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Rickettsial antigens were presented equally well both with formaldehyde and all paraformaldehyde-based fixations, but only protocol with 4% paraformaldehyde in PHEM buffer allowed accurate imaging of actin filaments, and simultaneously allows monitoring of rickettsiae using actin-based motility during infection inside the host cells.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s13104-019-4699-9","type":"journal-article","created":{"date-parts":[[2019,10,16]],"date-time":"2019-10-16T09:03:15Z","timestamp":1571216595000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Optimized fixation of actin filaments for improved indirect immunofluorescence staining of rickettsiae"],"prefix":"10.1186","volume":"12","author":[{"given":"Monika","family":"Danchenko","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia","family":"Csaderova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre Edouard","family":"Fournier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2597-4405","authenticated-orcid":false,"given":"Zuzana","family":"Sekeyova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,10,16]]},"reference":[{"key":"4699_CR1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc03a05s11","author":"NC Ammerman","year":"2008","unstructured":"Ammerman NC, Beier-Sexton M, Azad AF. 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The experiments were performed under standard conditions in the experimental animal facility of the Biomedical Research Center, Slovak Academy of Sciences.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"657"}}