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No systematic pathogen survey has previously been conducted on host-seeking individuals of this species. This study aimed to provide the first comprehensive molecular investigation of bacterial, protozoan, and viral tick-borne pathogens (TBPs) in questing\n                      <jats:italic>H. punctata<\/jats:italic>\n                      populations in Central and Northeastern Anatolia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A total of 96 host-seeking adult\n                      <jats:italic>H. punctata<\/jats:italic>\n                      were collected from 29 sampling sites in 11 districts across seven provinces. DNA and RNA extracts were screened using a multi-agent polymerase chain reaction (PCR)\u2212-sequencing panel targeting a broad range of TBPs. Positive amplicons were sequenced for species identification, and complete genome sequencing was performed for the detected Burana virus strain. Phylogenetic analyses were conducted using maximum-likelihood and Bayesian approaches.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Microorganisms detected included\n                      <jats:italic>Babesia major<\/jats:italic>\n                      (5.21%),\n                      <jats:italic>Theileria orientalis<\/jats:italic>\n                      (1.04%), an uncharacterized\n                      <jats:italic>Ehrlichia<\/jats:italic>\n                      sp. (1.04%), and spotted fever group rickettsiae (3.13%) comprising\n                      <jats:italic>Candidatus<\/jats:italic>\n                      Rickettsia yenbekshikazakhensis and\n                      <jats:italic>Rickettsia hoogstraalii<\/jats:italic>\n                      .\n                      <jats:italic>Coxiella burnetii<\/jats:italic>\n                      was identified at the highest prevalence (20.83%), representing the first detection of this agent in questing\n                      <jats:italic>H. punctata<\/jats:italic>\n                      in T\u00fcrkiye. Several ticks carried mixed infections. Notably, Burana virus was detected in one specimen\u2014marking the first confirmed occurrence of this orthonairovirus outside Central Asia. Complete S, M, and L segment genomes were recovered, and phylogenetic analyses showed a close\u2014though not identical\u2014relationship to strains from Kyrgyzstan and Xinjiang.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study provides the first systematic assessment of TBPs in host-seeking\n                      <jats:italic>H. punctata<\/jats:italic>\n                      in T\u00fcrkiye and documents, for the first time in this species, the presence of Burana virus,\n                      <jats:italic>Candidatus<\/jats:italic>\n                      R. yenbekshikazakhensis,\n                      <jats:italic>T. orientalis<\/jats:italic>\n                      , and\n                      <jats:italic>C. burnetii<\/jats:italic>\n                      . The findings highlight\n                      <jats:italic>H. punctata<\/jats:italic>\n                      as an underrecognized but epidemiologically relevant tick species in Anatolia and reveal previously undocumented microorganism circulation, with important implications for surveillance of emerging viral and zoonotic threats.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Graphical Abstract<\/jats:title>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-026-07367-4","type":"journal-article","created":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T15:46:48Z","timestamp":1774626408000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular screening of tick-borne pathogens in host-seeking Haemaphysalis punctata Canestrini &amp; Fanzago, 1878 (Ixodoidea: Ixodidae) in Anatolia with the first report of Burana virus"],"prefix":"10.1186","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4478-2521","authenticated-orcid":false,"given":"\u00d6mer","family":"Orkun","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tu\u011fba","family":"\u00d6zdemir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maide Nur","family":"G\u00fcndo\u011fdu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mesut","family":"Yi\u011fit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmet","family":"Deniz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zati","family":"Vatansever","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2026,3,27]]},"reference":[{"key":"7367_CR1","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1007\/BF00259499","volume":"37","author":"J Nosek","year":"1971","unstructured":"Nosek J. 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