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However, it remains unknown whether Postn loss-of-function can delay or prevent the development of OA. In this study, we sought to\u00a0better understand the role of Postn in OA development and assessed the functional impact of <jats:italic>Postn<\/jats:italic> deficiency on post-traumatic and age-related OA in mice.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>The effects of <jats:italic>Postn<\/jats:italic> deficiency were studied in two murine experimental OA models using <jats:italic>Postn<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> (<jats:italic>n<\/jats:italic>\u00a0=\u200932) and littermate wild-type (<jats:italic>wt<\/jats:italic>) mice (<jats:italic>n<\/jats:italic>\u00a0=\u200936). Post-traumatic OA was induced by destabilization of the medial meniscus (DMM) in 10-week-old mice (<jats:italic>n<\/jats:italic>\u00a0=\u200920); age-related OA was analyzed in 24-month-old mice (<jats:italic>n<\/jats:italic>\u00a0=\u200913). Cartilage degeneration was assessed histologically using the OARSI scoring system, and synovitis was evaluated by measuring the synovial lining cell layer and the cells density in the synovial stroma. Bone changes were measured by \u03bcCT analysis. Serum levels of Postn were determined by ELISA. Expression of Postn and collagenase-3 (MMP-13) was measured by immunostaining. RNA-seq was performed on chondrocytes isolated from 21-day old <jats:italic>Postn<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> (<jats:italic>n<\/jats:italic>\u00a0=\u20093) and <jats:italic>wt<\/jats:italic> mice (<jats:italic>n<\/jats:italic>\u00a0=\u20093) to discover genes and pathways altered by <jats:italic>Postn<\/jats:italic> knockout.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p><jats:italic>Postn<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice exhibited significantly reduced cartilage degeneration and OARSI score relative to <jats:italic>wt<\/jats:italic> mice in post-traumatic OA after 8 weeks (maximum: 2.37\u2009\u00b1\u20090.74 vs. 4.00\u2009\u00b1\u20091.20, <jats:italic>P<\/jats:italic>\u00a0=\u20090.011; summed: 9.31\u2009\u00b1\u20092.52 vs. 21.44\u2009\u00b1\u20096.01, <jats:italic>P<\/jats:italic>\u00a0=\u20090.0002) and spontaneous OA (maximum: 1.93\u2009\u00b1\u20090.45 vs. 3.58\u2009\u00b1\u20091.16, <jats:italic>P<\/jats:italic>\u00a0=\u20090.014; summed: 6.14\u2009\u00b1\u20091.57 vs. 11.50\u2009\u00b1\u20093.02, <jats:italic>P<\/jats:italic>\u00a0=\u20090.003). Synovitis was significantly lower in <jats:italic>Postn<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice than <jats:italic>wt<\/jats:italic> only in the DMM model (1.88\u2009\u00b1\u20091.01 vs. 3.17\u2009\u00b1\u20090.63; <jats:italic>P<\/jats:italic>\u00a0=\u20090.039). <jats:italic>Postn<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice also showed lower trabecular bone parameters such as BV\/TV, vBMD, Tb.Th, and Tb.N and high Tb. Sp in both models. <jats:italic>Postn<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice had negligible levels of serum Postn compared with <jats:italic>wt<\/jats:italic>. Immunofluorescent studies of cartilage indicated that <jats:italic>Postn<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice expressed lower MMP-13 levels than <jats:italic>wt <\/jats:italic>mice. RNA-seq revealed that cell-cell-adhesion and cell-differentiation processes were enriched in <jats:italic>Postn<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> mice, while those related to cell-cycle and DNA-repair were enriched in <jats:italic>wt<\/jats:italic> mice.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p><jats:italic>Postn<\/jats:italic> deficiency protects against DMM-induced post-traumatic and age-related spontaneous OA. RNA-seq findings warrant further investigations to\u00a0better understand the mechanistic role of Postn and its potential as a therapeutic target in OA.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13075-021-02477-z","type":"journal-article","created":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T06:03:00Z","timestamp":1617861780000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Periostin loss-of-function protects mice from post-traumatic and age-related osteoarthritis"],"prefix":"10.1186","volume":"23","author":[{"given":"Mukundan","family":"Attur","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Cai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianzhen","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weili","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric D.","family":"Tycksen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Samuels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert H.","family":"Brophy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven B.","family":"Abramson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4826-4331","authenticated-orcid":false,"given":"Muhammad Farooq","family":"Rai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,4,8]]},"reference":[{"issue":"12","key":"2477_CR1","doi-asserted-by":"publisher","first-page":"3546","DOI":"10.1002\/art.24984","volume":"60","author":"H Kotlarz","year":"2009","unstructured":"Kotlarz H, Gunnarsson CL, Fang H, Rizzo JA. 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