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Methicillin-resistant\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      (MRSA) is the main PJI-causing bacteria. Previously, we had reported the combined effect of Ag-HA coating and vancomycin (VCM) on MRSA biofilm formation 24\u00a0h after MRSA inoculation. In this study, we investigated the time-dependent efficacy of Ag-HA coating and VCM on MRSA biofilm formation on Ti discs in vitro by three-dimensional confocal laser scanning microscopic analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      For the Ti VCM and HA VCM groups, the total biofilm volumes per area at 96\u00a0h after MRSA inoculation were significantly larger than those at 48\u00a0h after MRSA inoculation, respectively (p\u2009&lt;\u20090.001). In contrast, for the Ag-HA VCM group, the total biofilm volume per area at 96\u00a0h was significantly smaller than that at 48\u00a0h (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). Moreover, 96\u00a0h after MRSA inoculation, the total biofilm volume per area of the Ag-HA VCM groups was significantly smaller than those of the Ti VCM and HA VCM groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). Thus, the combination of Ag-HA and VCM might be useful for the prevention of MRSA-associated PJI.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s13104-021-05499-7","type":"journal-article","created":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T08:04:15Z","timestamp":1614672255000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Time-dependent efficacy of combination of silver-containing hydroxyapatite coating and vancomycin on methicillin-resistant Staphylococcus aureus\u00a0biofilm formation in vitro"],"prefix":"10.1186","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2473-2463","authenticated-orcid":false,"given":"Akira","family":"Hashimoto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Miyamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakumo","family":"Kii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoki","family":"Kobatake","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeo","family":"Shobuike","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iwao","family":"Noda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motoki","family":"Sonohata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaaki","family":"Mawatari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,3,2]]},"reference":[{"issue":"26","key":"5499_CR1","first-page":"E5094","volume":"114","author":"Y Wang","year":"2017","unstructured":"Wang Y, Cheng LI, Helfer DR, Ashbaugh AG, Miller RJ, Tzomides AJ, et al. 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