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We therefore wanted to establish a quantitative confocal imaging assay to measure endo-reticular membrane expansion following <jats:italic>Salmonella<\/jats:italic> infections of host cells.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Data description<\/jats:title>\n<jats:p>High-content screening confocal fluorescence microscopic image set of <jats:italic>Salmonella<\/jats:italic> infected HeLa cells is presented. The images were collected with a PerkinElmer Opera LX high-content screening system in seven 96-well plates, 50 field-of-views and DAPI, endoplasmic reticulum tracker channels and <jats:italic>Salmonella<\/jats:italic> mCherry protein in each well. Totally 93,300 confocal fluorescence microscopic images were published in this dataset. An <jats:italic>ImageJ<\/jats:italic> high-content image analysis workflow was used to extract features. Cells were classified as infected and non-infected, the mean intensity of endoplasmic reticulum tracker under <jats:italic>Salmonella<\/jats:italic> bacteria was calculated. Statistical analysis was performed by an <jats:italic>R<\/jats:italic> script, quantifying infected and non-infected cells for wild-type and \u0394<jats:italic>sifA<\/jats:italic> mutant cells. The dataset can be further used by researchers working with big data of endoplasmic reticulum fluorescence microscopic images, <jats:italic>Salmonella<\/jats:italic> bacterial infection images and human cancer cells.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s13104-019-4844-5","type":"journal-article","created":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T18:03:00Z","timestamp":1576519380000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["High-content screening image dataset and quantitative image analysis of Salmonella infected human cells"],"prefix":"10.1186","volume":"12","author":[{"given":"Antony N.","family":"Antoniou","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon J.","family":"Powis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0149-145X","authenticated-orcid":false,"given":"Janos","family":"Kriston-Vizi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,12,16]]},"reference":[{"key":"4844_CR1","doi-asserted-by":"publisher","first-page":"1096","DOI":"10.1016\/0140-6736(92)90675-S","volume":"339","author":"O Maki-Ikola","year":"1992","unstructured":"Maki-Ikola O, Granfors K. 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