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The ability to produce endospores and biofilms contribute to their pathogenicity, classified in two types of food poisoning: diarrheal and emetic syndromes. Here we report gap-free, whole-genome sequences of two <jats:italic>B. cereus<\/jats:italic> strains isolated from air samples and analyse their emetic and diarrheal potential.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Genome assemblies of the <jats:italic>B. cereus<\/jats:italic> strains consist of one chromosome and seven plasmids each. The genome size of strain SGAir0260 is 6.30-Mb with 6590 predicted coding sequences (CDS) and strain SGAir0263 is 6.47-Mb with 6811 predicted CDS. Macrosynteny analysis showed 99% collinearity between the strains isolated from air and 90.2% with the reference genome. Comparative genomics with 57 complete <jats:italic>B. cereus<\/jats:italic> genomes suggests these strains from air are closely associated with strains isolated from foodborne illnesses outbreaks. Due to virulence potential of <jats:italic>B. cereus<\/jats:italic> and its reported involvement in nosocomial infections, antibiotic resistance analyses were performed and confirmed resistance to ampicillin and fosfomycin, with susceptibility to ciprofloxacin, tetracycline and vancomycin in both strains.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Phylogenetic analysis combined with detection of haemolytic (<jats:italic>hblA, hblC,<\/jats:italic> and <jats:italic>hblD<\/jats:italic>) and non-haemolytic (<jats:italic>nheA, nheB<\/jats:italic>, and <jats:italic>nheC<\/jats:italic>) enterotoxin genes in both air-isolated strains point to the diarrheic potential of the air isolates, though not emetic. Characterization of these airborne strains and investigation of their potential disease-causing genes could facilitate identification of environmental sources of contamination leading to foodborne illnesses and nosocomial infections transported by air.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13099-021-00399-4","type":"journal-article","created":{"date-parts":[[2021,1,30]],"date-time":"2021-01-30T09:03:17Z","timestamp":1611997397000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The genomic characterisation and comparison of Bacillus cereus strains isolated from indoor air"],"prefix":"10.1186","volume":"13","author":[{"given":"Balakrishnan N. 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