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In the absence of effective alternatives to chemical insecticides for eliminating adult mosquitoes, healthcare facilities may become focal points for arbovirus transmission, compromising the safety of patients and healthcare employees. Vector control remains the main approach for mitigating arbovirus transmission. This study investigated the impact of an effective integrated control program (ICP) implemented in a hospital in Recife, Pernambuco, Brazil, where arbovirus circulation in mosquitoes was detected.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Entomological monitoring was conducted through mechanical aspiration inside the Hospital das Cl\u00ednicas (HC). Specimens were counted, identified, grouped by species, classified according to blood-feeding status, and recorded by capture station. Viral infection was assessed using nonstructural protein 1 (NS1) by enzyme-linked immunosorbent assay (ELISA) and a triplex reverse transcription quantitative polymerase chain reaction (RT-qPCR) for the detection of DENV, ZIKV, and CHIKV. Infection rates were calculated using the minimum infection rate (MIR). ICP actions included environmental management, larvicide application at breeding sites, ovitraps to collect and destroy eggs, toxic sugar baits, and intensified mechanical aspiration to eliminate adult mosquitoes. Three phases were defined: pre-ICP (initial survey), ICP (all actions), and post-ICP (larvicidal treatment).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      During the ICP, a sustained reduction in female mosquito density was observed, reaching 99% for\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      and 88% for\n                      <jats:italic>Culex quinquefasciatus<\/jats:italic>\n                      . Six months after ICP, ELISA detected infected females in 9 of 14 stations (64%). The MIR for\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      decreased from 50 to 44, while\n                      <jats:italic>Cx. quinquefasciatus<\/jats:italic>\n                      showed a slight increase. RT-qPCR revealed 11 ZIKV-positive pools (eight\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      and three\n                      <jats:italic>Cx. quinquefasciatus<\/jats:italic>\n                      ). After 12\u00a0months of ICP, only one\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      DENV-positive pool was detected by both techniques in a single station (pediatrics). Both techniques detected infection even in pools containing a single female, underscoring their high sensitivity. At 18\u00a0months of ICP, all pools tested negative for arboviruses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Molecular and antigen-based approaches confirmed the mitigation of arbovirus circulation in hospital-associated mosquitoes, demonstrating that the continuous elimination of\n                      <jats:italic>Ae. aegypti<\/jats:italic>\n                      females (&gt;\u200999%) is critical in these settings to prevent nosocomial transmission.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Graphical abstract<\/jats:title>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-025-07241-9","type":"journal-article","created":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T10:45:24Z","timestamp":1773398724000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Control and mitigation of dengue and Zika virus transmission in a hospital in Recife, Brazil: a successful experience with an integrated control program against Aedes aegypti"],"prefix":"10.1186","volume":"19","author":[{"given":"Helena Emanuela","family":"Candida-Silva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrique Rafael Pontes","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaziela de Arruda","family":"Mendon\u00e7a","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael Alves","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larissa","family":"Krokovsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Let\u00edcia de Oliveira","family":"Martins","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathyanne Ellen da Silva","family":"Barbosa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederico Jorge","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e1udia Maria Fontes","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Const\u00e2ncia Fl\u00e1via Junqueira","family":"Ayres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo Henrique Santos","family":"Paiva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Alice Varjal","family":"de Melo-Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,3,13]]},"reference":[{"key":"7241_CR1","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1590\/0104-1169.2925.2395","volume":"22","author":"APM Pontes","year":"2014","unstructured":"Pontes APM, Oliveira DC, Gomes AMT. 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