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Although the acute infection of\n                      <jats:italic>C. jejuni<\/jats:italic>\n                      (campylobacteriosis) is commonly treated with macrolides and fluoroquinolones, the emergence of antibiotic resistance among\n                      <jats:italic>C. jejuni<\/jats:italic>\n                      warrants the need for an alternative approach to control campylobacteriosis in humans. To this end, vaccines remain a safe, effective, and widely accepted strategy for controlling emerging and re-emerging infectious diseases. In search of a suitable vaccine against campylobacteriosis, recently, we demonstrated the potential of recombinant Haemolysin co-regulated protein (Hcp) of\n                      <jats:italic>C. jejuni<\/jats:italic>\n                      Type VI secretion system (T6SS) in imparting significant immune-protection against cecal colonization of\n                      <jats:italic>C. jejuni;<\/jats:italic>\n                      however, in the avian model. Since clinical features of human campylobacteriosis are more complicated than the avians, we explored the potential of Hcp as a T6SS targeted vaccine in a murine model as a more reliable and reproducible experimental host to study vaccine-induced immune-protection against\n                      <jats:italic>C. jejuni<\/jats:italic>\n                      . Because\n                      <jats:italic>C. jejuni<\/jats:italic>\n                      primarily utilizes the mucosal route for host pathogenesis, we analyzed the immunogenicity of a mucosally deliverable bioengineered Lactic acid bacteria (LAB),\n                      <jats:italic>Lactococcus lactis<\/jats:italic>\n                      (\n                      <jats:italic>L. lactis<\/jats:italic>\n                      ), expressing Hcp. Considering the role of Hcp in both structural (membrane-bound) and functional (effector protein) exhibition of\n                      <jats:italic>C. jejuni<\/jats:italic>\n                      T6SS, a head-to-head comparison of two different forms of recombinant LAB vectors (cell wall anchored and secreted form of Hcp) were tested and assessed for the immune phenotypes of each modality in BALB\/c mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We show that regardless of the Hcp protein localization, mucosal delivery of bioengineered LAB vector expressing Hcp induced high-level production of antigen-specific neutralizing antibody (sIgA) in the gut with the potential to reduce the cecal load of\n                      <jats:italic>C. jejuni<\/jats:italic>\n                      in mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Together with the non-commensal nature of\n                      <jats:italic>L. lactis,<\/jats:italic>\n                      short gut transit time in humans, and the ability to express the heterologous protein in the gut, the present study highlights the benefits of bioengineered LAB vectors based mucosal vaccine modality against\n                      <jats:italic>C. jejuni<\/jats:italic>\n                      without the risk of immunotolerance.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13099-021-00444-2","type":"journal-article","created":{"date-parts":[[2021,7,30]],"date-time":"2021-07-30T07:02:57Z","timestamp":1627628577000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Bioengineering of LAB vector expressing Haemolysin co-regulated protein (Hcp): a strategic approach to control gut colonization of Campylobacter jejuni in a murine model"],"prefix":"10.1186","volume":"13","author":[{"given":"Chandan","family":"Gorain","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afruja","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankita","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samiran","family":"Mondal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2265-9856","authenticated-orcid":false,"given":"Amirul Islam","family":"Mallick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,7,30]]},"reference":[{"key":"444_CR1","first-page":"705","volume":"70","author":"C Bern","year":"1992","unstructured":"Bern C, Martines J, de Zoysa I, Glass RI. 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