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The purpose of our study is to determine the role of ROR\u03b3t expression in T cells on the development of collagen-induced arthritis (CIA).<\/jats:p>\n                     <\/jats:sec><jats:sec>\n                        <jats:title>Methods<\/jats:title>\n                        <jats:p>CIA was induced in C57BL\/6 and T cell-specific ROR\u03b3t transgenic (ROR\u03b3t Tg) mice. At day 10 post-1st-immunization, lymph node (LN) cells were cultured with type II collagen (CII), and the expression levels of various cytokines and transcription factors on CD4<jats:sup>+<\/jats:sup> T cells were measured. Total cells or CD4<jats:sup>+<\/jats:sup> cells of draining LN were harvested from each mouse group after CII-immunization and transferred into C57BL\/6 mice, and then CIA was induced in recipient mice. The expression levels of ROR\u03b3t and other surface antigens, and the production of cytokines were analyzed in forkhead box P3 (Foxp3)<jats:sup>+<\/jats:sup> regulatory T (Treg) cells. Foxp3<jats:sup>+<\/jats:sup> Treg cells were analyzed for suppressive activity against proliferation of effector CD4<jats:sup>+<\/jats:sup> T cells. Interlukin (IL)-10 neutralizing antibody was administrated in the course of CIA.<\/jats:p>\n                     <\/jats:sec><jats:sec>\n                        <jats:title>Results<\/jats:title>\n                        <jats:p>CIA was significantly suppressed in ROR\u03b3t Tg mice compared with C57BL\/6 mice. ROR\u03b3t expression and IL-17 production were significantly higher in CII-reactive CD4<jats:sup>+<\/jats:sup> T cells from ROR\u03b3t Tg mice. Arthritis was significantly attenuated in C57BL\/6 mice recipient of cells from ROR\u03b3t Tg mice. Most of Foxp3<jats:sup>+<\/jats:sup> Treg cells expressed ROR\u03b3t, produced IL-10 but not IL-17, and overexpressed CC chemokine receptor 6 (CCR6) and surface antigens related to the suppressive activity of Foxp3<jats:sup>+<\/jats:sup> Treg cells in ROR\u03b3t Tg mice. In vitro suppression assay demonstrated significant augmentation of the suppressive capacity of Foxp3<jats:sup>+<\/jats:sup> Treg cells in ROR\u03b3t Tg mice. CIA was exacerbated in both C57BL\/6 mice and ROR\u03b3t Tg mice by the treatment of anti-IL-10 antibody.<\/jats:p>\n                     <\/jats:sec><jats:sec>\n                        <jats:title>Conclusion<\/jats:title>\n                        <jats:p>Our results indicated that ROR\u03b3t overexpression in T cells protected against the development of CIA. The protective effects were mediated, at least in part, through the anti-inflammatory effects including high production of IL-10 of ROR\u03b3t<jats:sup>+<\/jats:sup>Foxp3<jats:sup>+<\/jats:sup> Treg cells.<\/jats:p>\n                     <\/jats:sec>","DOI":"10.1186\/s13075-015-0606-5","type":"journal-article","created":{"date-parts":[[2015,4,17]],"date-time":"2015-04-17T17:51:40Z","timestamp":1429293100000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Involvement of ROR\u03b3t-overexpressing T cells in the development of autoimmune arthritis in mice"],"prefix":"10.1186","volume":"17","author":[{"given":"Yuya","family":"Kondo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaojin","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Tahara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mana","family":"Iizuka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Yokosawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shunta","family":"Kaneko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiji","family":"Segawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroto","family":"Tsuboi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keigyou","family":"Yoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoru","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isao","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Sumida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2015,4,20]]},"reference":[{"key":"606_CR1","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1111\/j.1600-065X.2010.00951.x","volume":"238","author":"J Zhu","year":"2010","unstructured":"Zhu J, Paul WE. 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