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Prevention of pathogen transmission can be achieved by regular tick control and prophylaxis. The present study evaluated the efficacy of a single oral application of lotilaner (Credelio\n                      <jats:sup>\u2122<\/jats:sup>\n                      , Elanco) in dogs for blocking pathogen transmission compared to a negative control group.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Twenty-four\n                      <jats:italic>B.\u00a0canis<\/jats:italic>\n                      -negative dogs were allocated to three groups (\n                      <jats:italic>n\u2009<\/jats:italic>\n                      =\u20098 each). The two treatment groups were treated once orally with Credelio either on Day 0 or 23, while the negative control was sham-dosed accordingly. All dogs were challenged with\n                      <jats:italic>B. canis<\/jats:italic>\n                      -infected\n                      <jats:italic>D. reticulatus<\/jats:italic>\n                      on Day 28, i.e., 28- or 5-days post-treatment (p.t.), for the lotilaner-treated groups. Tick efficacy was assessed by in situ tick counts 24 and 48\u00a0h after infestation (Days 29 and 30) and final tick counts and removals 120\u00a0h after infestation (Day 33).\n                    <\/jats:p>\n                    <jats:p>Clinical examinations, hematology (blood smear), serology (IFAT) and blood PCR were performed in all dogs systematically and in case of suspected clinical babesiosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A single oral treatment with Credelio 28 or 5 days prior to infestation with\n                      <jats:italic>B.\u00a0canis<\/jats:italic>\n                      -infected\n                      <jats:italic>D. reticulatus<\/jats:italic>\n                      resulted in 100% blocking efficacy against\n                      <jats:italic>B.\u00a0canis<\/jats:italic>\n                      transmission, as compared to untreated dogs (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). The lotilaner-treated dogs were healthy and did not show any clinical or hematological signs of\n                      <jats:italic>B.\u00a0canis<\/jats:italic>\n                      infection. No treated dog seroconverted or showed a positive PCR. The untreated negative controls tested positive for\n                      <jats:italic>B. canis<\/jats:italic>\n                      from Day 35 onward. All negative controls developed\n                      <jats:italic>B. canis<\/jats:italic>\n                      infection by Day 36 (clinical signs, blood smear, PCR). From Day 43 onward, positive IFAT was also observed.\n                    <\/jats:p>\n                    <jats:p>Lotilaner administered on Day 0 or Day 23 demonstrated tick efficacy of 99.7 and 100%, respectively (Day 33).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study confirmed a single oral application of lotilaner chewable tablets was highly effective (100%) against\n                      <jats:italic>D. reticulatus<\/jats:italic>\n                      , blocking the transmission of\n                      <jats:italic>B.\u00a0canis<\/jats:italic>\n                      to dogs at the beginning as well as at the end of the monthly treatment interval of Credelio.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13071-026-07606-8","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T14:04:51Z","timestamp":1787148291000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Efficacy of lotilaner (Credelio\u2122 chewable tablets) for preventing Babesia canis transmission to dogs by infected Dermacentor reticulatus ticks"],"prefix":"10.1186","volume":"19","author":[{"given":"Katrin","family":"Deuster","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura A.","family":"Webb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carin","family":"Rautenbach","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William E.","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinier","family":"Zwiegers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friederike","family":"Kr\u00e4mer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brad","family":"Hayes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Young","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"7606_CR1","doi-asserted-by":"publisher","first-page":"1788","DOI":"10.1016\/j.meegid.2012.07.004","volume":"12","author":"L Schnittger","year":"2012","unstructured":"Schnittger L, Rodriguez AE, Florin-Christensen M, Morrison DA. 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Katrin Deuster, Laura A. Webb, Lisa Young, William E. Anderson, and Brad Hayes are current employees of Elanco Animal Health. Carin Rautenbach was contracted as the study investigator for this study at the study site. Reinier Zwiegers was contracted as the study monitor for this study. Friederike Kr\u00e4mer is working in a project funded by Elanco Animal Health at TransMIT GmbH, Germany. Credelio\u2122 is a trademark of Elanco, Indianapolis, IN, USA or its affiliates.","order":3,"name":"Ethics","label":"Competing interest","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"379"}}