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However, highly sensitive detection methods are still needed to distinguish different forms of leishmaniasis. In the present study, we developed a quantitative real-time polymerase chain reaction (PCR) to detect and quantify\n                      <jats:italic>Leishmania tropica<\/jats:italic>\n                      parasites in paraffin-embedded tissue samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The ability of real-time PCR for leishmania detection was higher than histopathological evaluation. The quantitative real-time PCR (qPCR) quantified parasite loads were highly correlated with microscopic results (\n                      <jats:italic>r\u2009<\/jats:italic>\n                      =\u20090.598;\n                      <jats:italic>P\u2009<\/jats:italic>\n                      &lt;\u20090.001). Among patients, the parasite load was inversely correlated with disease duration (acute CL lesions had very higher parasite load than chronic CL lesions), but there was no difference in the parasite load according to the patients\u2019 age and sex as well as location of the lesions. In contrast to Ridley scoring system (P\u2009&lt;\u20090.001), there were no statistically significant differences in the relative number of parasites among the lupoid and non-lupoid forms of chronic lesions in real-time PCR (P\u2009=\u20090.549), which indicates the superiority of histopathological evaluation for chronic forms differentiation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13104-019-4666-5","type":"journal-article","created":{"date-parts":[[2019,9,23]],"date-time":"2019-09-23T09:02:42Z","timestamp":1569229362000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Ability of real-time PCR for differential diagnosis of various forms of cutaneous leishmaniasis: a comparative study with histopathology"],"prefix":"10.1186","volume":"12","author":[{"given":"Maryam","family":"Fekri-SoofiAbadi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meisam","family":"Fekri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alireza","family":"moradabadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Vahidi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simin","family":"Shamsi-Meymandi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donya","family":"Dabiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahriar","family":"Dabiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,9,23]]},"reference":[{"issue":"9496","key":"4666_CR1","doi-asserted-by":"publisher","first-page":"1561","DOI":"10.1016\/S0140-6736(05)67629-5","volume":"366","author":"HW Murray","year":"2005","unstructured":"Murray HW, Berman JD, Davies CR, Saravia NG. 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Informed consent was obtained from all the participants prior to enrolment.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"615"}}