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Vector-borne pathogens were screened by light microscopic examination of blood smears, point-of-care serology, and quantitative real-time polymerase chain reaction (qPCR). Haematology and serum biochemistry analyses were also performed.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Collectively, the SNAP<jats:sup>\u00ae<\/jats:sup> 4Dx<jats:sup>\u00ae<\/jats:sup> Plus Test provided 64% seropositive results, comprising <jats:italic>Ehrlichia<\/jats:italic> species (59%), <jats:italic>Anaplasma<\/jats:italic> species (45%), <jats:italic>Dirofilaria immitis<\/jats:italic> (2%), and <jats:italic>Borrelia burgdorferi<\/jats:italic> (&lt;\u20091%). Altogether, prevalence as determined by probe-based qPCR assays was 54%, comprising <jats:italic>Ehrlichia canis<\/jats:italic> (27%), <jats:italic>Hepatozoon canis<\/jats:italic> (25%), <jats:italic>Anaplasma<\/jats:italic> species (13%), and <jats:italic>Babesia vogeli<\/jats:italic> (8%). Light microscopy yielded the least number of positives, indicating a collective positive result of only 11% in screening for <jats:italic>Ehrlichia<\/jats:italic>, <jats:italic>Anaplasma<\/jats:italic>, <jats:italic>Hepatozoon<\/jats:italic>, <jats:italic>Babesia<\/jats:italic>, and microfilaria species. On the whole, Kunene and Otjozondjupa regions showed the highest pathogen prevalence (75%), and the lowest was from Erongo region (38%), on qPCR testing. Significant associations between tick presence and infection by <jats:italic>E. canis<\/jats:italic> (<jats:italic>P<\/jats:italic>\u2009=\u20090.001), <jats:italic>Anaplasma<\/jats:italic> species (<jats:italic>P<\/jats:italic>\u2009=\u20090.006), and <jats:italic>B. vogeli<\/jats:italic> (<jats:italic>P<\/jats:italic>\u2009=\u20090.008) were demonstrated. Likewise, relevant associations between haemoparasite infection and variables of patient signalment, history, and various disease manifestations were shown. Finally, significant associations were found between pathogen infection and numerous clinical pathology abnormalities of the erythron, leukon, and thrombon, including thrombocytopenia (<jats:italic>P<\/jats:italic>\u2009=\u20090.022).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Diagnostic modalities should be used contextually to test for canine pathogens, with due consideration of the limitations. Appropriate diagnostic testing such as qPCR, guided by relevant known associations with disease manifestation, should guide responsible treatment strategies and identify potential zoonotic risks in pets.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Graphical Abstract<\/jats:title>\n          <\/jats:sec>","DOI":"10.1186\/s13071-025-06906-9","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T08:52:44Z","timestamp":1752137564000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Prevalence, diagnostic evaluation, and disease associations of vector-borne pathogens in domestic dogs across Namibia: a multi-modal approach"],"prefix":"10.1186","volume":"18","author":[{"given":"Lourens","family":"de Villiers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan P.","family":"Schoeman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barend L.","family":"Penzhorn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umberto","family":"Molini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"de Villiers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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