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Additionally, to assess fecal composition of <jats:italic>Lactobacillus acidophilus ATCC-4356<\/jats:italic> and <jats:italic>Bifidobacterium bifidum ATCC-11863<\/jats:italic> microbiota genotypes<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Material and methods<\/jats:title>\n                <jats:p>This study was carried out on 40 non-cirrhotic chronically infected HCV patients, and 10 healthy-controls. Liver biopsy and HCV genomic viral load were assessed for all patients after full clinical examination. <jats:italic>Lactobacillus acidophilus ATCC-4356<\/jats:italic> and <jats:italic>Bifidobacterium bifidum ATCC-11863<\/jats:italic> microbiota were assessed in all fecal samples using PCR assay, after counting total lactic acid bacteria.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>There was a significantly higher difference between the count of both total lactic acid and <jats:italic>Lactobacillus acidophilus<\/jats:italic> of healthy controls compared to patients (P-value\u2009&lt;\u20090.001). Though the count of total lactic acid bacteria, and <jats:italic>Lactobacillus acidophilus<\/jats:italic> were higher in the cases with early stage of fibrosis (score\u2009\u2264\u20091) compared to those with score\u2009&gt;\u20091, there were no statistically significant differences with both the serum level of hepatitis C viremia (P\u2009=\u20090.850 and 0.977 respectively) and the score of fibrosis (P\u2009=\u20090.246 and 0.260 respectively). Genotypic analysis for the composition of the studied microbiota revealed that diversity was higher in healthy controls compared to patients.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The progression of hepatic fibrosis in HCV chronically infected patients seems to be plausible based on finding the altered <jats:italic>Lactobacillus acidophilus<\/jats:italic> and <jats:italic>Bifidobacterium bifidum<\/jats:italic> gut microbiota composition. Thus, modulation of these microbiota seems to be a promising target for prevention and control of HCV infection.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13099-022-00501-4","type":"journal-article","created":{"date-parts":[[2022,6,15]],"date-time":"2022-06-15T05:02:39Z","timestamp":1655269359000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Potential impact of gut Lactobacillus acidophilus and Bifidobacterium bifidum on hepatic histopathological changes in non-cirrhotic hepatitis C virus patients with different viral load"],"prefix":"10.1186","volume":"14","author":[{"given":"Zeinab","family":"Ashour","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasha","family":"Shahin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeinab","family":"Ali-Eldin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed","family":"El-Shayeb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarek","family":"El-Tayeb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salwa","family":"Bakr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,6,15]]},"reference":[{"issue":"10","key":"501_CR1","doi-asserted-by":"publisher","first-page":"1457","DOI":"10.3390\/nu10101457","volume":"10","author":"X Meng","year":"2018","unstructured":"Meng X, Li S, Li Y, Gan RY, Li HB. 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