{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T22:23:53Z","timestamp":1773267833170,"version":"3.50.1"},"reference-count":45,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,5,26]],"date-time":"2021-05-26T00:00:00Z","timestamp":1621987200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,5,26]],"date-time":"2021-05-26T00:00:00Z","timestamp":1621987200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Res Notes"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Objective<\/jats:title>\n                <jats:p>Retinoschisis and Norrie disease are X-linked recessive retinal disorders caused by mutations in <jats:italic>RS1<\/jats:italic> and <jats:italic>NDP<\/jats:italic> genes respectively. Both are likely to be monogenic and no locus heterogeneity has been reported. However, there are reports showing overlapping features of Norrie disease and retinoschisis in a <jats:italic>NDP<\/jats:italic> knock-out mouse model and also the involvement of both the genes in retinoschisis patients. Yet, the exact molecular relationships between the two disorders have still not been\u00a0understood. The study investigated the association between retinoschisin (RS1) and norrin (NDP) using\u00a0in vitro\u00a0and\u00a0in silico\u00a0approaches. Specific protein\u2013protein interaction between RS1 and NDP was analyzed in human retina by co-immunoprecipitation assay and MALDI-TOF mass spectrometry. STRING database was used to explore the functional relationship.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Result<\/jats:title>\n                <jats:p>Co-immunoprecipitation demonstrated lack of a direct interaction between RS1 and NDP\u00a0and\u00a0was further substantiated by mass spectrometry. However, STRING revealed a potential indirect functional association between the two proteins.\u00a0Progressively, our analyses indicate that FZD4 protein interactome via PLIN2 as well as the MAP kinase signaling pathway to be a likely link bridging the functional relationship between retinoschisis and Norrie disease.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s13104-021-05617-5","type":"journal-article","created":{"date-parts":[[2021,5,26]],"date-time":"2021-05-26T20:03:20Z","timestamp":1622059400000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Retinoschisis and Norrie disease: a missing link"],"prefix":"10.1186","volume":"14","author":[{"given":"Rahini","family":"Rajendran","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dhandayuthapani","family":"Sudha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subbulakshmi","family":"Chidambaram","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hemavathy","family":"Nagarajan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Umashankar","family":"Vetrivel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6318-225X","authenticated-orcid":false,"given":"Jayamuruga Pandian","family":"Arunachalam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,5,26]]},"reference":[{"issue":"7","key":"5617_CR1","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1136\/bjo.79.7.697","volume":"79","author":"N George","year":"1995","unstructured":"George N, Yates J, Moore A. X linked retinoschisis. Br J Ophthalmol. 1995;79(7):697\u2013702. https:\/\/doi.org\/10.1136\/bjo.79.7.697.","journal-title":"Br J Ophthalmol"},{"issue":"2","key":"5617_CR2","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1038\/ng1097-164","volume":"17","author":"C Sauer","year":"1997","unstructured":"Sauer C, Gehrig A, Warneke-Wittstock R, Marquardt A, Ewing C, Gibson A, et al. Positional cloning of the gene associated with X-linked juvenile retinoschisis. Nat Genet. 1997;17(2):164\u201370. https:\/\/doi.org\/10.1038\/ng1097-164.","journal-title":"Nat Genet"},{"issue":"3","key":"5617_CR3","first-page":"816","volume":"42","author":"L Molday","year":"2001","unstructured":"Molday L, Hicks D, Sauer C, Weber B, Molday R. Expression of X-linked retinoschisis protein RS1 in photoreceptor and bipolar cells. Invest Ophthalmol Vis Sci. 2001;42(3):816\u201325.","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"2","key":"5617_CR4","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1016\/j.preteyeres.2011.12.002","volume":"84","author":"R Molday","year":"2007","unstructured":"Molday R. Focus on molecules: retinoschisin (RS1). Exp Eye Res. 2007;84(2):227\u20138. https:\/\/doi.org\/10.1016\/j.preteyeres.2011.12.002.","journal-title":"Exp Eye Res"},{"key":"5617_CR5","first-page":"1108","volume":"12","author":"Y Takada","year":"2006","unstructured":"Takada Y, Fariss RN, Muller M, Bush RA, Rushing EJ, Sieving PA. Retinoschisin expression and localization in rodent and human pineal and consequences of mouse RS1 gene knockout. Mol Vis. 2006;12:1108\u201316.","journal-title":"Mol Vis"},{"issue":"6","key":"5617_CR6","doi-asserted-by":"publisher","first-page":"794","DOI":"10.1001\/jamaophthalmol.2013.2047","volume":"131","author":"R Silva","year":"2013","unstructured":"Silva R, Berrocal A, Lam B, Albini T. Novel mutation in BEST1 associated with retinoschisis. JAMA Ophthalmology. 2013;131(6):794\u20138. https:\/\/doi.org\/10.1001\/jamaophthalmol.2013.2047.","journal-title":"JAMA Ophthalmology"},{"key":"5617_CR7","first-page":"487","volume":"21","author":"N Wang","year":"2015","unstructured":"Wang N, Liu L, Chen H, Tsai S, Chang T, Tsai T, Yang C, Chao A, Chen K, Kao L, Yeung L. Clinical presentations of X-linked retinoschisis in Taiwanese patients confirmed with genetic sequencing. Mol Vis. 2015;21:487\u2013501.","journal-title":"Mol Vis"},{"key":"5617_CR8","first-page":"1054","volume":"12","author":"J Hung","year":"1980","unstructured":"Hung J, Hilton G. Neovascular glaucoma in a patient with X-linked juvenile retinoschisis. Ann Ophthalmol. 1980;12:1054\u20135.","journal-title":"Ann Ophthalmol"},{"issue":"2","key":"5617_CR9","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1159\/000050844","volume":"215","author":"D Berinstein","year":"2001","unstructured":"Berinstein D, Hiraoka M, Trese M, Shastry B. Coats\u2019 disease and congenital retinoschisis in a single eye: a case report and DNA analysis. Ophthalmologica. 2001;215(2):132\u20135. https:\/\/doi.org\/10.1159\/000050844.","journal-title":"Ophthalmologica"},{"issue":"3","key":"5617_CR10","first-page":"117","volume":"7","author":"M Warburg","year":"1971","unstructured":"Warburg M. Norrie disease. Birth defects. 1971;7(3):117\u201324.","journal-title":"Birth defects"},{"issue":"3","key":"5617_CR11","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1038\/ng0692-199","volume":"1","author":"W Berger","year":"1992","unstructured":"Berger W, Meindl A, van de Pol T, Cremers F, Ropers H, D\u00f6erner C, et al. Isolation of a candidate gene for Norrie disease by positional cloning. Nat Genet. 1992;1(3):199\u2013203. https:\/\/doi.org\/10.1038\/ng0692-199.","journal-title":"Nat Genet"},{"issue":"8","key":"5617_CR12","doi-asserted-by":"publisher","first-page":"1909","DOI":"10.1002\/ajmg.a.35469","volume":"158A","author":"S Smith","year":"2012","unstructured":"Smith S, Mullen T, Graham D, Sims K, Rehm H. Norrie disease: extraocular clinical manifestations in 56 patients. Am J Med Genet A. 2012;158A(8):1909\u201317. https:\/\/doi.org\/10.1002\/ajmg.a.35469.","journal-title":"Am J Med Genet A"},{"issue":"2","key":"5617_CR13","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1038\/ng1092-139","volume":"2","author":"A Meindl","year":"1992","unstructured":"Meindl A, Berger W, Meitinger T, van de Pol D, Achatz H, D\u00f6rner C, et al. Norrie disease is caused by mutations in an extracellular protein resembling C\u2013terminal globular domain of mucins. Nat Genet. 1992;2(2):139\u201343. https:\/\/doi.org\/10.1038\/ng1092-139.","journal-title":"Nat Genet"},{"issue":"2","key":"5617_CR14","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1016\/j.cell.2009.07.047","volume":"139","author":"X Ye","year":"2009","unstructured":"Ye X, Wang Y, Cahill H, Yu M, Badea T, Smallwood P, et al. Norrin, Frizzled-4, and Lrp5 signaling in endothelial cells controls a genetic program for retinal vascularization. Cell. 2009;139(2):285\u201398. https:\/\/doi.org\/10.1016\/j.cell.2009.07.047.","journal-title":"Cell"},{"issue":"11","key":"5617_CR15","doi-asserted-by":"publisher","first-page":"2031","DOI":"10.1093\/hmg\/8.11.2031","volume":"8","author":"G Black","year":"1999","unstructured":"Black G, Perveen R, Bonshek R, Cahill M, Clayton-Smith J, Lloyd IC, McLeod D. Coats\u2019 disease of the retina (unilateral retinal telangiectasis) caused by somatic mutation in the NDP gene: a role for norrin in retinal angiogenesis. Hum Mol Genet. 1999;8(11):2031\u20135. https:\/\/doi.org\/10.1093\/hmg\/8.11.2031.","journal-title":"Hum Mol Genet"},{"issue":"2","key":"5617_CR16","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1038\/ng1093-180","volume":"5","author":"Z Chen","year":"1993","unstructured":"Chen Z, Battinelli E, Fielder A, Bundey S, Sims K, Breakefield X, et al. A mutation in the Norrie disease gene (NDP) associated with X\u2013linked familial exudative vitreoretinopathy. Nat Genet. 1993;5(2):180\u20133. https:\/\/doi.org\/10.1038\/ng1093-180.","journal-title":"Nat Genet"},{"issue":"5","key":"5617_CR17","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1001\/archopht.1997.01100150653015","volume":"115","author":"B Shastry","year":"1997","unstructured":"Shastry B, Pendergast S, Hartzer M, Liu X, Trese M. Identification of missense mutations in the norrie disease gene associated with advanced retinopathy of prematurity. Arch Ophthalmol. 1997;115(5):651\u20135. https:\/\/doi.org\/10.1001\/archopht.1997.01100150653015.","journal-title":"Arch Ophthalmol"},{"issue":"2","key":"5617_CR18","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1080\/13816810802705755","volume":"30","author":"E Aponte","year":"2009","unstructured":"Aponte E, Pulido J, Ellison J, Quiram P, Mohney B. A novel NDP mutation in an infant with unilateral persistent fetal vasculature and retinal vasculopathy. Ophthalmic Genet. 2009;30(2):99\u2013102. https:\/\/doi.org\/10.1080\/13816810802705755.","journal-title":"Ophthalmic Genet"},{"issue":"3","key":"5617_CR19","first-page":"710","volume":"38","author":"K Ruether","year":"1997","unstructured":"Ruether K, van de Pol D, Jaissle G, Berger W, Tornow R, Zrenner E. Retinoschisis like alterations in the mouse eye caused by gene targeting of the Norrie disease gene. Invest Ophthalmol Vis Sci. 1997;38(3):710\u20138.","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"2","key":"5617_CR20","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1007\/s100380170108","volume":"46","author":"M Hiraoka","year":"2001","unstructured":"Hiraoka M, Rossi F, Trese M, Shastry B. X-linked juvenile retinoschisis: mutations at the retinoschisis and Norrie disease gene loci? J Hum Genet. 2001;46(2):53\u20136. https:\/\/doi.org\/10.1007\/s100380170108.","journal-title":"J Hum Genet"},{"issue":"4","key":"5617_CR21","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1111\/j.1399-0004.1981.tb01031.x","volume":"20","author":"V Godel","year":"1981","unstructured":"Godel V, Goodman R. X-Linked recessive primary retinal dysplasia: clinical findings in affected males and carrier females. Clin Genet. 1981;20(4):260\u20136. https:\/\/doi.org\/10.1111\/j.1399-0004.1981.tb01031.x.","journal-title":"Clin Genet"},{"issue":"1","key":"5617_CR22","doi-asserted-by":"publisher","first-page":"R9.1","DOI":"10.1186\/gb-2008-9-1-r9","volume":"9","author":"B Bockler","year":"2008","unstructured":"Bockler B, Bateman A. Protein interactions in human genetic diseases. Genome Biol. 2008;9(1):R9.1-R9.12. https:\/\/doi.org\/10.1186\/gb-2008-9-1-r9.","journal-title":"Genome Biol"},{"issue":"4","key":"5617_CR23","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1101\/gr.071852.107","volume":"18","author":"T Ideker","year":"2008","unstructured":"Ideker T, Sharan R. Protein networks in disease. Genome Res. 2008;18(4):644\u201352. https:\/\/doi.org\/10.1101\/gr.071852.107.","journal-title":"Genome Res"},{"key":"5617_CR24","doi-asserted-by":"publisher","first-page":"32792","DOI":"10.1074\/jbc.M706321200","volume":"282","author":"L Molday","year":"2007","unstructured":"Molday L, Wu W, Molday R. Retinoschisin (RS1), the protein encoded by the X-linked retinoschisis gene, is anchored to the surface of retinal photoreceptor and bipolar cells through its interactions with a Na\/K ATPase-SARM1 complex. J Biol Chem. 2007;282:32792\u2013801. https:\/\/doi.org\/10.1074\/jbc.M706321200.","journal-title":"J Biol Chem"},{"key":"5617_CR25","doi-asserted-by":"publisher","first-page":"3966","DOI":"10.1074\/jbc.M806333200","volume":"284","author":"L Shi","year":"2009","unstructured":"Shi L, Jian K, Ko M, Trump D, Ko G. Retinoschisin, a new binding partner for L-type voltage-gated calcium channels in the retina. J Biol Chem. 2009;284:3966\u201375. https:\/\/doi.org\/10.1074\/jbc.M806333200.","journal-title":"J Biol Chem"},{"key":"5617_CR26","first-page":"892","volume":"12","author":"M Steiner-Champliaud","year":"2006","unstructured":"Steiner-Champliaud M, Sahel J, Hicks D. Retinoschisin forms a multi-molecular complex with extracellular matrix and cytoplasmic proteins: interactions with beta2 laminin and alphaB-crystallin. Mol Vis. 2006;12:892\u2013901.","journal-title":"Mol Vis"},{"issue":"9","key":"5617_CR27","doi-asserted-by":"publisher","first-page":"2060","DOI":"10.1242\/jcs.123471","volume":"126","author":"C Deng","year":"2013","unstructured":"Deng C, Reddy P, Cheng Y, Luo C, Hsiao C, Hsueh A. Multi-functional norrin is a ligand for the LGR4 receptor. J Cell Sci. 2013;126(9):2060\u20138. https:\/\/doi.org\/10.1242\/jcs.123471.","journal-title":"J Cell Sci"},{"issue":"6","key":"5617_CR28","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1016\/s0092-8674(04)00216-8","volume":"116","author":"Q Xu","year":"2004","unstructured":"Xu Q, Wang Y, Dabdoub A, Smallwood P, Williams J, Woods C, Kelley M, Jiang L, Tasman W, Zhang K, Nathans J. Vascular development in the retina and inner ear: control by Norrin and Frizzled-4, a high-affinity ligand-receptor pair. Cell. 2004;116(6):883\u201395. https:\/\/doi.org\/10.1016\/s0092-8674(04)00216-8.","journal-title":"Cell"},{"issue":"21","key":"5617_CR29","doi-asserted-by":"publisher","first-page":"2305","DOI":"10.1101\/gad.228544.113","volume":"27","author":"K Jiyuan","year":"2013","unstructured":"Jiyuan K, Kaleeckal G, Harikumar C, Chen C, Xin G, Liren W, Parker N, Cheng Z, Xu W, Williams B, Melcher K, Miller L, Xu E. Structure and function of Norrin in assembly and activation of a Frizzled 4\u2013Lrp5\/6 complex. Genes Dev. 2013;27(21):2305\u201319. https:\/\/doi.org\/10.1101\/gad.228544.113.","journal-title":"Genes Dev"},{"issue":"2","key":"5617_CR30","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1016\/j.cell.2009.07.048","volume":"139","author":"H Junge","year":"2009","unstructured":"Junge H, Yang S, Burton J, Paes K, Shu X, French D, Costa M, Rice D, Ye W. TSPAN12 regulates retinal vascular development by promoting Norrin\u2014but not Wnt-induced FZD4\/beta-catenin signaling. Cell. 2009;139(2):299\u2013311. https:\/\/doi.org\/10.1016\/j.cell.2009.07.048.","journal-title":"Cell"},{"key":"5617_CR31","doi-asserted-by":"publisher","first-page":"3551","DOI":"10.1002\/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2","volume":"20","author":"D Perkins","year":"1999","unstructured":"Perkins D, Pappin D, Creasy D, Cottrell J. Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis. 1999;20:3551\u201367. https:\/\/doi.org\/10.1002\/(SICI)1522-2683(19991201)20:18%3c3551::AID-ELPS3551%3e3.0.CO;2-2.","journal-title":"Electrophoresis"},{"issue":"15","key":"5617_CR32","doi-asserted-by":"publisher","first-page":"2597","DOI":"10.1002\/pmic.201400515","volume":"15","author":"M Pathan","year":"2015","unstructured":"Pathan M, Keerthikumar S, Ang C, Gangoda L, Quek C, Williamson N, Mouradov D, Sieber O, Simpson R, Salim A, Bacic A. FunRich: a standalone tool for functional enrichment analysis. Proteomics. 2015;15(15):2597\u2013601. https:\/\/doi.org\/10.1002\/pmic.201400515.","journal-title":"Proteomics"},{"issue":"18","key":"5617_CR33","doi-asserted-by":"publisher","first-page":"3442","DOI":"10.1093\/nar\/28.18.3442","volume":"28","author":"B Snel","year":"2000","unstructured":"Snel B, Lehmann G, Bork P, Huynen M. STRING: a web-server to retrieve and display the repeatedly occurring neighbourhood of a gene. Nucleic Acids Res. 2000;28(18):3442\u20134. https:\/\/doi.org\/10.1093\/nar\/28.18.3442.","journal-title":"Nucleic Acids Res"},{"issue":"7325","key":"5617_CR34","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1038\/468851a","volume":"468","author":"L Bonetta","year":"2010","unstructured":"Bonetta L. Protein-protein interactions: interactome under construction. Nature. 2010;468(7325):851\u20134. https:\/\/doi.org\/10.1038\/468851a.","journal-title":"Nature"},{"key":"5617_CR35","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/147648","author":"V Rao","year":"2014","unstructured":"Rao V, Srinivas K, Sujini G, Kumar G. Protein\u2013protein interaction detection: methods and analysis. Int J Proteomics. 2014. https:\/\/doi.org\/10.1155\/2014\/147648.","journal-title":"Int J Proteomics"},{"issue":"18","key":"5617_CR36","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.csbj.2014.08.008","volume":"11","author":"T Sevimoglu","year":"2014","unstructured":"Sevimoglu T, Kazim Y. The role of protein interaction networks in systems biomedicine. Comput Struct Biotechnol J. 2014;11(18):22\u20137. https:\/\/doi.org\/10.1016\/j.csbj.2014.08.008.","journal-title":"Comput Struct Biotechnol J"},{"issue":"21","key":"5617_CR37","doi-asserted-by":"publisher","first-page":"2305","DOI":"10.1101\/gad.228544.113","volume":"27","author":"J Ke","year":"2013","unstructured":"Ke J, Harikumar KG, Erice C, Chen C, Gu X, Wang L, Parker N, Cheng Z, Xu W, Williams BO, Melcher K, Miller LJ, Xu HE. Structure and function of Norrin in assembly and activation of a Frizzled 4-Lrp5\/6 complex. Genes Dev. 2013;27(21):2305\u201319. https:\/\/doi.org\/10.1101\/gad.228544.113.","journal-title":"Genes Dev"},{"issue":"1","key":"5617_CR38","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.atherosclerosis.2014.08.038","volume":"237","author":"TP Leren","year":"2014","unstructured":"Leren TP. Sorting an LDL receptor with bound PCSK9 to intracellular degradation. Atherosclerosis. 2014;237(1):76\u201381. https:\/\/doi.org\/10.1016\/j.atherosclerosis.2014.08.038.","journal-title":"Atherosclerosis"},{"issue":"7","key":"5617_CR39","doi-asserted-by":"publisher","first-page":"C728","DOI":"10.1152\/ajpcell.00448.2011","volume":"303","author":"A McIntosh","year":"2012","unstructured":"McIntosh A, Senthivinayagam S, Moon K, Gupta S, Lwande J, Murphy C, et al. Direct interaction of Plin2 with lipids on the surface of lipid droplets: a live cell FRET analysis. Am J Physiol Cell Physiol. 2012;303(7):C728\u201342. https:\/\/doi.org\/10.1152\/ajpcell.00448.2011.","journal-title":"Am J Physiol Cell Physiol"},{"key":"5617_CR40","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1167\/iovs.06-0641","volume":"48","author":"A Gehrig","year":"2007","unstructured":"Gehrig A, Langmann T, Horling F, Janssen A, Bonin M, Walter M, Poths S, Weber B. Genome-wide expression profiling of the retinoschisin-deficient retina in early postnatal mouse development. Invest Ophthalmol Vis Sci. 2007;48:891\u2013900. https:\/\/doi.org\/10.1167\/iovs.06-0641.","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"2","key":"5617_CR41","doi-asserted-by":"publisher","first-page":"906","DOI":"10.1167\/iovs.08-1731","volume":"50","author":"N Schafer","year":"2009","unstructured":"Schafer N, Luhmann U, Feil S, Berger W. Differential gene expression in Ndph-knockout mice in retinal development. Invest Ophthalmol Vis Sci. 2009;50(2):906\u201316. https:\/\/doi.org\/10.1167\/iovs.08-1731.","journal-title":"Invest Ophthalmol Vis Sci"},{"issue":"4","key":"5617_CR42","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1111\/jcmm.13019","volume":"21","author":"K Plossl","year":"2017","unstructured":"Plossl K, Weber B, Friedrich U. The X-linked juvenile retinoschisis protein retinoschisin is a novel regulator of mitogen-activated protein kinase signalling and apoptosis in the retina. J Cell Mol Med. 2017;21(4):768\u201380. https:\/\/doi.org\/10.1111\/jcmm.13019.","journal-title":"J Cell Mol Med"},{"issue":"1","key":"5617_CR43","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/S1556-0864(15)31506-9","volume":"1","author":"J Molina","year":"2006","unstructured":"Molina J, Adjei A. The Ras\/Raf\/MAPK pathway. J Thorac Oncol. 2006;1(1):7\u20139.","journal-title":"J Thorac Oncol"},{"issue":"3","key":"5617_CR44","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1177\/1947601911407329","volume":"2","author":"N Neel","year":"2011","unstructured":"Neel N, Martin T, Stratford J, Zand T, Reiner D, Der C. The RalGEF-Ral effector signaling network the road less traveled for anti-Ras drug discovery. Genes Cancer. 2011;2(3):275\u201387. https:\/\/doi.org\/10.1177\/1947601911407329.","journal-title":"Genes Cancer"},{"key":"5617_CR45","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/j.jbior.2014.09.007","volume":"57","author":"S Bulley","year":"2015","unstructured":"Bulley S, Clarke J, Droubi A, Giudici M, Irvine R. Exploring phosphatidylinositol 5-phosphate 4-kinase function. Adv Biol Regul. 2015;57:193\u2013202. https:\/\/doi.org\/10.1016\/j.jbior.2014.09.007.","journal-title":"Adv Biol Regul"}],"container-title":["BMC Research Notes"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13104-021-05617-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s13104-021-05617-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s13104-021-05617-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,26]],"date-time":"2021-05-26T20:18:22Z","timestamp":1622060302000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcresnotes.biomedcentral.com\/articles\/10.1186\/s13104-021-05617-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,26]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["5617"],"URL":"https:\/\/doi.org\/10.1186\/s13104-021-05617-5","relation":{},"ISSN":["1756-0500"],"issn-type":[{"value":"1756-0500","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,26]]},"assertion":[{"value":"22 December 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 May 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 May 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All experiments involving the usage of human donor eye tissues were approved by Institutional Review Board and Ethics Committee of Vision Research Foundation (Ref no. 202-2009-P) and adhered to the tenets of declaration of Helsinki.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"All the authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interest"}}],"article-number":"204"}}