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This study investigated transcriptomic adaptation of tissues two weeks after experimental coinfections with <jats:italic>Ascaridia galli, Heterakis gallinarum<\/jats:italic>, and <jats:italic>H. meleagridis<\/jats:italic> in Lohmann Brown (LB), Lohmann Dual (LD), and Ross-308 (R) male chickens, which differ in growth rates (R\u2009&gt;\u2009LD\u2009&gt;\u2009LB).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>RNA sequencing of the jejunum, caecum, and liver tissues revealed tissue-specific, strain-dependent transcriptional responses. Coinfection was confirmed during necropsy, and Ascaris-specific antibodies as well as alpha-1-acid glycoprotein were significantly higher in infected birds (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). The caecum exhibited the highest unique differentially expressed genes, DEGs (n\u2009=\u20094,094), corresponding to significant activation of complex immune pathways and inhibition of metabolic pathways (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). Jejunum DEGs (n\u2009=\u2009760) primarily related to muscle contraction, collagen metabolism notably in LB and LD strains. The liver displayed fewer unique DEGs (n\u2009=\u2009266) but prominently activated immune responses, especially in R chickens.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>In general, slower-performing strains effectively initiated responses favouring worm expulsion and tissue repair in the jejunum, whereas high-performing strains predominantly showed inflammatory responses in the caecum and liver. These findings highlight tissue-specific adaptations underlying strain-dependent tolerance to coinfections with mixed parasites.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/s13099-025-00716-1","type":"journal-article","created":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T19:04:11Z","timestamp":1749668651000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Tissue-specific transcriptomic adaptation in three strains of chickens during coinfections with parasites"],"prefix":"10.1186","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4997-4589","authenticated-orcid":false,"given":"Oyekunle John","family":"Oladosu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6470-0434","authenticated-orcid":false,"given":"Henry","family":"Reyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3214-2498","authenticated-orcid":false,"given":"Nares","family":"Trakooljul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6044-5368","authenticated-orcid":false,"given":"Solvig","family":"G\u00f6rs","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9498-6020","authenticated-orcid":false,"given":"Cornelia C.","family":"Metges","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6690-0050","authenticated-orcid":false,"given":"G\u00fcrb\u00fcz","family":"Da\u015f","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,6,11]]},"reference":[{"key":"716_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/J.PSJ.2025.104770","volume":"104","author":"MZ Akram","year":"2025","unstructured":"Akram MZ, Sureda EA, Corion M, et al. 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