{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T13:10:41Z","timestamp":1794316241916,"version":"4.9.0"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,6,21]],"date-time":"2025-06-21T00:00:00Z","timestamp":1750464000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2025,6,21]],"date-time":"2025-06-21T00:00:00Z","timestamp":1750464000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Diabetol Metab Syndr"],"DOI":"10.1186\/s13098-025-01823-4","type":"journal-article","created":{"date-parts":[[2025,6,21]],"date-time":"2025-06-21T03:03:00Z","timestamp":1750474980000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Seagrass Enhalus acoroides extract mitigates obesity and diabetes via GLP-1, PPAR\u03b3, SREBP-1c modulation and gut microbiome restoration in diabetic zebrafish"],"prefix":"10.1186","volume":"17","author":[{"given":"Marcello Mikhael","family":"Kadharusman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rony Abdi","family":"Syahputra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudy","family":"Kurniawan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin","family":"Hadinata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond Rubianto","family":"Tjandrawinata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurpudji Astuti","family":"Taslim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffaele","family":"Romano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonello","family":"Santini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fahrul","family":"Nurkolis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,6,21]]},"reference":[{"key":"1823_CR1","unstructured":"Dianna J, Magliano; Edward J, Boyko. Irini Genitsaridi; Lorenzo Piemonte; Phil Riley; Paraskevi Salpea Diabetes Atlas 11th Edition; 11th ed.; International Diabetes Federation: Brussels, 2025; ISBN 978-2-930229-96-6."},{"key":"1823_CR2","unstructured":"Tim Lobstein; Rachel Jackson-Leach. Jaynaide powis; Hannah brinsden; Maggie Gray World obesity atlas 2023; World Obesity Federation: London."},{"key":"1823_CR3","doi-asserted-by":"publisher","first-page":"10449","DOI":"10.3390\/ijms241310449","volume":"24","author":"M-S Popoviciu","year":"2023","unstructured":"Popoviciu M-S, P\u0103duraru L, Yahya G, Metwally K, Cavalu S. Emerging role of GLP-1 agonists in obesity: A comprehensive review of randomised controlled trials. Int J Mol Sci. 2023;24:10449. https:\/\/doi.org\/10.3390\/ijms241310449.","journal-title":"Int J Mol Sci"},{"key":"1823_CR4","doi-asserted-by":"publisher","first-page":"1197","DOI":"10.3390\/ijms19041197","volume":"19","author":"M Botta","year":"2018","unstructured":"Botta M, Audano M, Sahebkar A, Sirtori C, Mitro N, Ruscica MPPAR. Agonists and metabolic syndrome: an established role?? Int J Mol Sci. 2018;19:1197. https:\/\/doi.org\/10.3390\/ijms19041197.","journal-title":"Int J Mol Sci"},{"key":"1823_CR5","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1016\/j.tibs.2018.01.005","volume":"43","author":"RA DeBose-Boyd","year":"2018","unstructured":"DeBose-Boyd RA, Ye J. SREBPs in lipid metabolism, insulin signaling, and beyond. Trends Biochem Sci. 2018;43:358\u201368. https:\/\/doi.org\/10.1016\/j.tibs.2018.01.005.","journal-title":"Trends Biochem Sci"},{"key":"1823_CR6","doi-asserted-by":"publisher","unstructured":"Fatmawati Y, Sandrina S, Nur Aina R, Narulita E. Molecular Docking analysis of seagrass (Enhalus Acoroides) phytochemical compounds as an antidiabetic. J Biol Res - Boll Della Soc Ital Biol Sper. 2022;95. https:\/\/doi.org\/10.4081\/jbr.2022.10224.","DOI":"10.4081\/jbr.2022.10224"},{"key":"1823_CR7","doi-asserted-by":"publisher","first-page":"365","DOI":"10.3390\/md22080365","volume":"22","author":"RR Tjandrawinata","year":"2024","unstructured":"Tjandrawinata RR, Nurkolis FA, Comparative. Analysis on impact of extraction methods on carotenoids composition, antioxidants, antidiabetes, and antiobesity properties in seagrass Enhalus acoroides: in Silico and in vitro study. Mar Drugs. 2024;22:365. https:\/\/doi.org\/10.3390\/md22080365.","journal-title":"Mar Drugs"},{"key":"1823_CR8","doi-asserted-by":"publisher","first-page":"105921","DOI":"10.1016\/j.jff.2023.105921","volume":"112","author":"M-T Nguyen","year":"2024","unstructured":"Nguyen M-T, Bui T-B-H, Pham V-H, Tran M-D, Nguyen Q-V. Syzygium zeylanicum (L.) DC. Polyphenols exhibit Anti-Diabetic activity by modulation of ACC1, SGLT1, and GLP-1 genes and restoration of gut microbiota in overfeeding and high glucose Exposure-Induced diabetic zebrafish. J Funct Foods. 2024;112:105921. https:\/\/doi.org\/10.1016\/j.jff.2023.105921.","journal-title":"J Funct Foods"},{"key":"1823_CR9","doi-asserted-by":"publisher","first-page":"18449","DOI":"10.1038\/s41598-023-44462-3","volume":"13","author":"A Reshma","year":"2023","unstructured":"Reshma A, Tamilanban T, Chitra V, Subramaniyan V, Gupta G, Fuloria NK, Sekar M, Fuloria S, Sahu R, Narayanan J, et al. Anti-Obesity effects of olivetol in adult zebrafish model induced by Short-Term High-Fat diet. Sci Rep. 2023;13:18449. https:\/\/doi.org\/10.1038\/s41598-023-44462-3.","journal-title":"Sci Rep"},{"key":"1823_CR10","doi-asserted-by":"publisher","first-page":"411","DOI":"10.13005\/bpj\/2141","volume":"14","author":"A Nagaraj","year":"2021","unstructured":"Nagaraj A, Wilson SA, Vaidyanathan L. Anti-Obesity properties of Calocybe indica in Zebra fishes with Short-Term High-Fat diet induction. Biomed Pharmacol J. 2021;14:411\u201323. https:\/\/doi.org\/10.13005\/bpj\/2141.","journal-title":"Biomed Pharmacol J"},{"key":"1823_CR11","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1038\/nmeth.3869","volume":"13","author":"BJ Callahan","year":"2016","unstructured":"Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJA, Holmes SP. DADA2: High-Resolution sample inference from illumina amplicon data. Nat Methods. 2016;13:581\u20133. https:\/\/doi.org\/10.1038\/nmeth.3869.","journal-title":"Nat Methods"},{"key":"1823_CR12","doi-asserted-by":"publisher","first-page":"D590","DOI":"10.1093\/nar\/gks1219","volume":"41","author":"C Quast","year":"2012","unstructured":"Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Gl\u00f6ckner FO. The SILVA ribosomal RNA gene database project: improved data processing and Web-Based tools. Nucleic Acids Res. 2012;41:D590\u20136. https:\/\/doi.org\/10.1093\/nar\/gks1219.","journal-title":"Nucleic Acids Res"},{"key":"1823_CR13","doi-asserted-by":"publisher","first-page":"317","DOI":"10.3389\/fmars.2019.00317","volume":"6","author":"JE Duffy","year":"2019","unstructured":"Duffy JE, Benedetti-Cecchi L, Trinanes J, Muller-Karger FE, Ambo-Rappe R, Bostr\u00f6m C, Buschmann AH, Byrnes J, Coles RG, Creed J, et al. Toward a coordinated global observing system for seagrasses and marine macroalgae. Front Mar Sci. 2019;6:317. https:\/\/doi.org\/10.3389\/fmars.2019.00317.","journal-title":"Front Mar Sci"},{"key":"1823_CR14","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1016\/j.sajb.2020.10.018","volume":"137","author":"DH Kim","year":"2021","unstructured":"Kim DH, Mahomoodally MF, Sadeer NB, Seok PG, Zengin G, Palaniveloo K, Khalil AA, Rauf A, Rengasamy KR. Nutritional and bioactive potential of seagrasses: A review. South Afr J Bot. 2021;137:216\u201327. https:\/\/doi.org\/10.1016\/j.sajb.2020.10.018.","journal-title":"South Afr J Bot"},{"key":"1823_CR15","doi-asserted-by":"publisher","first-page":"1082","DOI":"10.3390\/molecules29051082","volume":"29","author":"YW Prajoko","year":"2024","unstructured":"Prajoko YW, Qhabibi FR, Gerardo TS, Kizzandy K, Tanjaya K, Willyanto SE, Permatasari HK, Surya R, Mayulu N, Taslim NA, et al. Revealing novel source of breast Cancer inhibitors from seagrass Enhalus acoroides: in Silico and in vitro studies. Mol Basel Switz. 2024;29:1082. https:\/\/doi.org\/10.3390\/molecules29051082.","journal-title":"Mol Basel Switz"},{"key":"1823_CR16","doi-asserted-by":"publisher","first-page":"832","DOI":"10.3390\/plants10050832","volume":"10","author":"\u017d Fredotovi\u0107","year":"2021","unstructured":"Fredotovi\u0107 \u017d, Puizina J, Nazli\u0107 M, Maravi\u0107 A, Ljubenkov I, Soldo B, Vuko E, Baji\u0107 D. Phytochemical characterization and screening of antioxidant, antimicrobial and antiproliferative properties of Allium \u00d7 cornutum Clementi and two varieties of Allium Cepa L. Peel extracts. Plants. 2021;10:832. https:\/\/doi.org\/10.3390\/plants10050832.","journal-title":"Plants"},{"key":"1823_CR17","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1186\/s12906-018-2232-7","volume":"18","author":"L De Vincenti","year":"2018","unstructured":"De Vincenti L, Glasenapp Y, Catt\u00f2 C, Villa F, Cappitelli F, Papenbrock J. Hindering the formation and promoting the dispersion of medical biofilms: Non-Lethal effects of seagrass extracts. BMC Complement Altern Med. 2018;18:168. https:\/\/doi.org\/10.1186\/s12906-018-2232-7.","journal-title":"BMC Complement Altern Med"},{"key":"1823_CR18","doi-asserted-by":"publisher","first-page":"204201882092600","DOI":"10.1177\/2042018820926000","volume":"11","author":"R Pu","year":"2020","unstructured":"Pu R, Shi D, Gan T, Ren X, Ba Y, Huo Y, Bai Y, Zheng T, Cheng N. Effects of Metformin in obesity treatment in different populations: A Meta-Analysis. Ther Adv Endocrinol Metab. 2020;11:2042018820926000. https:\/\/doi.org\/10.1177\/2042018820926000.","journal-title":"Ther Adv Endocrinol Metab"},{"key":"1823_CR19","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1007\/s13679-019-00335-3","volume":"8","author":"A Yerevanian","year":"2019","unstructured":"Yerevanian A, Soukas AA, Metformin. Mechanisms in human obesity and weight loss. Curr Obes Rep. 2019;8:156\u201364. https:\/\/doi.org\/10.1007\/s13679-019-00335-3.","journal-title":"Curr Obes Rep"},{"key":"1823_CR20","doi-asserted-by":"publisher","first-page":"492","DOI":"10.1038\/s41366-025-01729-1","volume":"49","author":"V Subramaniyan","year":"2025","unstructured":"Subramaniyan V, Hanim YU. Role of pancreatic lipase Inhibition in obesity treatment: mechanisms and challenges towards current insights and future directions. Int J Obes. 2025;49:492\u2013506. https:\/\/doi.org\/10.1038\/s41366-025-01729-1.","journal-title":"Int J Obes"},{"key":"1823_CR21","doi-asserted-by":"publisher","unstructured":"Rahminiwati M, Iswantini D, Trivadila; Sianipar RNR, Sukma RM, Indariani S, Murni A. The strong Inhibition of pancreatic lipase by selected Indonesian medicinal plants as Anti-Obesity agents. Curr Issues Mol Biol. 2025;47. https:\/\/doi.org\/10.3390\/cimb47010039.","DOI":"10.3390\/cimb47010039"},{"key":"1823_CR22","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.54544","author":"N Sarvesh","year":"2024","unstructured":"Sarvesh N, Afeeza K, Suresh V, Dilipan E. Development of the antioxidant property of seagrass Extract-Based hydrogel for dental application. Cureus. 2024. https:\/\/doi.org\/10.7759\/cureus.54544.","journal-title":"Cureus"},{"key":"1823_CR23","doi-asserted-by":"publisher","DOI":"10.12692\/ijb\/16.6.178-184","author":"T Efren","year":"2020","unstructured":"Efren T, Oliva P, Canencia; Romeo M. Del rosario; Elvinia R. Alivio antioxidant activity of methanol extracts of Enhalus Acoroides and Thalassia Hemprichii from the coastal water of carmen, Agusan Del norte, Philippines. Int J Biosci IJB. 2020. https:\/\/doi.org\/10.12692\/ijb\/16.6.178-184.","journal-title":"Int J Biosci IJB"},{"key":"1823_CR24","doi-asserted-by":"publisher","first-page":"128119","DOI":"10.1016\/j.foodchem.2020.128119","volume":"338","author":"K Papoutsis","year":"2021","unstructured":"Papoutsis K, Zhang J, Bowyer MC, Brunton N, Gibney ER, Lyng J, Fruit. Vegetables, and mushrooms for the Preparation of extracts with \u03b1-Amylase and \u03b1-Glucosidase Inhibition properties: A review. Food Chem. 2021;338:128119. https:\/\/doi.org\/10.1016\/j.foodchem.2020.128119.","journal-title":"Food Chem"},{"key":"1823_CR25","doi-asserted-by":"publisher","first-page":"413","DOI":"10.5219\/911","volume":"12","author":"OB Afolabi","year":"2018","unstructured":"Afolabi OB, Oloyede OI, Ojo AA, Onansanya AA, Agunbiade SO, Ajiboye BO, Johnson J, Peters OA. In vitro antioxidant potential and inhibitory effect of Hydro-Ethanolic extract from African black Velvet tamarind (Dialium Indium) pulp on type 2 diabetes linked enzymes. Potravinarstvo Slovak J Food Sci. 2018;12:413\u201321. https:\/\/doi.org\/10.5219\/911.","journal-title":"Potravinarstvo Slovak J Food Sci"},{"key":"1823_CR26","doi-asserted-by":"publisher","unstructured":"Puspitasari YE, Tuenter E, Breynaert A, Foubert K, Herawati H, Hariati AM, Aulanni\u2019am A, De Bruyne T, Hermans N. \u03b1-Glucosidase inhibitory activity of tea and Kombucha from Rhizophora Mucronata leaves. Beverages. 2024;10. https:\/\/doi.org\/10.3390\/beverages10010022.","DOI":"10.3390\/beverages10010022"},{"key":"1823_CR27","doi-asserted-by":"publisher","first-page":"1290","DOI":"10.3889\/oamjms.2022.10167","volume":"10","author":"F Fauziah","year":"2022","unstructured":"Fauziah F, Ali H, Ilmiawati C, Bakhtra D, Agustin Z, Handayani D. Inhibitory activity of \u03b1-Glucosidase by the extract and fraction of marine Sponge-Derived fungus penicillium citrinum Xt6. Open Access Maced J Med Sci. 2022;10:1290\u20133. https:\/\/doi.org\/10.3889\/oamjms.2022.10167.","journal-title":"Open Access Maced J Med Sci"},{"key":"1823_CR28","doi-asserted-by":"publisher","first-page":"126","DOI":"10.20902\/IJCTR.2018.111016","volume":"11","author":"M Shanmugavadivu","year":"2018","unstructured":"Shanmugavadivu M, Velmurugan BK. Antibacterial activity and antidiabetic activity of Costus igneus, Gymnema Sylvestre and Ocimum Sanctum. Int J ChemTech Res. 2018;11:126\u201334. https:\/\/doi.org\/10.20902\/IJCTR.2018.111016.","journal-title":"Int J ChemTech Res"},{"key":"1823_CR29","doi-asserted-by":"publisher","unstructured":"Moiz A, Filion KB, Tsoukas MA, Yu OH, Peters TM, Eisenberg MJ. Mechanisms of GLP-1 receptor Agonist-Induced weight loss: A review of central and peripheral pathways in appetite and energy regulation. Am J Med. S 2025;0002934325000592. https:\/\/doi.org\/10.1016\/j.amjmed.2025.01.021.","DOI":"10.1016\/j.amjmed.2025.01.021"},{"key":"1823_CR30","doi-asserted-by":"publisher","first-page":"1715","DOI":"10.3390\/microorganisms8111715","volume":"8","author":"S Stojanov","year":"2020","unstructured":"Stojanov S, Berlec A, \u0160trukelj B. The influence of probiotics on the firmicutes\/bacteroidetes ratio in the treatment of obesity and inflammatory bowel disease. Microorganisms. 2020;8:1715. https:\/\/doi.org\/10.3390\/microorganisms8111715.","journal-title":"Microorganisms"},{"key":"1823_CR31","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1038\/s41579-020-0433-9","volume":"19","author":"Y Fan","year":"2021","unstructured":"Fan Y, Pedersen O. Gut microbiota in human metabolic health and disease. Nat Rev Microbiol. 2021;19:55\u201371. https:\/\/doi.org\/10.1038\/s41579-020-0433-9.","journal-title":"Nat Rev Microbiol"},{"key":"1823_CR32","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1016\/j.gtc.2016.07.001","volume":"45","author":"KB Martinez","year":"2016","unstructured":"Martinez KB, Pierre JF, Chang EB. The gut microbiota: the gateway to improved metabolism. Gastroenterol Clin North Am. 2016;45:601\u201314. https:\/\/doi.org\/10.1016\/j.gtc.2016.07.001.","journal-title":"Gastroenterol Clin North Am"}],"container-title":["Diabetology & Metabolic Syndrome"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13098-025-01823-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13098-025-01823-4\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13098-025-01823-4.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,21]],"date-time":"2025-06-21T15:02:51Z","timestamp":1750518171000},"score":1,"resource":{"primary":{"URL":"https:\/\/dmsjournal.biomedcentral.com\/articles\/10.1186\/s13098-025-01823-4"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,21]]},"references-count":32,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["1823"],"URL":"https:\/\/doi.org\/10.1186\/s13098-025-01823-4","relation":{},"ISSN":["1758-5996"],"issn-type":[{"value":"1758-5996","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,21]]},"assertion":[{"value":"28 May 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 June 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 June 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to publish"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Informed consent"}},{"value":"During the revision of this work the author(s) used ChatGPT in order to correct the language and paraphrase the necessary sections. After using this tool\/service, the author(s) reviewed and edited the content as necessary and took full responsibility for the content of the published article. Authors are ultimately responsible and accountable for the contents of the work.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"AI statements"}},{"value":"All procedures adhered strictly to the Guidelines for Reporting In Vivo Experiments (ARRIVE) and have also been reviewed and accepted in the Preclinical Trials Study Register with number PCTE0000604.","order":6,"name":"Ethics","group":{"name":"EthicsHeading","label":"Institutional review board statement"}},{"value":"The authors declare no competing interests.","order":7,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"235"}}