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While hookworm infections in pets are frequently reported in Europe primarily through coproscopic studies, there are limited data regarding their presence in wild carnivores. To address this, this study aimed to assess the diversity, prevalence, and distribution of hookworms in wild canids and felids from Romania through both morphological and molecular analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      From November 2011 to February 2025, 319 carcasses belonging to six species of wild canids and felids from Romania [23\u00a0gray wolves (\n                      <jats:italic>Canis lupus<\/jats:italic>\n                      ), 137 golden jackals (\n                      <jats:italic>Canis aureus<\/jats:italic>\n                      ), 79 red foxes (\n                      <jats:italic>Vulpes vulpes<\/jats:italic>\n                      ), 2 raccoon dogs (\n                      <jats:italic>Nyctereutes procyonoides)<\/jats:italic>\n                      , 70 European wildcats (\n                      <jats:italic>Felis silvestris<\/jats:italic>\n                      ), and 8 Eurasian lynxes (\n                      <jats:italic>Lynx lynx<\/jats:italic>\n                      )] were collected as road kills or legally hunted. Hookworms were recovered from the intestinal tract during necropsy and preserved in formalin for morphological examination and in absolute ethanol for genetic analysis. Genomic DNA was extracted and analyzed using a PCR targeting a barcode region of the second nuclear ribosomal internal transcribed spacer (ITS-2), followed by sequencing. Sequencing results were compared with other entries from GenBank\u2122.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The overall hookworm infection rate was 14.1%, with hookworms detected in 4 wolves (17.4%), 23 golden jackals (16.8%), 11 European wildcats (15.7%), 4 red foxes (5.1%), 2 raccoon dogs (100%), and 1 lynx (12.5%). Three hookworm species were identified:\n                      <jats:italic>Uncinaria stenocephala<\/jats:italic>\n                      ,\n                      <jats:italic>Ancylostoma caninum<\/jats:italic>\n                      , and\n                      <jats:italic>A. tubaeforme<\/jats:italic>\n                      . Molecular analysis revealed 14 unique sequences, comprising nine haplotypes of\n                      <jats:italic>U. stenocephala<\/jats:italic>\n                      , three of\n                      <jats:italic>A. caninum<\/jats:italic>\n                      , and two of\n                      <jats:italic>A. tubaeforme<\/jats:italic>\n                      . We report for the first time the Eurasian lynx as a host for\n                      <jats:italic>A. caninum<\/jats:italic>\n                      , expanding the known host range of this species.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study provides the first comprehensive molecular assessment of hookworm diversity in European wild carnivores, showing new host\u2013parasite associations and highlighting the importance of these hosts as reservoirs for domestic pets and, potentially, humans. The detected haplotypes showed high similarity to isolates from Europe, Asia, and the Americas, indicating a broad global connectivity of hookworm populations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Graphical abstract<\/jats:title>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-025-07219-7","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T15:45:26Z","timestamp":1770219926000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ancylostomatidae in wild canids and felids from Romania: new host associations and haplotype diversity"],"prefix":"10.1186","volume":"19","author":[{"given":"Ioana Bianca","family":"Mitrea","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreea Daniela","family":"Iani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u0103lin Mircea","family":"Gherman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina Daniela","family":"Cazan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela Monica","family":"Ionic\u0103","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0218tefan Ovidiu","family":"Rabei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgiana","family":"Deak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mihai Sorin","family":"Cernea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vasile","family":"Alexe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel Bogdan","family":"Chi\u0219amera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mihai","family":"Marinov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrei Daniel","family":"Mihalca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,2,4]]},"reference":[{"key":"7219_CR1","doi-asserted-by":"publisher","first-page":"1546195","DOI":"10.3389\/fpara.2025.1546195","volume":"4","author":"R Kaminsky","year":"2025","unstructured":"Kaminsky R, M\u00e4ser P. 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