{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T04:22:23Z","timestamp":1767068543204,"version":"3.48.0"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,12,30]],"date-time":"2025-12-30T00:00:00Z","timestamp":1767052800000},"content-version":"vor","delay-in-days":33,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Parasites Vectors"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The cestode\n                      <jats:italic>Dipylidium caninum<\/jats:italic>\n                      is known to infect dogs via the ingestion of an intermediate flea host, typically\n                      <jats:italic>Ctenocephalides felis<\/jats:italic>\n                      . Simparica Trio\n                      <jats:sup>\u00ae<\/jats:sup>\n                      is an oral combination drug product for dogs effective in the treatment and prevention of fleas, including\n                      <jats:italic>C.\u00a0felis<\/jats:italic>\n                      . Here, we report two laboratory studies evaluating the efficacy of a single administration of Simparica Trio at the minimum label dosage of 1.2\u00a0mg\/kg sarolaner, 24\u00a0\u00b5g\/kg moxidectin, and 5\u00a0mg\/kg pyrantel (as pamoate salt) in preventing\n                      <jats:italic>D.\u00a0caninum<\/jats:italic>\n                      infection in dogs for 1\u00a0month through killing of\n                      <jats:italic>C.\u00a0felis<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A total of 20 dogs (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200910 per group) proven to be suitable hosts for\n                      <jats:italic>C.\u00a0felis<\/jats:italic>\n                      were used in each of the two studies. Treatment occurred on day 0, with each dog given either the placebo or Simparica Trio. On days 0 (after treatment), 7, 14, 21, and 30, dogs were infested using 200 (\u00b1\u20095) unfed\n                      <jats:italic>D.\u00a0caninum<\/jats:italic>\n                      -infected\n                      <jats:italic>C.\u00a0felis<\/jats:italic>\n                      . Live flea counts were conducted on day 33 (72\u2009\u00b1\u20092\u00a0h after day 30 infestation). All dogs were euthanized on day 58, and each dog was necropsied for the recovery of\n                      <jats:italic>D.\u00a0caninum<\/jats:italic>\n                      scolexes from the gastrointestinal tract.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Placebo-treated dogs had adequate flea infestations and cestode infections in both studies. Simparica-Trio-treated dogs were free of fleas on day 33 (100% efficacy) and had significantly lower mean flea counts compared with placebo-treated dogs (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009\u2264\u20090.0007). Scolex counts in Simparica-Trio-treated dogs were also significantly decreased compared with placebo-treated dogs in both studies. The efficacy of Simparica Trio against\n                      <jats:italic>D.\u00a0caninum<\/jats:italic>\n                      based on least squares mean scolex counts was 100% (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.0001) in study 1 and 92.1% (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.0033) in study 2.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The efficacy provided by Simparica Trio against\n                      <jats:italic>C.\u00a0felis<\/jats:italic>\n                      at the minimum dosage of 1.2\u00a0mg\/kg sarolaner, 24\u00a0\u00b5g\/kg moxidectin, and 5\u00a0mg\/kg pyrantel (as pamoate salt) prevented\n                      <jats:italic>D.\u00a0caninum<\/jats:italic>\n                      infection in dogs for 1\u00a0month.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Graphical Abstract<\/jats:title>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-025-07129-8","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T19:07:25Z","timestamp":1764270445000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Simparica Trio\u00ae kills Ctenocephalides felis on dogs and provides month-long protection against the transmission of Dipylidium caninum"],"prefix":"10.1186","volume":"18","author":[{"given":"Lindsay","family":"Weaver","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alta","family":"Viljoen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riaan","family":"Maree","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lina","family":"D\u2019Hanis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelby","family":"Jones","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane","family":"Tonso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinier","family":"Zwiegers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julian","family":"Liebenberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sean","family":"Mahabir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keith","family":"Baker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Rodriguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris","family":"Adolph","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Geurden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,11,27]]},"reference":[{"key":"7129_CR1","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1186\/1756-3305-4-139","volume":"4","author":"G Dobler","year":"2011","unstructured":"Dobler G, Pfeffer M. 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R.Z. was paid external consultant to Zoetis. The authors declare that this study received funding from Zoetis. The funder had the following involvement in the study: decision to submit it for publication.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"515"}}