 2005. p. 79\u2013109."},{"key":"6804_CR131","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/0020-1790(74)90036-5","volume":"4","author":"SH Kang","year":"1974","unstructured":"Kang SH, Fuchs MS. Isolation of a chymotrypsin inhibitor from Drosophila melanogaster. Insect Biochem. 1974;4:1\u20138.","journal-title":"Insect Biochem"}],"container-title":["Parasites &amp; Vectors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-025-06804-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13071-025-06804-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13071-025-06804-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,7]],"date-time":"2025-09-07T04:28:49Z","timestamp":1757219329000},"score":1,"resource":{"primary":{"URL":"https:\/\/parasitesandvectors.biomedcentral.com\/articles\/10.1186\/s13071-025-06804-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,9]]},"references-count":131,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["6804"],"URL":"https:\/\/doi.org\/10.1186\/s13071-025-06804-0","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.12.20.629795","asserted-by":"object"}]},"ISSN":["1756-3305"],"issn-type":[{"value":"1756-3305","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,9]]},"assertion":[{"value":"9 January 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 April 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"270"}}