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Our primary objective was to determine whether\n                      <jats:italic>T. chanchalensis<\/jats:italic>\n                      was present in Alaska, using a new molecular method that enables the description of the prevalence, co-infection, host associations, and risk factors for\n                      <jats:italic>Trichinella<\/jats:italic>\n                      spp. infection in wild carnivores.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Trichinella<\/jats:italic>\n                      spp. larvae were recovered through artificial digestion of muscle and genotyped using next-generation sequencing (NGS).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Trichinella<\/jats:italic>\n                      spp. larvae were detected in 53\/157 (34%) animals, namely wolverines (\n                      <jats:italic>Gulo gulo<\/jats:italic>\n                      ), arctic foxes (\n                      <jats:italic>Vulpes lagopus<\/jats:italic>\n                      ), red foxes (\n                      <jats:italic>Vulpes vulpes<\/jats:italic>\n                      ), coyotes (\n                      <jats:italic>Canis latrans<\/jats:italic>\n                      ), wolves (\n                      <jats:italic>Canis lupus<\/jats:italic>\n                      ), brown bears (\n                      <jats:italic>Ursus arctos<\/jats:italic>\n                      ), and polar bears (\n                      <jats:italic>Ursus maritimus<\/jats:italic>\n                      ), but not in black bears (\n                      <jats:italic>Ursus americanus<\/jats:italic>\n                      ) or lynx (\n                      <jats:italic>Lynx canadensis<\/jats:italic>\n                      ). Prevalence was highest in polar bears and wolverines, while intensity (larvae per gram, LPG) was highest in red foxes, arctic foxes, and wolves. Most animals (65%) harbored single infections with\n                      <jats:italic>Trichinella nativa<\/jats:italic>\n                      , followed by mixed infections of\n                      <jats:italic>T. nativa<\/jats:italic>\n                      and\n                      <jats:italic>Trichinella<\/jats:italic>\n                      T6 (33%). A single wolverine was infected with\n                      <jats:italic>T. nativa<\/jats:italic>\n                      , T6, and\n                      <jats:italic>T. chanchalensis<\/jats:italic>\n                      . Combining NGS with statistical methods, we found no evidence of competition between\n                      <jats:italic>T. nativa<\/jats:italic>\n                      and T6 in host muscles.\n                      <jats:italic>Trichinella<\/jats:italic>\n                      spp. infection (primarily\n                      <jats:italic>T. nativa<\/jats:italic>\n                      ) was the highest in the Northwestern region, whereas T6 infection probability was higher in the Interior and Southern regions, suggesting differences in environmental resistance even among these three taxa. In a single, highly infected brown bear, we detected a rare case of\n                      <jats:italic>Trichinella spiralis<\/jats:italic>\n                      of foreign origin based on whole-genome sequencing, suggesting illegal importation and disposal of meat.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      We report a new geographical record for\n                      <jats:italic>T. chanchalensis<\/jats:italic>\n                      and a rare finding of\n                      <jats:italic>T. spiralis<\/jats:italic>\n                      in North American wildlife, and demonstrate the utility of new NGS methods for describing the ecology of parasites maintained in wildlife hosts commonly presenting as co-infections.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Graphical Abstract<\/jats:title>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-025-07142-x","type":"journal-article","created":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T09:08:30Z","timestamp":1765357710000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["First report of Trichinella chanchalensis, and detection of foreign Trichinella spiralis, in wildlife in Alaska"],"prefix":"10.1186","volume":"19","author":[{"given":"Cody J.","family":"Malone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberlee","family":"Beckmen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphaela","family":"Stimmelmayr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladislav A.","family":"Lobanov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maarten J.","family":"Voordouw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayne","family":"Ellis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily J.","family":"Jenkins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,10]]},"reference":[{"key":"7142_CR1","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1016\/j.fawpar.2016.07.001","volume":"4","author":"E Pozio","year":"2016","unstructured":"Pozio E. 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