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Lyme disease, while commonly asymptomatic, can induce fever and intermittent lameness in dogs. Highly effective acaricidal products with a rapid onset of action along with prompt removal of attached ticks are important aspects of successful Lyme disease prevention strategies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Two studies were conducted with a total of 30 dogs each. Dogs were randomized to receive a control sham dose, Credelio Quattro, or Credelio. Treatment was administered on Day 0 in a fed state. On Day 28, all dogs were experimentally infested with wild-caught adult\n                      <jats:italic>Ixodes scapularis<\/jats:italic>\n                      . Blood samples for\n                      <jats:italic>B. burgdorferi<\/jats:italic>\n                      antibody analysis utilizing the SNAP 4Dx Plus and Lyme Quant C\n                      <jats:sub>6<\/jats:sub>\n                      tests were collected on Days 27, 49, 63, 77, 91, and 105. Skin biopsies were collected from four different areas of heavy tick attachment from each dog  for polymerase chain reaction (PCR) detection of\n                      <jats:italic>B. burgdorferi<\/jats:italic>\n                      on Day 104 or 105.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      All control dogs demonstrated adequate\n                      <jats:italic>I. scapularis<\/jats:italic>\n                      infestation rates on Day 33 in both studies. In Study 2, on Day 27, one control dog tested positive for\n                      <jats:italic>B. burgdorferi<\/jats:italic>\n                      on the Lyme Quant C\n                      <jats:sub>6<\/jats:sub>\n                      test, prior to experimental tick infestation, and therefore was excluded from analysis. A total of eight out of 10 (Study 1) and nine out of nine (Study 2) control dogs tested positive for\n                      <jats:italic>B. burgdorferi<\/jats:italic>\n                      on at least one test after Day 27. One dog in the Credelio Quattro-treated group tested positive for\n                      <jats:italic>B. burgdorferi<\/jats:italic>\n                      on SNAP 4Dx Plus on Day 105 in Study 1 but was negative on all other tests and study days. None of the dogs treated with Credelio tested positive for\n                      <jats:italic>B. burgdorferi<\/jats:italic>\n                      at any point during either study.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The laboratory studies described herein confirm that a single dose of lotilaner, at the minimum effective dosage of 20\u00a0mg\/kg, administered as Credelio Quattro, in combination with moxidectin, praziquantel, and pyrantel or Credelio, is effective for the prevention of transmission of\n                      <jats:italic>B. burgdorferi<\/jats:italic>\n                      from infected\n                      <jats:italic>I. scapularis<\/jats:italic>\n                      for a full month in dogs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Graphical Abstract<\/jats:title>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-025-07136-9","type":"journal-article","created":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T08:55:09Z","timestamp":1765011309000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Efficacy of Credelio Quattro\u2122 (lotilaner, moxidectin, praziquantel, and pyrantel chewable tablets) and Credelio\u2122 (lotilaner) chewable tablets in the prevention of Borrelia burgdorferi transmission from infected Ixodes scapularis in dogs"],"prefix":"10.1186","volume":"19","author":[{"given":"William","family":"Anderson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly D.","family":"Savadelis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott","family":"Wiseman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelmoneim","family":"Mansour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riaan","family":"Maree","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Young","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,6]]},"reference":[{"key":"7136_CR1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9122412","author":"S Hussain","year":"2021","unstructured":"Hussain S, Hussain A, Aziz U, Song B, Zeb J, George D, et al. 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AM and RM were contracted to conduct the studies.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"23"}}