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Three of the six sera poorly supported growth of the murine pathogen<jats:italic>Chlamydia muridarum<\/jats:italic>, resulting in 73\u201390% reduction in progeny formation. Most significantly, the one with the strongest (90%)<jats:italic>C. muridarum<\/jats:italic>inhibition activity showed optimal<jats:italic>C. trachomatis<\/jats:italic>-supporting efficiency. These findings indicate that in laboratories that study multiple<jats:italic>Chlamydia<\/jats:italic>species, serum samples should be prescreened on a species basis. Considering<jats:italic>Chlamydial<\/jats:italic>biology and epidemiology, it may even be necessary to perform serum tests on a serovar- or strain-basis for studying some animal chlamydiae.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s13104-020-4893-9","type":"journal-article","created":{"date-parts":[[2020,1,13]],"date-time":"2020-01-13T18:03:17Z","timestamp":1578938597000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Optimal cultivation of Chlamydia requires testing of serum on individual species"],"prefix":"10.1186","volume":"13","author":[{"given":"Malhar","family":"Desai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huirong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4903-926X","authenticated-orcid":false,"given":"Huizhou","family":"Fan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,1,13]]},"reference":[{"issue":"2","key":"4893_CR1","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1002\/rmb2.12024","volume":"16","author":"T Yao","year":"2017","unstructured":"Yao T, Asayama Y. 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